LOCUS       pQE-18.        4005 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bacterial vector for expressing DHFR fusion proteins. Similar to 
            pQE-17, but with a different reading frame after the DHFR.
ACCESSION   .
VERSION     .
KEYWORDS    pQE-18
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Qiagen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Discontinued pQE vector.
FEATURES             Location/Qualifiers
     source          1..4005
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             101..106
                     /note="ribosome binding site"
     CDS             115..117
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             133..690
                     /label=DHFR
                     /note="mouse dihydrofolate reductase"
     CDS             711..728
                     /label=6xHis
                     /note="6xHis affinity tag"
     misc_feature    735..745
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     terminator      751..845
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             889..1545
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      1613..1699
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    1855..1995
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2181..2769)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2943..3800)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3801..3905)
                     /label=AmpR promoter
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga
      121 ggatccggca tcatggttcg accattgaac tcgatcgtcg ccgtgtccca aaatatgggg
      181 attggcaaga acggagacct accctggcct ccgctcagga acgagttcaa gtacttccaa
      241 agaatgacca caacctcttc agtggaaggt aaacagaatc tggtgattat gggtaggaaa
      301 acctggttct ccattcctga gaagaatcga cctttaaagg acagaattaa tatagttctc
      361 agtagagaac tcaaagaacc accacgagga gctcattttc ttgccaaaag tttggatgat
      421 gccttaagac ttattgaaca accggaattg gcaagtaaag tagacatggt ttggatagtc
      481 ggaggcagtt ctgtttacca ggaagccatg aatcaaccag gccaccttag actctttgtg
      541 acaaggatca tgcaggaatt tgaaagtgac acgtttttcc cagaaattga tttggggaaa
      601 tataaacttc tcccagaata cccaggcgtc ctctctgagg tccaggagga aaaaggcatc
      661 aagtataagt ttgaagtcta cgagaagaaa ggttccagat cttccgatct catcaccatc
      721 accatcacta agcttaatta gctgagcttg gactcctgtt gatagatcca gtaatgacct
      781 cagaactcca tctggatttg ttcagaacgc tcggttgccg ccgggcgttt tttattggtg
      841 agaatccaag ctagcttggc gagattttca ggagctaagg aagctaaaat ggagaaaaaa
      901 atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca
      961 tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt
     1021 ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc
     1081 cgcctgatga atgctcatcc ggaatttcgt atggcaatga aagacggtga gctggtgata
     1141 tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg
     1201 ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg
     1261 gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc
     1321 gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac
     1381 aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg
     1441 atgccgctgg cgattcaggt tcatcatgcc gtctgtgatg gcttccatgt cggcagaatg
     1501 cttaatgaat tacaacagta ctgcgatgag tggcagggcg gggcgtaatt tttttaaggc
     1561 agttattggt gcccttaaac gcctggggta atgactctct agcttgaggc atcaaataaa
     1621 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     1681 tctcctgagt aggacaaatc cgccgctcta gagctgcctc gcgcgtttcg gtgatgacgg
     1741 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     1801 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
     1861 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag
     1921 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     1981 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtctgt
     2041 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     2101 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     2161 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     2221 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     2281 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     2341 gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt
     2401 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     2461 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     2521 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     2581 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     2641 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     2701 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     2761 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     2821 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     2881 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     2941 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     3001 gctgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     3061 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     3121 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     3181 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     3241 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     3301 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     3361 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     3421 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     3481 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     3541 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     3601 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     3661 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     3721 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     3781 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     3841 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     3901 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     3961 ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcac
//
