LOCUS       40924_35753        5444 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Integration vector pPyr-Kan, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5444)
  AUTHORS   Middleton R, Hofmeister A.
  TITLE     New shuttle vectors for ectopic insertion of genes into Bacillus 
            subtilis
  JOURNAL   Plasmid 51 (3), 238-245 (2004)
  PUBMED    15109830
REFERENCE   2  (bases 1 to 5444)
  AUTHORS   Middleton RH, Hofmeister AEM.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2003) Plant and Microbial Biology, University of 
            California, Berkeley, 281 Koshland Hall, Berkeley, CA 94704, USA
REFERENCE   3  (bases 1 to 5444)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5444)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid"; 
            date: "2004"; volume: "51"; issue: "3"; pages: "238-245"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-NOV-2003) Plant and Microbial Biology, University of California,
            Berkeley, 281 Koshland Hall, Berkeley, CA 94704, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5444
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..495)
                     /note="3' end of dihydroorotate dehydrogenase (catalytic 
                     subunit) (pyrD) of Bacillus subtilis"
     CDS             1467..2258
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    complement(2714..3133)
                     /note="5' end of dihydroorotate dehydrogenase (pyrD) of
                     Bacillus subtilis"
     promoter        3538..3642
                     /label=AmpR promoter
     CDS             3643..4500
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4674..5262
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagagcgcg
       61 atgatgggca ggttgttttt catgattcca gctccttccg atgcattctt caattgattg
      121 atagccgtat tggagcaaaa cagatgggag ctgttcaata atctctggac atgcaaaagg
      181 attcacaaag tttgctgttc cgacagcgac tgcgcttgct cccgcgagaa gaaattccag
      241 ggcatcttca gccgtttgca cgcctcccat tccgataatc gggatgttga ccatctggct
      301 gacttcatac accatgcgaa tggcaaccgg cttcacagca gggcccgaaa gtccccctgt
      361 tttattcgct aatatcggtt tgccggtttt taaatcgagt ctcatgccga ttagtgtgtt
      421 gatcatcgta agaccgtccg ctcccgcttc ctcgatcgct aatgcaattt ctgtgatatt
      481 agccacgttc ggggcgcgcc aaactggaca catggaaaca cacaaattaa aaactggtct
      541 gatcgaaata gtacataatg gatttcctta cgcgaaatac gggcagacat ggcctgcccg
      601 gttattatta tttttgacac cagaccaact ggtaatggta gcgaccggcg ctcaggatcc
      661 tagaagctta tcgaattcga gctcggtacc cctggatttc actgatgaga atatcgtcgg
      721 agataaatat aataattcca cggactatag actatactag tatactccgt ctactgtacg
      781 atacacttcc gctcaggtcc ttgtccttta acgaggattg ttacgtacgc taatggcgtc
      841 aaaacaaaga ctctagacct aggccttaag atctgatcat atgcggtgtg aaataccgca
      901 cagatgcgta aggagaaaat accgcatcag gcgataaacc cagcgaacca tttgaggtga
      961 taggtaagat tataccgagg tatgaaaacg agaattggac ctttacagaa ttactctatg
     1021 aagcgccata tttaaaaagc taccaagacg aagaggatga agaggatgag gaggcagatt
     1081 gccttgaata tattgacaat actgataaga taatatatct tttatataga agatatcgcc
     1141 gtatgtaagg atttcagggg gcaaggcata ggcagcgcgc ttatcaatat atctatagaa
     1201 tgggcaaagc ataaaaactt gcatggacta atgcttgaaa cccaggacaa taaccttata
     1261 gcttgtaaat tctatcataa ttgtggtttc aaaatcggct ccgtcgatac tatgttatac
     1321 gccaactttc aaaacaactt tgaaaaagct gttttctggt atttaaggtt ttagaatgca
     1381 aggaacagtg aattggagtt cgtcttgtta taattagctt cttggggtat ctttaaatac
     1441 tgtagaaaag aggaaggaaa taataaatgg ctaaaatgag aatatcaccg gaattgaaaa
     1501 aactgatcga aaaataccgc tgcgtaaaag atacggaagg aatgtctcct gctaaggtat
     1561 ataagctggt gggagaaaat gaaaacctat atttaaaaat gacggacagc cggtataaag
     1621 ggaccaccta tgatgtggaa cgggaaaagg acatgatgct atggctggaa ggaaagctgc
     1681 ctgttccaaa ggtcctgcac tttgaacggc atgatggctg gagcaatctg ctcatgagtg
     1741 aggccgatgg cgtcctttgc tcggaagagt atgaagatga acaaagccct gaaaagatta
     1801 tcgagctgta tgcggagtgc atcaggctct ttcactccat cgacatatcg gattgtccct
     1861 atacgaatag cttagacagc cgcttagccg aattggatta cttactgaat aacgatctgg
     1921 ccgatgtgga ttgcgaaaac tgggaagaag acactccatt taaagatccg cgcgagctgt
     1981 atgatttttt aaagacggaa aagcccgaag aggaacttgt cttttcccac ggcgacctgg
     2041 gagacagcaa catctttgtg aaagatggca aagtaagtgg ctttattgat cttgggagaa
     2101 gcggcagggc ggacaagtgg tatgacattg ccttctgcgt ccggtcgatc agggaggata
     2161 tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag cctgattggg
     2221 agaaaataaa atattatatt ttactggatg aattgtttta gtacctagat ttagatgtct
     2281 aaaaagcttt aactacaagc tttttagaca tctaatcttt tctgaagtac atccgcaact
     2341 gtccatactc tgatgtttta tatcttttct aaaagttcgc tagatagggg tcccgagcgc
     2401 ctacgaggaa tttgtatcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     2461 cggtgcgggc ctcttcgcta ttacgccagg acctgcaggc atgcaccatt ccttgcggcg
     2521 gcggtgctct tcggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag
     2581 ggaggacgtc ggcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta
     2641 ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgacatgg atgagcgatg
     2701 atgatatccg tttaggcgcg ccaaaagcga ttccgcctgt tttcacattc gggcaggaaa
     2761 tattcaattc aagagcatga acattaggcg ctttgctgat atgttctgcg acttcaacat
     2821 aatcatcgac ttgagaacct gcgacattgg caatgatcgg tgtatcaaac tgctcaagcc
     2881 acggcagctc atgatgcaac acactatcca gccccggatt ttggagaccg atcgcattga
     2941 gcattccagc accagtctca gctacccgcg gcgtcggatt cccaaagcgc ggctcctttg
     3001 tcgtagcctt aatcatgata gctccaagac aagacaaatc ataaaaacgt gaaaattctt
     3061 ttccaaaacc gaagcagcct gatgcaggaa tgattgggtt tttcaaatca agtcccggca
     3121 atttcacctc tagcctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     3181 ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc
     3241 ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     3301 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     3361 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     3421 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
     3481 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     3541 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     3601 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     3661 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     3721 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     3781 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     3841 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     3901 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     3961 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     4021 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     4081 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     4141 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     4201 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     4261 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     4321 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     4381 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     4441 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     4501 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     4561 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     4621 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     4681 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     4741 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     4801 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     4861 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     4921 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     4981 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     5041 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     5101 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     5161 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     5221 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     5281 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     5341 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     5401 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aaga
//
