LOCUS       pQE-15.        4004 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bacterial vector for expressing DHFR fusion proteins with an 
            N-terminal 6xHis tag. For other reading frames after DHFR, using 
            pQE-13 or pQE-14.
ACCESSION   .
VERSION     .
KEYWORDS    pQE-15
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Qiagen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Discontinued pQE vector.
FEATURES             Location/Qualifiers
     source          1..4004
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             101..106
                     /note="ribosome binding site"
     CDS             115..117
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             127..144
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             157..714
                     /label=DHFR
                     /note="mouse dihydrofolate reductase"
     misc_feature    728..744
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     terminator      750..844
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             888..1544
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      1612..1698
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    1854..1994
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2180..2768)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2942..3799)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3800..3904)
                     /label=AmpR promoter
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga
      121 ggatcgcatc accatcacca tcacggatcc ggcatcatgg ttcgaccatt gaactcgatc
      181 gtcgccgtgt cccaaaatat ggggattggc aagaacggag acctaccctg gcctccgctc
      241 aggaacgagt tcaagtactt ccaaagaatg accacaacct cttcagtgga aggtaaacag
      301 aatctggtga ttatgggtag gaaaacctgg ttctccattc ctgagaagaa tcgaccttta
      361 aaggacagaa ttaatatagt tctcagtaga gaactcaaag aaccaccacg aggagctcat
      421 tttcttgcca aaagtttgga tgatgcctta agacttattg aacaaccgga attggcaagt
      481 aaagtagaca tggtttggat agtcggaggc agttctgttt accaggaagc catgaatcaa
      541 ccaggccacc ttagactctt tgtgacaagg atcatgcagg aatttgaaag tgacacgttt
      601 ttcccagaaa ttgatttggg gaaatataaa cttctcccag aatacccagg cgtcctctct
      661 gaggtccagg aggaaaaagg catcaagtat aagtttgaag tctacgagaa gaaaggttgg
      721 aagatcttaa gcttaattag ctgagcttgg actcctgttg atagatccag taatgacctc
      781 agaactccat ctggatttgt tcagaacgct cggttgccgc cgggcgtttt ttattggtga
      841 gaatccaagc tagcttggcg agattttcag gagctaagga agctaaaatg gagaaaaaaa
      901 tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat
      961 ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt
     1021 taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc
     1081 gcctgatgaa tgctcatccg gaatttcgta tggcaatgaa agacggtgag ctggtgatat
     1141 gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc
     1201 tctggagtga ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg
     1261 cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg
     1321 tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca
     1381 acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga
     1441 tgccgctggc gattcaggtt catcatgccg tctgtgatgg cttccatgtc ggcagaatgc
     1501 ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt ttttaaggca
     1561 gttattggtg cccttaaacg cctggggtaa tgactctcta gcttgaggca tcaaataaaa
     1621 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     1681 ctcctgagta ggacaaatcc gccgctctag agctgcctcg cgcgtttcgg tgatgacggt
     1741 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     1801 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc
     1861 atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc
     1921 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     1981 aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtctgtc
     2041 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     2101 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     2161 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     2221 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     2281 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     2341 cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt
     2401 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     2461 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     2521 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     2581 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     2641 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     2701 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     2761 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     2821 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     2881 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     2941 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     3001 ctgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     3061 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     3121 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     3181 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     3241 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     3301 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     3361 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     3421 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     3481 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     3541 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     3601 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     3661 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     3721 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     3781 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     3841 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     3901 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     3961 taacctataa aaataggcgt atcacgaggc cctttcgtct tcac
//
