LOCUS       V003881                 4769 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003881
VERSION     V003881
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Middleton R, Hofmeister A.
  TITLE     New shuttle vectors for ectopic insertion of genes into Bacillus
            subtilis
  JOURNAL   Plasmid 51 (3), 238-245 (2004)
   PUBMED   15109830
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   Middleton RH, Hofmeister AEM.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2003) Plant and Microbial Biology, University of
            California, Berkeley, 281 Koshland Hall, Berkeley, CA 94704, USA
REFERENCE   3  (bases 1 to 4769)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4769)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid";
            date: "2004"; volume: "51"; issue: "3"; pages: "238-245"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (13-NOV-2003) Plant and Microbial Biology, University of California,
            Berkeley, 281 Koshland Hall, Berkeley, CA 94704, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4769
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..495)
                     /note="3' end of dihydroorotate dehydrogenase (catalytic
                     subunit) (pyrD) of Bacillus subtilis"
     CDS             complement(907..1554)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     misc_feature    complement(2039..2458)
                     /note="5' end of dihydroorotate dehydrogenase (pyrD) of
                     Bacillus subtilis"
     promoter        2863..2967
                     /label="AmpR promoter"
     CDS             2968..3825
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3999..4587
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagagcgcg
       61 atgatgggca ggttgttttt catgattcca gctccttccg atgcattctt caattgattg
      121 atagccgtat tggagcaaaa cagatgggag ctgttcaata atctctggac atgcaaaagg
      181 attcacaaag tttgctgttc cgacagcgac tgcgcttgct cccgcgagaa gaaattccag
      241 ggcatcttca gccgtttgca cgcctcccat tccgataatc gggatgttga ccatctggct
      301 gacttcatac accatgcgaa tggcaaccgg cttcacagca gggcccgaaa gtccccctgt
      361 tttattcgct aatatcggtt tgccggtttt taaatcgagt ctcatgccga ttagtgtgtt
      421 gatcatcgta agaccgtccg ctcccgcttc ctcgatcgct aatgcaattt ctgtgatatt
      481 agccacgttc ggggcgcgcc taaacggata tcatcatcgc tcatccatgt cgacgctctc
      541 ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg
      601 ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc
      661 ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt
      721 ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc
      781 cggccacgat gcgtccggcg tagaggatct ggagctgtaa tataaaaacc ttcttcaact
      841 aacggggcag gttagtgaca ttagaaaacc gactgtaaaa agtacagtcg gcattatctc
      901 atattataaa agccagtcat taggcctatc tgacaattcc tgaatagagt tcataaacaa
      961 tcctgcatga taaccatcac aaacagaatg atgtacctgt aaagatagcg gtaaatatat
     1021 tgaattacct ttattaatga attttcctgc tgtaataatg ggtagaaggt aattactatt
     1081 attattgata tttaagttaa acccagtaaa tgaagtccat ggaataatag aaagagaaaa
     1141 agcattttca ggtataggtg ttttgggaaa caatttcccc gaaccattat atttctctac
     1201 atcagaaagg tataaatcat aaaactcttt gaagtcattc tttacaggag tccaaatacc
     1261 agagaatgtt ttagatacac catcaaaaat tgtataaagt ggctctaact tatcccaata
     1321 acctaactct ccgtcgctat tgtaaccagt tctaaaagct gtatttgagt ttatcaccct
     1381 tgtcactaag aaaataaatg cagggtaaaa tttatatcct tcttgtttta tgtttcggta
     1441 taaaacacta atatcaattt ctgtggttat actaaaagtc gtttgttggt tcaaataatg
     1501 attaaatatc tcttttctct tccaattgtc taaatcaatt ttattaaagt tcatttgata
     1561 tgcctcctaa atttttatct aaagtgaatt taggaggctt acttgtctgc tttcttcatt
     1621 agaatcaatc cttttttaaa agtcaatatt actgtaacat aaatatatat tttaaaaata
     1681 tcccacttta tccaattttc gtttgttgaa ctaatgggtg ctttagttga agaataaaga
     1741 ccacattaaa aaatgtggtc ttttgtgttt ttttaaagga tttgagcgta gcgaaaaatc
     1801 cttttctttc ttatcttgat aataagggta actattgccg atgataagct gtcaaacatg
     1861 agaattcgat aagcttctag gatcctgagc gccggtcgct accattacca gttggtctgg
     1921 tgtcaaaaat aataataacc gggcaggcca tgtctgcccg tatttcgcgt aaggaaatcc
     1981 attatgtact atttcgatca gaccagtttt taatttgtgt gtttccatgt gtccagtttg
     2041 gcgcgccaaa agcgattccg cctgttttca cattcgggca ggaaatattc aattcaagag
     2101 catgaacatt aggcgctttg ctgatatgtt ctgcgacttc aacataatca tcgacttgag
     2161 aacctgcgac attggcaatg atcggtgtat caaactgctc aagccacggc agctcatgat
     2221 gcaacacact atccagcccc ggattttgga gaccgatcgc attgagcatt ccagcaccag
     2281 tctcagctac ccgcggcgtc ggattcccaa agcgcggctc ctttgtcgta gccttaatca
     2341 tgatagctcc aagacaagac aaatcataaa aacgtgaaaa ttcttttcca aaaccgaagc
     2401 agcctgatgc aggaatgatt gggtttttca aatcaagtcc cggcaatttc acctctagcc
     2461 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     2521 ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     2581 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     2641 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     2701 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     2761 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     2821 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     2881 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     2941 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     3001 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     3061 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     3121 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     3181 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     3241 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     3301 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     3361 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     3421 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     3481 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     3541 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     3601 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     3661 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     3721 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     3781 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     3841 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     3901 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     3961 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     4021 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     4081 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     4141 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     4201 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     4261 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     4321 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     4381 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     4441 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     4501 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     4561 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     4621 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     4681 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     4741 agcgagtcag tgagcgagga agcggaaga
//
