LOCUS       pQE-19.        3996 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bacterial vector for expressing DHFR fusion proteins with a 
            C-terminal 6xHis tag. Similar to pQE-16 and pQE-22, but with a 
            different reading frame after the DHFR.
ACCESSION   .
VERSION     .
KEYWORDS    pQE-19
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Qiagen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Discontinued pQE vector.
FEATURES             Location/Qualifiers
     source          1..3996
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             101..106
                     /note="ribosome binding site"
     CDS             115..117
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             133..690
                     /label=DHFR
                     /note="mouse dihydrofolate reductase"
     CDS             702..719
                     /label=6xHis
                     /note="6xHis affinity tag"
     misc_feature    726..736
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     terminator      742..836
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             880..1536
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      1604..1690
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    1846..1986
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2172..2760)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2934..3791)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3792..3896)
                     /label=AmpR promoter
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga
      121 ggatccggca tcatggttcg accattgaac tcgatcgtcg ccgtgtccca aaatatgggg
      181 attggcaaga acggagacct accctggcct ccgctcagga acgagttcaa gtacttccaa
      241 agaatgacca caacctcttc agtggaaggt aaacagaatc tggtgattat gggtaggaaa
      301 acctggttct ccattcctga gaagaatcga cctttaaagg acagaattaa tatagttctc
      361 agtagagaac tcaaagaacc accacgagga gctcattttc ttgccaaaag tttggatgat
      421 gccttaagac ttattgaaca accggaattg gcaagtaaag tagacatggt ttggatagtc
      481 ggaggcagtt ctgtttacca ggaagccatg aatcaaccag gccaccttag actctttgtg
      541 acaaggatca tgcaggaatt tgaaagtgac acgtttttcc cagaaattga tttggggaaa
      601 tataaacttc tcccagaata cccaggcgtc ctctctgagg tccaggagga aaaaggcatc
      661 aagtataagt ttgaagtcta cgagaagaaa ggttcagatc tcatcaccat caccatcact
      721 aagcttaatt agctgagctt ggactcctgt tgatagatcc agtaatgacc tcagaactcc
      781 atctggattt gttcagaacg ctcggttgcc gccgggcgtt ttttattggt gagaatccaa
      841 gctagcttgg cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga
      901 tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca
      961 gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc
     1021 gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg
     1081 aatgctcatc cggaatttcg tatggcaatg aaagacggtg agctggtgat atgggatagt
     1141 gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt
     1201 gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac
     1261 ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc
     1321 aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc
     1381 gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg
     1441 gcgattcagg ttcatcatgc cgtctgtgat ggcttccatg tcggcagaat gcttaatgaa
     1501 ttacaacagt actgcgatga gtggcagggc ggggcgtaat ttttttaagg cagttattgg
     1561 tgcccttaaa cgcctggggt aatgactctc tagcttgagg catcaaataa aacgaaaggc
     1621 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag
     1681 taggacaaat ccgccgctct agagctgcct cgcgcgtttc ggtgatgacg gtgaaaacct
     1741 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     1801 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca
     1861 gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta
     1921 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     1981 atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtctg tcggctgcgg
     2041 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     2101 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     2161 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     2221 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     2281 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     2341 ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag
     2401 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     2461 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     2521 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     2581 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     2641 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     2701 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     2761 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     2821 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     2881 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     2941 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agctgcctga
     3001 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     3061 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     3121 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     3181 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     3241 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     3301 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     3361 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     3421 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     3481 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     3541 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     3601 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     3661 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     3721 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     3781 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     3841 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     3901 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     3961 aaaaataggc gtatcacgag gccctttcgt cttcac
//
