LOCUS       pQE-14.        4002 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bacterial vector for expressing DHFR fusion proteins with an 
            N-terminal 6xHis tag. For other reading frames after DHFR, using 
            pQE-13 or pQE-15.
ACCESSION   .
VERSION     .
KEYWORDS    pQE-14
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Qiagen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Discontinued pQE vector.
FEATURES             Location/Qualifiers
     source          1..4002
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             101..106
                     /note="ribosome binding site"
     CDS             115..117
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             127..144
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             157..714
                     /label=DHFR
                     /note="mouse dihydrofolate reductase"
     misc_feature    726..742
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     terminator      748..842
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             886..1542
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      1610..1696
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    1852..1992
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2178..2766)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2940..3797)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3798..3902)
                     /label=AmpR promoter
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga
      121 ggatcgcatc accatcacca tcacggatcc ggcatcatgg ttcgaccatt gaactcgatc
      181 gtcgccgtgt cccaaaatat ggggattggc aagaacggag acctaccctg gcctccgctc
      241 aggaacgagt tcaagtactt ccaaagaatg accacaacct cttcagtgga aggtaaacag
      301 aatctggtga ttatgggtag gaaaacctgg ttctccattc ctgagaagaa tcgaccttta
      361 aaggacagaa ttaatatagt tctcagtaga gaactcaaag aaccaccacg aggagctcat
      421 tttcttgcca aaagtttgga tgatgcctta agacttattg aacaaccgga attggcaagt
      481 aaagtagaca tggtttggat agtcggaggc agttctgttt accaggaagc catgaatcaa
      541 ccaggccacc ttagactctt tgtgacaagg atcatgcagg aatttgaaag tgacacgttt
      601 ttcccagaaa ttgatttggg gaaatataaa cttctcccag aatacccagg cgtcctctct
      661 gaggtccagg aggaaaaagg catcaagtat aagtttgaag tctacgagaa gaaaggttca
      721 gatcttaagc ttaattagct gagcttggac tcctgttgat agatccagta atgacctcag
      781 aactccatct ggatttgttc agaacgctcg gttgccgccg ggcgtttttt attggtgaga
      841 atccaagcta gcttggcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc
      901 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt
      961 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta
     1021 aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc
     1081 ctgatgaatg ctcatccgga atttcgtatg gcaatgaaag acggtgagct ggtgatatgg
     1141 gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc
     1201 tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg
     1261 tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc
     1321 tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac
     1381 ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg
     1441 ccgctggcga ttcaggttca tcatgccgtc tgtgatggct tccatgtcgg cagaatgctt
     1501 aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt
     1561 tattggtgcc cttaaacgcc tggggtaatg actctctagc ttgaggcatc aaataaaacg
     1621 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1681 cctgagtagg acaaatccgc cgctctagag ctgcctcgcg cgtttcggtg atgacggtga
     1741 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     1801 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
     1861 gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag
     1921 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     1981 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtctgtcgg
     2041 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     2101 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     2161 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     2221 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     2281 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     2341 tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg
     2401 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     2461 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     2521 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     2581 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     2641 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     2701 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     2761 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     2821 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     2881 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     2941 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagct
     3001 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     3061 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     3121 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     3181 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     3241 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     3301 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     3361 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     3421 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     3481 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     3541 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     3601 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     3661 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     3721 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     3781 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     3841 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     3901 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     3961 acctataaaa ataggcgtat cacgaggccc tttcgtcttc ac
//
