LOCUS       pQC-tTS-IN.        8226 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Retroviral vector for expressing tTS, which represses expression 
            from Tet-responsive promoters in the absence of doxycycline.
ACCESSION   .
VERSION     .
KEYWORDS    pQC-tTS-IN
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8226)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8226)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..308
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             513..688
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    751..950
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             1151..1567
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
     enhancer        1604..1907
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1908..2111
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2262..3107
                     /label=tTS
                     /note="fusion of a hybrid tetracycline repressor TetR with
                     a nuclear localization signal and a KRAB silencing domain"
     misc_feature    3134..3720
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             3721..4512
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
     LTR             4932..5357
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     promoter        5623..5952
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      complement(6245..6833)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7007..7864)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7865..7969)
                     /label=AmpR promoter
     enhancer        8155..8226
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc tgggggatcg atcctctaga gtccgttaca taacttacgg
     1621 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     1681 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     1741 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     1801 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1861 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1921 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     1981 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     2041 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     2101 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     2161 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa
     2221 cggacctgca ggaattgatc cgcggccgca ccggtgccac catgtctaga ttagataaaa
     2281 gtaaagtgat taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa
     2341 cccgtaaact cgcccagaag ctaggtgtag agcagcctac attgtattgg cacgtgcgca
     2401 acaagcagac tcttatgaac atgctttcag aggcaatact ggcgaagcat cacacccgtt
     2461 cagcaccgtt accgactgag agttggcagc agtttctcca ggaaaatgct ctgagtttcc
     2521 gtaaagcatt actggtccat cgtgatggag cccgattgca tatagggacc tctcctacgc
     2581 ccccccagtt tgaacaagca gaggcgcaac tacgctgtct atgcgatgca gggttttcgg
     2641 tcgaggaggc tcttttcatt ctgcaatcta tcagccattt tacgttgggt gcagtattag
     2701 aggagcaagc aacaaaccag atagaaaata atcatgtgat agacgctgca ccaccattat
     2761 tacaagaggc atttaatatt caggcgagaa cctctgctga aatggccttc catttcgggc
     2821 tgaaatcatt aatatttgga ttttctgcac agttagatga aaaaaagcat acacccattg
     2881 aggatggtaa taaaccaaaa aagaagagaa agctagcagt gtcagtgaca tttgaagatg
     2941 tggctgtgct ctttactcgg gacgagtgga agaagctgga tctgtctcag agaagcctgt
     3001 accgtgaggt gatgctggag aattacagca acctggcctc catggcagga ttcctgttta
     3061 ccaaaccaaa ggtgatctcc ctgttgcagc aaggagagga tccctggtaa gaattccgcc
     3121 cccccccccc ccccccctct ccctcccccc cccctaacgt tactggccga agccgcttgg
     3181 aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca
     3241 atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc
     3301 ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag
     3361 cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg
     3421 gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac
     3481 aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa
     3541 gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc
     3601 tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc
     3661 ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataagct tgccacaacc
     3721 atggctgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     3781 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     3841 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     3901 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     3961 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     4021 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     4081 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     4141 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     4201 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     4261 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     4321 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     4381 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     4441 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     4501 gacgagttct tctgagcggg atcggctaga gtcgacccgg acgggacgct cctgcgcctg
     4561 atacagaacg aattgcttgc aggcatctca tgagtgtgtc ttcccgtttt ccgcctgagg
     4621 tcactgcgtg gatggagcgc tggcgcctgc tgcgcgacgg cgagctgctc accacccact
     4681 cgagggctgc agcgctgcag aggccgagtg cagaactgct ccaaagggac ctcaaggctt
     4741 tccgagggac actaggctga ctccatcgag ccagtgtaga gataagctta tcgattagtc
     4801 caatttgtta aagacaggat atcagtggtc caggctctag ttttgactca acaatatcac
     4861 cagctgaagc ctatagagta cgagccatag ataaaataaa agattttatt tagtctccag
     4921 aaaaaggggg gaatgaaaga ccccacctgt aggtttggca agctagctta agtaacgcca
     4981 ttttgcaagg catggaaaaa tacataactg agaatagaga agttcagatc aaggtcagga
     5041 acagatggaa cagggtcgac cctagagaac catcagatgt ttccagggtg ccccaaggac
     5101 ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg
     5161 cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg gggcgccagt
     5221 cctccgattg actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca
     5281 tccgacttgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc
     5341 agcgggggtc tttcatttgg gggctcgtcc gggatcggga gacccctgcc cagggaccac
     5401 cgacccacca ccgggaggta agctggctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     5461 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     5521 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     5581 cagtcacgta gcgatagcgg agtgtagatc cggctgtgga atgtgtgtca gttagggtgt
     5641 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     5701 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     5761 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     5821 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     5881 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     5941 ggcttttgca aaaagcttac tggcttaact atgcggcatc agagcagatt gtactgagag
     6001 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     6061 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     6121 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     6181 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     6241 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     6301 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     6361 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     6421 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     6481 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     6541 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     6601 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     6661 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     6721 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     6781 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     6841 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     6901 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     6961 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     7021 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     7081 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     7141 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     7201 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     7261 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg
     7321 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     7381 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     7441 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     7501 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     7561 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     7621 cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     7681 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     7741 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     7801 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     7861 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     7921 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     7981 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     8041 ggcgtatcac gaggcccttt cgtcttcaag aattagcttg gccattgcat acgttgtatc
     8101 catatcataa tatgtacatt tatattggct catgtccaac attaccgcca tgttgacatt
     8161 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     8221 tggagt
//
