LOCUS       62056_18620       12394 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12394)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..12394
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(788..1156)
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(1189..1298)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     promoter        1615..1633
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             2254..2625
                     /codon_start=1
                     /label=BleoR
                     /note="antibiotic-binding protein"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    3125..3627
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     promoter        3628..3814
                     /label=HIS3 promoter
     CDS             3815..4471
                     /codon_start=1
                     /label=HIS3
                     /note="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVL"
     CDS             5052..9152
                     /codon_start=1
                     /label=Cas9
                     /note="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     misc_RNA        9736..9811
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(10203..10221)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(10290..10320)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(10335..10356)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(10644..11232)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11406..12263)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(12264..12368)
                     /label=AmpR promoter
ORIGIN
        1 ggaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg cgccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctgat
      661 cgtcttgcct tgctcgtcgg tgatgtactt caccagctcc gcgaagtcgc tcttcttgat
      721 ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc cggccgtttt agcggctaaa
      781 aaagtcatgg ctctgccctc gggcggacca cgcccatcat gaccttgcca agctcgtcct
      841 gcttctcttc gatcttcgcc agcagggcga ggatcgtggc atcaccgaac cgcgccgtgc
      901 gcgggtcgtc ggtgagccag agtttcagca ggccgcccag gcggcccagg tcgccattga
      961 tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc cgtgattttg tagccctggc
     1021 cgacggccag caggtaggcc gacaggctca tgccggccgc cgccgccttt tcctcaatcg
     1081 ctcttcgttc gtctggaagg cagtacacct tgataggtgg gctgcccttc ctggttggct
     1141 tggtttcatc agccatccgc ttgccctcat ctgttacgcc ggcggtagcc ggccagcctc
     1201 gcagagcagg attcccgttg agcaccgcca ggtgcgaata agggacagtg aagaaggaac
     1261 acccgctcgc gggtgggcct acttcaccta tcctgcccgg ctgacgccgt tggatacacc
     1321 aaggaaagtc tacacgaacc ctttggcaaa atcctgtata tcgtgcgaaa aaggatggat
     1381 ataccgaaaa aatcgctata atgaccccga agcagggtta tgcagcggaa gatgccattc
     1441 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     1501 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     1561 ccagtcacga cgttgtaaaa cgacggccag tgaccatgat tacgccaagc tcgaaattaa
     1621 ccctcactaa agggaacaaa agctggtacc taaccctgcg atagaccttt tccaaactca
     1681 cgcagtccaa gaaaacaaag gggtgagaag tatacgcacc tttcggtttc ggcataattc
     1741 ttaaactctt gtggtcactt tcttgtgaag aagctagggg cactcgtttt ccctcagagc
     1801 ctgcaaacac aaaattcctg cagtcaattg tcccaacact cggcaagccg tatgcgcaag
     1861 caacgatgcg cagaaggccg tggatggatg gcgactcgcg atatggcttc ttgggtcgcc
     1921 agtgtggtat gtccggcgta tgtcaatacg cgaattcgga cgactggcat ctctaggagg
     1981 aggattcctt cttttatgac atgtttattt tatatacatt gatgctttcc gacagtcgga
     2041 agtaataaat gaatttattt caagactacc tatactcctt tgacttgttc gactaatctt
     2101 accgcttact aaaatctcga aatcacgctt gacctctcgc acgcaaattt ttgctgctgg
     2161 acgctacgca ctcggcccaa ttcttctcgg tcctcgtcgt cgcaattgtc gttgcgttga
     2221 tcttgcaccg aaggaatcag agaatagaat accatggcca agttgaccag tgccgttccg
     2281 gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc
     2341 tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc
     2401 atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc
     2461 ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc
     2521 tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc
     2581 gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaag ataagaatat
     2641 ctcattgtga acatctatga tttaccaatt ttattctttg tttacagtta gacgccagta
     2701 attgtgctgt ttctctcaag tctgtgtcaa tacaaactac gaaacttggc aatttttctc
     2761 ttgaatatga gcacgagatg aaacgcacaa aggaaattag tttccatcct ttgacaaagt
     2821 ttgttgctgt ttagagaaca gatgtcaaaa ttaacgtgcc atggaattga accatggtgc
     2881 gctatcccca aatcacgtcg tttgacctcg tcacatttaa ggtatatcaa gcatattcac
     2941 ttatatcttg aatcctccct gcttgatatt cctttgccct tgagccatct tccccacacg
     3001 ctggagatga cccctgcccc tcctttcttt atccacccga gtaaggtgtc gacaagtcac
     3061 tttgcccctg agccatcctc cccactcagg agatgacccc tacccctttc ttctttatcc
     3121 acccccacgt gctataaaaa taattataat ttaaattttt taatataaat atataaatta
     3181 aaaatagaaa gtaaaaaaag aaattaaaga aaaaatagtt tttgttttcc gaagatgtaa
     3241 aagactctag ggggatcgcc aacaaatact accttttacc ttgctcttcc tgctctcagg
     3301 tattaatgcc gaattgtttc atcttgtctg tgtagaagac cacacacgaa aatcctgtga
     3361 ttttacattt tacttatcgt taatcgaatg tatatctatt taatctgctt ttcttgtcta
     3421 ataaatatat atgtaaagta cgctttttgt tgaaattttt taaacctttg tttatttttt
     3481 tttcttcatt ccgtaactct tctaccttct ttatttactt tctaaaatcc aaatacaaaa
     3541 cataaaaata aataaacaca gagtaaattc ccaaattatt ccatcattaa aagatacgag
     3601 gcgcgtgtaa gttacaggca agcgatccta gtacactcta tattttttta tgcctcggta
     3661 atgattttca tttttttttt ccacctagcg gatgactctt tttttttctt agcgattggc
     3721 attatcacat aatgaattat acattatata aagtaatgtg atttcttcga agaatatact
     3781 aaaaaatgag caggcaagat aaacgaaggc aaagatgaca gagcagaaag ccctagtaaa
     3841 gcgtattaca aatgaaacca agattcagat tgcgatctct ttaaagggtg gtcccctagc
     3901 gatagagcac tcgatcttcc cagaaaaaga ggcagaagca gtagcagaac aggccacaca
     3961 atcgcaagtg attaacgtcc acacaggtat agggtttctg gaccatatga tacatgctct
     4021 ggccaagcat tccggctggt cgctaatcgt tgagtgcatt ggtgacttac acatagacga
     4081 ccatcacacc actgaagact gcgggattgc tctcggtcaa gcttttaaag aggccctagg
     4141 ggccgtgcgt ggagtaaaaa ggtttggatc aggatttgcg cctttggatg aggcactttc
     4201 cagagcggtg gtagatcttt cgaacaggcc gtacgcagtt gtcgaacttg gtttgcaaag
     4261 ggagaaagta ggagatctct cttgcgagat gatcccgcat tttcttgaaa gctttgcaga
     4321 ggctagcaga attaccctcc acgttgattg tctgcgaggc aagaatgatc atcaccgtag
     4381 tgagagtgcg ttcaaggctc ttgcggttgc cataagagaa gccacctcgc ccaatggtac
     4441 caacgatgtt ccctccacca aaggtgttct ttatgcaatc tcgcctattc atggtgtata
     4501 aaagttcaac atccaaagct agaacttttg gaaagagaaa gaatatccga atagggcacg
     4561 gcgtgccgta ttgttggagt ggactagcag aaagtgagga aggcacagga tgagttttct
     4621 cgagacacat agcttcagcg tcgtgtaggc taggcagagg tgagttttct cgagacatac
     4681 cttcagcgtc gtcttcactg tcacagtcaa ctgacagtaa tcgttgatcc ggagagattc
     4741 aaaattcaat ctgtttggac ctggataaga cacaagagcg acatcctgac atgaacgccg
     4801 taaacagcaa atcctggttg aacacgtatc cttttggggg cctccgctac gacgctcgct
     4861 ccagctgggg cttccttact atacacagcg cgcatatttc acggttgcca gaagtcaaga
     4921 tggcctctag aatggactat aaggaccacg acggagatta caaggatcat gatattgatt
     4981 acaaagacga tgatgataaa atggccccaa agaagaagcg gaaagtcggt atacacggag
     5041 tcccagcagc cgacaagaag tactcgattg gattggacat cggcaccaac tctgttggat
     5101 gggccgtcat cacggacgag tacaaggtgc ccagcaagaa attcaaagta ctgggaaaca
     5161 ctgaccgcca ttcgatcaag aagaatttga ttggagcttt gctgtttgac agcggcgaaa
     5221 cagcggaagc cacgcggctt aaacgaaccg cgagacgaag atatacgcga cggaagaacc
     5281 gtatctgcta tctacaagag attttctcga atgagatggc aaaagtcgat gactctttct
     5341 ttcaccgatt ggaagaatcc ttcctcgttg aagaggataa gaaacatgag cggcacccga
     5401 tatttggaaa cattgtggac gaggtggctt accatgagaa atacccgact atttaccacc
     5461 tgcgaaagaa attggtggac tcgacggaca aagcggatct tcgcctaatc tatttggcgc
     5521 tagcacatat gattaagttt cgcggccact tcttgatcga aggcgacttg aatccggaca
     5581 acagtgatgt tgacaaactg tttattcaac ttgtacaaac ttacaaccag ctctttgaag
     5641 aaaatcccat taacgcttcg ggagtggacg cgaaagccat cctttctgcc cgactcagca
     5701 agagccgacg cctagaaaat ttgattgcgc aacttcccgg cgagaaaaag aatggacttt
     5761 ttggaaatct tattgctttg tcgctgggcc ttacgccgaa ctttaagagc aacttcgacc
     5821 ttgcggaaga tgccaaattg caactgagta aagacacata tgacgacgac ctcgacaatc
     5881 ttttggcaca aataggagat cagtacgccg acctcttttt ggcggctaag aacttgtccg
     5941 acgccattct gttgtcggac atccttcgag ttaatacgga gattactaag gcgcccttga
     6001 gcgcctctat gatcaaaaga tacgacgagc atcaccaaga cctgaccctc cttaaagctc
     6061 tcgtccggca acagctgcct gagaagtaca aagagatttt ctttgatcaa agcaaaaacg
     6121 gatacgcggg ctacattgac gggggagcct cgcaagaaga gttctacaag tttattaaac
     6181 cgatcctgga aaagatggac ggcacggagg aattactcgt gaaacttaac cgagaggacc
     6241 ttttgcgtaa gcaacggact ttcgacaacg gatctattcc gcaccaaatc catctcggag
     6301 aattgcacgc aattctgcgc cggcaagaag atttttatcc atttctcaag gacaaccggg
     6361 aaaagattga gaaaatctta accttccgca taccctacta cgtcggccct ttggccaggg
     6421 gaaattcgag atttgcctgg atgacgcgaa agagtgagga aactattact ccctggaatt
     6481 tcgaagaagt ggtagacaaa ggagcttccg cgcaatcctt tatcgagcgc atgaccaact
     6541 tcgataagaa cctcccgaat gagaaagtcc tacccaagca ttcgttgctc tacgaatact
     6601 ttacggtgta taatgagctg actaaagtca aatacgttac agagggaatg cgaaaaccgg
     6661 ctttcctctc gggagagcag aaaaaggcca tagtcgatct tctctttaaa acgaaccgta
     6721 aagttaccgt gaaacaacta aaagaggact acttcaagaa aatcgaatgc tttgactccg
     6781 ttgaaatttc cggcgttgaa gatcggttca atgcctcctt gggaacatac cacgatctcc
     6841 tgaaaattat caaagacaag gactttcttg acaatgagga aaacgaggac attcttgaag
     6901 atattgtctt gacgctaaca ctttttgagg atcgagagat gattgaggaa cgccttaaaa
     6961 cctatgcgca tctgttcgac gacaaagtta tgaagcaatt gaagcgccgg agatatacgg
     7021 gatggggaag gttgagtcgg aaactgataa atggcatacg cgataagcag tccggcaaaa
     7081 caattctcga tttcttgaag tccgacggat ttgcgaatcg aaacttcatg caactgatcc
     7141 atgacgattc gttgaccttt aaagaggaca ttcaaaaagc ccaagtatcc ggacaaggag
     7201 attctttgca cgagcatatt gcgaatcttg ctggaagtcc ggctattaaa aagggcattc
     7261 tccagacagt aaaggtcgtg gatgaactcg ttaaagttat gggccgacac aaacccgaga
     7321 acatagtcat cgaaatggcg agagagaacc agaccaccca aaagggacag aaaaattcac
     7381 gcgagcgaat gaagcggatt gaagagggga tcaaagagct tggcagccaa attctgaaag
     7441 aacacccggt tgaaaacacc caattgcaga acgagaagct ctacctgtac tatcttcaaa
     7501 atgggcgtga tatgtacgtc gatcaagaat tggacatcaa ccggctatcc gattacgatg
     7561 tggaccatat tgttcctcag tcgtttctca aagacgactc catagacaat aaggttctaa
     7621 cgagaagcga caagaaccgc ggcaaatcag acaacgtccc ctccgaggaa gtagttaaga
     7681 aaatgaagaa ttactggcgc caattgctga acgctaagct cattacccaa agaaagtttg
     7741 acaatttgac gaaagccgag cgaggcgggc tctcggaact ggataaggcc ggcttcatta
     7801 aaagacaatt ggtggaaacg cgacaaatca caaagcacgt cgcacagatt ctcgactccc
     7861 gcatgaacac taagtatgac gagaatgaca aactaatccg ggaagtcaaa gtgattacct
     7921 tgaagtccaa gctggtttcc gattttcgta aagattttca gttttacaaa gttcgcgaga
     7981 tcaacaacta ccatcacgcg cacgacgctt acttgaacgc tgtcgtggga acggccttga
     8041 ttaaaaaata ccctaagctg gaaagcgagt tcgtttacgg cgattacaag gtgtatgacg
     8101 tacgcaaaat gatcgcgaag tcagagcagg aaattggcaa agctactgcg aagtacttct
     8161 tttatagcaa cataatgaat tttttcaaaa cggagattac cctcgctaac ggcgagattc
     8221 gcaagcgccc tcttatcgag acaaacggcg aaacggggga gattgtctgg gataaaggcc
     8281 gcgattttgc gactgtgcgg aaagtcttgt cgatgccgca agtgaatata gtcaaaaaga
     8341 cggaggtgca aacaggcggc ttttcgaaag aatctatcct tcccaaaagg aactctgata
     8401 agttgattgc gcgaaaaaag gattgggacc ctaagaagta tggcggcttt gacagcccga
     8461 ccgtagcata ttctgtcctt gtggtggcca aagtcgaaaa gggcaaatcc aagaaactga
     8521 agagtgtgaa agaattgctt gggattacga tcatggaaag atcatctttt gagaagaatc
     8581 ccatcgactt tctcgaagct aaaggctaca aagaagtgaa aaaggacctg attatcaagt
     8641 tgcctaagta ctccttgttt gagctggaaa atggccgtaa gcgaatgctc gcttctgccg
     8701 gcgaactcca gaagggaaac gaattggcgc tgccctccaa atatgttaac tttctttacc
     8761 ttgcctcgca ctatgagaag ctcaagggct cccccgagga taatgagcaa aaacaactgt
     8821 ttgtagaaca gcataagcac tacttggacg agatcattga gcaaattagc gagttctcca
     8881 agcgagtcat ccttgccgac gctaatctgg acaaagtcct gtccgcctac aataagcacc
     8941 gcgataagcc aatccgagag caagcggaga atattatcca cttgtttacg ctcaccaatc
     9001 ttggagcccc tgccgccttt aagtactttg acaccaccat cgaccgcaag aggtacacca
     9061 gcaccaaaga agtcttggac gccaccctca ttcaccaatc aatcaccggc ttgtacgaga
     9121 cacgcatcga cctgtctcaa ctcggaggcg acaaaaggcc ggcggccacg aaaaaggccg
     9181 gccaggcaaa aaagaaaaag taatctggat ccctacctcg actttggctg ggacactttc
     9241 agtgaggaca agaagctcca gaagcgtgct atcgaactca accagggacg tgcggcacaa
     9301 atgggcatcc ttgctctcat ggtgcacgaa cagttgggag tctctatcct tccttaaaaa
     9361 tttaattttc attagttgca gtcactccgc tttggtttca cagtcaggaa taacactagc
     9421 tcgtcttcac tgcaggttgg ctcggaagtt ggtgttgacg gtgagctgga aattggttgt
     9481 cggtcactgc tagcgagaag aaaacggagg acagaaggaa gtgaaactcg gttcgttctc
     9541 gacagcctca ctgtcaatat gctcattttc aatccttagc gcttttaatg tcgaattgac
     9601 ggtaaattga ataggatcta taatatctac aaggtacttt gacacgccaa gtattcattg
     9661 ttagtcaaca atattttaga gctttataag gtcaaaaaac accttcaaag tcgaggagac
     9721 cgagagaggg tctcagtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta
     9781 tcaacttgaa aaagtggcac cgagtcggtg ctttttttta gaaccgctca cccatgctat
     9841 cgtatgtcat ttacattgac aaactgctat gaatgcgttt gcgataactt cagctcgctg
     9901 aagccctaga catttcgttt gactaaattg ttttccggtt gctttactac cgaccggtct
     9961 gcgggtagca tgtcttggct tttgcgagtt cgagagaatt tatggacaag tgcgtttaca
    10021 gtttggaaca cgagtgtgag tgagcggaat tggctatcta taacattctt atttcaggag
    10081 gctggtgatt agcagaattt tcaaagatct cgaatctaaa atcgccatgg gctcgacgaa
    10141 cttttctgca agcttatcga taccgtcgac ctcgaggggg ggcccggtac cagcttttgt
    10201 tccctttagt gagggttaat tccgagcttg gcgtaatcat ggtcatagct gtttcctgtg
    10261 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
    10321 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
    10381 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
    10441 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
    10501 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
    10561 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
    10621 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
    10681 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
    10741 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
    10801 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
    10861 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
    10921 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
    10981 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
    11041 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
    11101 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
    11161 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
    11221 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
    11281 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
    11341 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
    11401 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
    11461 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
    11521 cccagtgctg caatgatacc gcgagagcca cgctcaccgg ctccagattt atcagcaata
    11581 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
    11641 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
    11701 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
    11761 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
    11821 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
    11881 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
    11941 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
    12001 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
    12061 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
    12121 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
    12181 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
    12241 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
    12301 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    12361 gttccgcgca catttccccg aaaagtgcca cctg
//
