LOCUS       pQE-30_UA.        3504 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bacterial expression and 6xHis-tagging vector for UA cloning of a 
            PCR product.
ACCESSION   .
VERSION     .
KEYWORDS    pQE-30 UA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Qiagen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     This vector is supplied in a linearized form, with bases 174-175 
            replaced with 3'-U overhangs.
FEATURES             Location/Qualifiers
     source          1..3504
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             101..106
                     /note="ribosome binding site"
     CDS             115..117
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             127..144
                     /label=6xHis
                     /note="6xHis affinity tag"
     misc_feature    145..235
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     misc_feature    235..245
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     terminator      251..345
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             389..1045
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      1113..1199
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    1354..1494
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1680..2268)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2442..3299)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3300..3404)
                     /label=AmpR promoter
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga
      121 ggatcgcatc accatcacca tcacggatcc cacgtgatat cctcaatcgc ttctagaagc
      181 gattgaggag atctgagctc ggtacccggg tcgacaggcc tctgcagcca agcttaatta
      241 gctgagcttg gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg
      301 ttcagaacgc tcggttgccg ccgggcgttt tttattggtg agaatccaag ctagcttggc
      361 gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt
      421 tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg
      481 tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa
      541 taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc
      601 ggaatttcgt atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg
      661 ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga
      721 cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct
      781 ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt
      841 gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt
      901 caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt
      961 tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta
     1021 ctgcgatgag tggcagggcg gggcgtaatt tttttaaggc agttattggt gcccttaaac
     1081 gcctggggta atgactctct agcttgaggc atcaaataaa acgaaaggct cagtcgaaag
     1141 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc
     1201 cgccctctag agctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     1261 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     1321 gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga
     1381 tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac
     1441 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct
     1501 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     1561 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     1621 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     1681 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     1741 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     1801 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     1861 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     1921 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     1981 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     2041 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     2101 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     2161 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     2221 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     2281 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     2341 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     2401 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     2461 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     2521 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     2581 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     2641 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     2701 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     2761 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     2821 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     2881 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     2941 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     3001 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     3061 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     3121 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     3181 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     3241 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     3301 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     3361 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     3421 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     3481 atcacgaggc cctttcgtct tcac
//
