LOCUS       pQE-22.        3998 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bacterial vector for expressing DHFR fusion proteins with a 
            C-terminal 6xHis tag. Similar to pQE-16 and pQE-19, but with a 
            different reading frame after the DHFR.
ACCESSION   .
VERSION     .
KEYWORDS    pQE-22
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Qiagen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3998)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Discontinued pQE vector.
FEATURES             Location/Qualifiers
     source          1..3998
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             101..106
                     /note="ribosome binding site"
     CDS             115..117
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             133..690
                     /label=DHFR
                     /note="mouse dihydrofolate reductase"
     CDS             704..721
                     /label=6xHis
                     /note="6xHis affinity tag"
     misc_feature    728..738
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     terminator      744..838
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             882..1538
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      1606..1692
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    1848..1988
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2174..2762)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2936..3793)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3794..3898)
                     /label=AmpR promoter
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga
      121 ggatccggca tcatggttcg accattgaac tcgatcgtcg ccgtgtccca aaatatgggg
      181 attggcaaga acggagacct accctggcct ccgctcagga acgagttcaa gtacttccaa
      241 agaatgacca caacctcttc agtggaaggt aaacagaatc tggtgattat gggtaggaaa
      301 acctggttct ccattcctga gaagaatcga cctttaaagg acagaattaa tatagttctc
      361 agtagagaac tcaaagaacc accacgagga gctcattttc ttgccaaaag tttggatgat
      421 gccttaagac ttattgaaca accggaattg gcaagtaaag tagacatggt ttggatagtc
      481 ggaggcagtt ctgtttacca ggaagccatg aatcaaccag gccaccttag actctttgtg
      541 acaaggatca tgcaggaatt tgaaagtgac acgtttttcc cagaaattga tttggggaaa
      601 tataaacttc tcccagaata cccaggcgtc ctctctgagg tccaggagga aaaaggcatc
      661 aagtataagt ttgaagtcta cgagaagaaa ggttggaaga tctcatcacc atcaccatca
      721 ctaagcttaa ttagctgagc ttggactcct gttgatagat ccagtaatga cctcagaact
      781 ccatctggat ttgttcagaa cgctcggttg ccgccgggcg ttttttattg gtgagaatcc
      841 aagctagctt ggcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg
      901 gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt
      961 cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga
     1021 ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga
     1081 tgaatgctca tccggaattt cgtatggcaa tgaaagacgg tgagctggtg atatgggata
     1141 gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga
     1201 gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt
     1261 acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag
     1321 ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct
     1381 tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc
     1441 tggcgattca ggttcatcat gccgtctgtg atggcttcca tgtcggcaga atgcttaatg
     1501 aattacaaca gtactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt
     1561 ggtgccctta aacgcctggg gtaatgactc tctagcttga ggcatcaaat aaaacgaaag
     1621 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
     1681 agtaggacaa atccgccgct ctagagctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     1741 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     1801 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     1861 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
     1921 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     1981 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc tgtcggctgc
     2041 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     2101 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     2161 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     2221 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     2281 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     2341 tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt
     2401 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     2461 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     2521 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     2581 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     2641 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     2701 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     2761 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     2821 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     2881 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     2941 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagctgcct
     3001 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     3061 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     3121 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     3181 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     3241 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     3301 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     3361 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     3421 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     3481 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     3541 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     3601 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     3661 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     3721 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     3781 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     3841 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     3901 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     3961 ataaaaatag gcgtatcacg aggccctttc gtcttcac
//
