LOCUS       62056_18625        6346 bp DNA     circular SYN 12-JUN-2019
DEFINITION  Transformation vector pPtPDS1-M1, complete sequence.
ACCESSION   MK645852
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6346)
  AUTHORS   Taparia Y, Zarka A, Leu S, Zarivach R, Boussiba S, Khozin-Goldberg 
            I.
  TITLE     A novel endogenous selection marker for the diatom Phaeodactylum 
            tricornutum based on a unique mutation in phytoene desaturase 1
  JOURNAL   Sci Rep 9 (1), 8217 (2019)
  PUBMED    31160749
REFERENCE   2  (bases 1 to 6346)
  AUTHORS   Taparia Y, Leu S, Zarka A, Zarivach R, Khozin-Goldberg I, Boussiba 
            S.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2019) Albert Katz International School for Desert 
            Research, Ben-Gurion University of The Negev, French Associates 
            Institute for Agriculture and Biotechnology, Sede Boqer, Israel 
            8499000, Israel
REFERENCE   3  (bases 1 to 6346)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep"; 
            date: "2019"; volume: "9"; issue: "1"; pages: "8217"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-MAR-2019) Albert Katz International School for Desert Research, 
            Ben-Gurion University of The Negev, French Associates Institute for 
            Agriculture and Biotechnology, Sede Boqer, Israel 8499000, Israel"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6346
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      19..453
                     /regulatory_class="promoter"
     CDS             480..1196
                     /label=EGFP
                     /note="enhanced GFP"
     misc_feature    1213..1269
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     regulatory      1270..1598
                     /regulatory_class="terminator"
     regulatory      1605..1850
                     /regulatory_class="promoter"
     CDS             2117..2131
                     /label=SNAC tag
                     /note="tag for protein cleavage using Ni2+ ion (Dang et
                     al., 2019)"
     regulatory      3726..3967
                     /regulatory_class="terminator"
     promoter        complement(3988..4006)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(4264..4852)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5026..5883)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5884..5988)
                     /label=AmpR promoter
ORIGIN
        1 tatagaacca gatcccccgg gctgcaggac gcaatggagg attatcaccg caaaaatgaa
       61 cttcgaaaaa aactttcgag cgaccatgga aaaggaggat cagattcaga ttacaacagt
      121 ggattgctct ggtagcaaat atcttctgct agattggctc atggtcggtt ttggacgttc
      181 gaagctcacc gtcaaaagaa acaaaagaga agaatgacgt cttcgtgacg tagaatctac
      241 gactgtactc ggatctggga aatgaattga ctcacggtct tcttcgagtc ctgttacagg
      301 cccttggtcc gaacccccac acgatttttg caccaaagat ttgcttcaat ttgctggatg
      361 ttttgactgc aagatcagct ggcctagcaa gagtgctcgt gttgcttcgt cgggaatccc
      421 tacgaatttc agttctgcac aaatttgtct gccgtttcga gaattcgatc gtcaggccta
      481 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
      541 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
      601 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
      661 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
      721 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
      781 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
      841 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
      901 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
      961 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     1021 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     1081 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     1141 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaaa
     1201 gcgatcatcg actaattcga gctcggtacc cggggatcct ctagagtcga cctgcaggca
     1261 tgcaagcttc agaagcgtgc tatcgaactc aaccagggac gtgcggcaca aatgggcatc
     1321 cttgctctca tggtgcacga acagttggga gtctctatcc ttccttaaaa atttaatttt
     1381 cattagttgc agtcactccg ctttggtttc acagtcagga ataacactag ctcgtcttca
     1441 ccatggatgc caatctcgcc tattcatggt gtataaaagt tcaacatcca aagctagaac
     1501 ttttggaaag agaaagaata tccgaatagg gcacggcgtg ccgtattgtt ggagtggact
     1561 agcagaaagt gaggaaggca caggatgagt tttctcgaac tagtacatac cttcagcgtc
     1621 gtcttcactg tcacagtcaa ctgacagtaa tcgttgatcc ggagagattc aaaattcaat
     1681 ctgtttggac ctggataaga cacaagagcg acatcctgac atgaacgccg taaacagcaa
     1741 atcctggttg aacacgtatc cttttggggg cctcccgcta cgacgctcgc tccagctggg
     1801 gcttccttac tatacacagc gcgcatattt cacggttgcc agatgtcaag atgatgtttc
     1861 actataagac agggtcgtca tggttcctgc tgctgtccgc atccattacc acaaccctca
     1921 cgacgacaac gatcacaaca acccacgcct ttgccccgca cacgcgctta tccgtgcccc
     1981 acggtgcttc ccgactcgtc atgaaggatt tccccaaacc caatttggaa gatacggaca
     2041 attaccgttt gtatcgtgat ttgtcgcact ccttctccac gacactcaag gcaccgtcgc
     2101 cagaatcacg caagaaggta gccatcattg gcggtggctt gtccggtctc gcctgtgcca
     2161 agtacctcgc cgacgccgga caccaacccg tcgtctacga agcacgggac gtcctcggcg
     2221 gcaaggtctc cgcttggcaa gatgccgacg gagactggat cgaaaccgga ctacacattt
     2281 tctttggtgc ctacccgaat atgatgaatc tctttgccga acttgatatt cacgatcgtc
     2341 tccagtggaa ggtgcacaaa atgatctttg ccatgcagga actacccgga gaattcacaa
     2401 cctttgattt cattcccggc atacccgcac ccttcaattt cggactcgct attctcatga
     2461 accagaaaat gctcactttg ccggagaaaa tacagacggc tcccccgcta ctgcccatgc
     2521 tcgtacgcgg acaagacttt atcgacgaac aggacgaact ctccgtcctc gactttatgc
     2581 gcaagtacgg gatgcccgaa cgcatcaacg aagaagtctt tatcagcatg gccaaggcac
     2641 tcgatttcat tgatcccgac aaattgagta tgacggtcgt tttgaccgcc atgaatcgct
     2701 ttttgaacga agacaacgcc ctccaaatgg cctttctcga cggcaaccag cccgaccggc
     2761 tctgtgcacc catggtcgaa cacattcaag cccgtggcgg tcaagtcaac ctcaattccc
     2821 ccgtgcaaga aatcgtcacc cgcgaagacg gtagtgtcga ttacctcctg atgcggtccg
     2881 gagaaaaggt cgttgccgac gaatacgtgt ccgccatgcc cgtggatatt gtcaaacgca
     2941 tgttgcccga aaagtggcaa acaatgccct actttcgtca attcgacgaa ctcgaaggca
     3001 ttcccgtcat taacctccac atgtggttcg atcgcaaact caaagccgtc gatcacttgt
     3061 gcttttcccg ctcgccgctg ctgtccgttt acgccgacat gagcgtcacc tgcaaggaat
     3121 actacgacga atccgcttcc atgctcgagc tcgtctttgc cccgtgcagt ccactcgccg
     3181 gtggcaacgt gaattggatt gccaagacgg acgaagaaat catcgacgcg accatgggcg
     3241 aactggcccg actctttccc accgaaatcg ccgctgatcc cacctggccg gccaccaaga
     3301 accagggccc caacggtgag gccaaacttc gcaaatacgc cgtggtcaag gtgccccgct
     3361 cggtctacgc cgccattccc ggccgcaaca agtaccgccc gagtcaaacc actccaattg
     3421 acaatttcac gctggccggg gactggacgt cacaaaagtt tctgggatcc atggaaggcg
     3481 ccgtcctcgg gggcaagctc gccgccgaag tattggcccg caaggcggcc aacctacccg
     3541 cacccgaact cgccaacaaa cccgtgcggg atgaaattgt gcaaaaggcg caaacacacg
     3601 aagcgcgtcc accggccgga gtcaagggac aaggtgcgat tgcctttggc ggtggcgccg
     3661 tgctcgggac ggaaaacaaa gccttgcttc gggacgttga tcctagtcaa ttcgtggaag
     3721 cctagacctt ccttaaaaat ttaattttca ttagttgcag tcactccgct ttggtttcac
     3781 agtcaggaat aacactagct cgtcttcacc atggatgcca atctcgccta ttcatggtgt
     3841 ataaaagttc aacatccaaa gctagaactt ttggaaagag aaagaatatc cgaatagggc
     3901 acggcgtgcc gtattgttgg agtggactag cagaaagtga ggaaggcaca ggatgagttt
     3961 tctcgagact agttcgaggc cggtctccct atagtgagtc gtattaattt cgataagcca
     4021 ggttaacctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     4081 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     4141 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     4201 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     4261 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     4321 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     4381 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     4441 aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     4501 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     4561 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     4621 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     4681 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     4741 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     4801 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     4861 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     4921 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     4981 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     5041 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     5101 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     5161 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     5221 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     5281 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     5341 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     5401 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     5461 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     5521 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     5581 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     5641 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     5701 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     5761 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     5821 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     5881 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     5941 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     6001 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     6061 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     6121 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     6181 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
     6241 agagcagatt gtactgagag tgcaccatat ggacatattg tcgttagaac gcggctacaa
     6301 ttaatacata accttatgta tcatacacat acgatttagg tgacac
//
