LOCUS       pQE-5.        3414 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bacterial expression vector with a T5 promoter. For other reading 
            frames, use pQE-3 or pQE-4.
ACCESSION   .
VERSION     .
KEYWORDS    pQE-5
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Qiagen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Discontinued pQE vector.
FEATURES             Location/Qualifiers
     source          1..3414
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             101..106
                     /note="ribosome binding site"
     CDS             115..117
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     misc_feature    119..144
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     misc_feature    144..154
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     terminator      160..254
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             298..954
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      1022..1108
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    1264..1404
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1590..2178)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2352..3209)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3210..3314)
                     /label=AmpR promoter
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgagg
      121 atccgtcgac ctgcagccaa gcttaattag ctgagcttgg actcctgttg atagatccag
      181 taatgacctc agaactccat ctggatttgt tcagaacgct cggttgccgc cgggcgtttt
      241 ttattggtga gaatccaagc tagcttggcg agattttcag gagctaagga agctaaaatg
      301 gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat
      361 tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt
      421 acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac
      481 attcttgccc gcctgatgaa tgctcatccg gaatttcgta tggcaatgaa agacggtgag
      541 ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg
      601 ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca catatattcg
      661 caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat
      721 atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc
      781 aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac
      841 aaggtgctga tgccgctggc gattcaggtt catcatgccg tctgtgatgg cttccatgtc
      901 ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt
      961 ttttaaggca gttattggtg cccttaaacg cctggggtaa tgactctcta gcttgaggca
     1021 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     1081 ggtgaacgct ctcctgagta ggacaaatcc gccgctctag agctgcctcg cgcgtttcgg
     1141 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     1201 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     1261 gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg
     1321 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
     1381 gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc
     1441 tcggtctgtc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     1501 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     1561 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     1621 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     1681 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     1741 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg
     1801 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     1861 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     1921 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     1981 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     2041 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     2101 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     2161 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     2221 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     2281 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     2341 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     2401 tcatccatag ctgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     2461 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     2521 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     2581 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     2641 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     2701 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     2761 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     2821 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     2881 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     2941 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     3001 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     3061 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     3121 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     3181 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     3241 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     3301 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     3361 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct tcac
//
