LOCUS       pQE-30_Xa.        3509 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bacterial vector for expressing N-terminally 6xHis-tagged proteins 
            with a Factor Xa cleavage site.
ACCESSION   .
VERSION     .
KEYWORDS    pQE-30 Xa
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Qiagen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Clone at StuI to insert a coding sequence just after the Factor Xa 
            cleavage site.
FEATURES             Location/Qualifiers
     source          1..3509
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             101..106
                     /note="ribosome binding site"
     CDS             115..117
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             127..144
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             166..177
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     misc_feature    240..250
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     terminator      256..350
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             394..1050
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      1118..1204
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    1359..1499
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1685..2273)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2447..3304)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3305..3409)
                     /label=AmpR promoter
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga
      121 ggatcgcatc accatcacca tcacggatct ggctctggat ctggtatcga gggaaggcct
      181 tataatggaa ctggatccgc atgcgagctc ggtaccccgg gtcgacctgc agccaagctt
      241 aattagctga gcttggactc ctgttgatag atccagtaat gacctcagaa ctccatctgg
      301 atttgttcag aacgctcggt tgccgccggg cgttttttat tggtgagaat ccaagctagc
      361 ttggcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc
      421 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct
      481 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag
      541 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct
      601 catccggaat ttcgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac
      661 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac
      721 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa
      781 aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc
      841 tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc
      901 gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt
      961 caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa
     1021 cagtactgcg atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct
     1081 taaacgcctg gggtaatgac tctctagctt gaggcatcaa ataaaacgaa aggctcagtc
     1141 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac
     1201 aaatccgccc tctagagctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     1261 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     1321 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
     1381 agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag
     1441 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     1501 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     1561 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     1621 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     1681 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     1741 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     1801 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     1861 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     1921 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     1981 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     2041 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     2101 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     2161 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     2221 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     2281 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     2341 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     2401 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     2461 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     2521 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     2581 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     2641 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     2701 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     2761 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     2821 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     2881 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     2941 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     3001 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     3061 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     3121 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     3181 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     3241 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     3301 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     3361 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     3421 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     3481 ggcgtatcac gaggcccttt cgtcttcac
//
