LOCUS       pQE-13.        4003 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bacterial vector for expressing DHFR fusion proteins with an 
            N-terminal 6xHis tag. For other reading frames after DHFR, using 
            pQE-14 or pQE-15.
ACCESSION   .
VERSION     .
KEYWORDS    pQE-13
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Qiagen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Discontinued pQE vector.
FEATURES             Location/Qualifiers
     source          1..4003
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             101..106
                     /note="ribosome binding site"
     CDS             115..117
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             127..144
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             157..714
                     /label=DHFR
                     /note="mouse dihydrofolate reductase"
     misc_feature    727..743
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     terminator      749..843
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             887..1543
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      1611..1697
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    1853..1993
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2179..2767)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2941..3798)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3799..3903)
                     /label=AmpR promoter
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga
      121 ggatcgcatc accatcacca tcacggatcc ggcatcatgg ttcgaccatt gaactcgatc
      181 gtcgccgtgt cccaaaatat ggggattggc aagaacggag acctaccctg gcctccgctc
      241 aggaacgagt tcaagtactt ccaaagaatg accacaacct cttcagtgga aggtaaacag
      301 aatctggtga ttatgggtag gaaaacctgg ttctccattc ctgagaagaa tcgaccttta
      361 aaggacagaa ttaatatagt tctcagtaga gaactcaaag aaccaccacg aggagctcat
      421 tttcttgcca aaagtttgga tgatgcctta agacttattg aacaaccgga attggcaagt
      481 aaagtagaca tggtttggat agtcggaggc agttctgttt accaggaagc catgaatcaa
      541 ccaggccacc ttagactctt tgtgacaagg atcatgcagg aatttgaaag tgacacgttt
      601 ttcccagaaa ttgatttggg gaaatataaa cttctcccag aatacccagg cgtcctctct
      661 gaggtccagg aggaaaaagg catcaagtat aagtttgaag tctacgagaa gaaaggttcc
      721 agatcttaag cttaattagc tgagcttgga ctcctgttga tagatccagt aatgacctca
      781 gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag
      841 aatccaagct agcttggcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat
      901 cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt
      961 tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt
     1021 aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg
     1081 cctgatgaat gctcatccgg aatttcgtat ggcaatgaaa gacggtgagc tggtgatatg
     1141 ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct
     1201 ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc
     1261 gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt
     1321 ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa
     1381 cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat
     1441 gccgctggcg attcaggttc atcatgccgt ctgtgatggc ttccatgtcg gcagaatgct
     1501 taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaatttt tttaaggcag
     1561 ttattggtgc ccttaaacgc ctggggtaat gactctctag cttgaggcat caaataaaac
     1621 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     1681 tcctgagtag gacaaatccg ccgctctaga gctgcctcgc gcgtttcggt gatgacggtg
     1741 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     1801 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
     1861 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
     1921 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     1981 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtctgtcg
     2041 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     2101 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     2161 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     2221 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     2281 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     2341 ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc
     2401 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     2461 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     2521 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     2581 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     2641 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     2701 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     2761 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     2821 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     2881 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     2941 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagc
     3001 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     3061 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     3121 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     3181 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     3241 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     3301 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     3361 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     3421 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     3481 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     3541 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     3601 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     3661 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     3721 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     3781 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     3841 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     3901 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     3961 aacctataaa aataggcgta tcacgaggcc ctttcgtctt cac
//
