LOCUS       40924_35444        3548 bp DNA     circular SYN 18-DEC-2018
DEFINITION  E.coli-K.pastoris shuttle vector pPpT4_S, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Naatsaari L, Mistlberger B, Ruth C, Hajek T, Hartner FS, Glieder A.
  TITLE     Deletion of the Pichia pastoris KU70 Homologue Facilitates Platform 
            Strain Generation for Gene Expression and Synthetic Biology
  JOURNAL   PLoS ONE 7 (6), E39720 (2012)
  PUBMED    22768112
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Naeaetsaari L, Mistlberger B, Ruth C, Hajek T, Hartner F, Glieder A.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2012) Institute of Molecular Biotechnology, Graz 
            University of Technology, Petersgasse 14/2, Graz, Styria 8010, 
            Austria
REFERENCE   3  (bases 1 to 3548)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3548)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2012"; volume: "7"; issue: "6"; pages: "E39720"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-JAN-2012) Institute of Molecular Biotechnology, Graz University 
            of Technology, Petersgasse 14/2, Graz, Styria 8010, Austria"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: SeqMan v. 7.0.0
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        139..1071
                     /label=AOX1 promoter
                     /note="inducible promoter, regulated by methanol"
     terminator      1100..1346
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     regulatory      1361..1918
                     /label=ILV5 promoter
                     /note="ILV5 promoter"
                     /regulatory_class="promoter"
     regulatory      1919..1982
                     /label=EM72 synthetic promoter
                     /note="EM72 synthetic promoter"
                     /regulatory_class="promoter"
     CDS             1984..2355
                     /label=BleoR
                     /note="antibiotic-binding protein"
     regulatory      2360..2835
                     /label=AOD terminator
                     /note="AOD terminator"
                     /regulatory_class="terminator"
     rep_origin      2882..3470
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatccaatt cccgctttga ctgcctgaaa tctccatcgc ctacaatgat gacatttgga
       61 tttggttgac tcatgttggt attgtgaaat agacgcagat cgggaacact gaaaaataca
      121 cagttattat tcatttaaat aacatccaaa gacgaaaggt tgaatgaaac ctttttgcca
      181 tccgacatcc acaggtccat tctcacacat aagtgccaaa cgcaacagga ggggatacac
      241 tagcagcaga ccgttgcaaa cgcaggacct ccactcctct tctcctcaac acccactttt
      301 gccatcgaaa aaccagccca gttattgggc ttgattggag ctcgctcatt ccaattcctt
      361 ctattaggct actaacacca tgactttatt agcctgtcta tcctggcccc cctggcgagg
      421 ttcatgtttg tttatttccg aatgcaacaa gctccgcatt acacccgaac atcactccag
      481 atgagggctt tctgagtgtg gggtcaaata gtttcatgtt ccccaaatgg cccaaaactg
      541 acagtttaaa cgctgtcttg gaacctaata tgacaaaagc gtgatctcat ccaagatgaa
      601 ctaagtttgg ttcgttgaaa tgctaacggc cagttggtca aaaagaaact tccaaaagtc
      661 ggcataccgt ttgtcttgtt tggtattgat tgacgaatgc tcaaaaataa tctcattaat
      721 gcttagcgca gtctctctat cgcttctgaa ccccggtgca cctgtgccga aacgcaaatg
      781 gggaaacacc cgctttttgg atgattatgc attgtctcca cattgtatgc ttccaagatt
      841 ctggtgggaa tactgctgat agcctaacgt tcatgatcaa aatttaactg ttctaacccc
      901 tacttgacag caatatataa acagaaggaa gctgccctgt cttaaacctt tttttttatc
      961 atcattatta gcttactttc ataattgcga ctggttccaa ttgacaagct tttgatttta
     1021 acgactttta acgacaactt gagaagatca aaaaacaact aattattgaa agaattcact
     1081 agtggcgcgc cgcggccgct caagaggatg tcagaatgcc atttgcctga gagatgcagg
     1141 cttcattttt gatacttttt tatttgtaac ctatatagta taggattttt tttgtcattt
     1201 tgtttcttct cgtacgagct tgctcctgat cagcctatct cgcagcagat gaatatcttg
     1261 tggtaggggt ttgggaaaat cattcgagtt tgatgttttt cttggtattt cccactcctc
     1321 ttcagagtac agaagattaa gtgagacctt cgtttgtgcg gatccttcag taatgtcttg
     1381 tttcttttgt tgcagtggtg agccattttg acttcgtgaa agtttcttta gaatagttgt
     1441 ttccagaggc caaacattcc acccgtagta aagtgcaagc gtaggaagac caagactggc
     1501 ataaatcagg tataagtgtc gagcactggc aggtgatctt ctgaaagttt ctactagcag
     1561 ataagatcca gtagtcatgc atatggcaac aatgtaccgt gtggatctaa gaacgcgtcc
     1621 tactaacctt cgcattcgtt ggtccagttt gttgttatcg atcaacgtga caaggttgtc
     1681 gattccgcgt aagcatgcat acccaaggac gcctgttgca attccaagtg agccagttcc
     1741 aacaatcttt gtaatattag agcacttcat tgtgttgcgc ttgaaagtaa aatgcgaaca
     1801 aattaagaga taatctcgaa accgcgactt caaacgccaa tatgatgtgc ggcacacaat
     1861 aagcgttcat atccgctggg tgactttctc gctttaaaaa attatccgaa aaaattttct
     1921 agagtgttgt tactttatac ttccggctcg tataatacga caaggtgtaa ggaggactaa
     1981 accatggcta aactcacctc tgctgttcca gtcctgactg ctcgtgatgt tgctggtgct
     2041 gttgagttct ggactgatag actcggtttc tcccgtgact tcgtagagga cgactttgcc
     2101 ggtgttgtac gtgacgacgt taccctgttc atctccgcag ttcaggacca ggttgtgcca
     2161 gacaacactc tggcatgggt atgggttcgt ggtctggacg aactgtacgc tgagtggtct
     2221 gaggtcgtgt ctaccaactt ccgtgatgca tctggtccag ctatgaccga gatcggtgaa
     2281 cagccctggg gtcgtgagtt tgcactgcgt gatccagctg gtaactgcgt gcatttcgtc
     2341 gcagaagagc aggactaaca attgacacct tacgattatt tagagagtat ttattagttt
     2401 tattgtatgt atacggatgt tttattatct atttatgccc ttatattctg taactatcca
     2461 aaagtcctat cttatcaagc cagcaatcta tgtccgcgaa cgtcaactaa aaataagctt
     2521 tttatgctct tctctctttt tttcccttcg gtataattat accttgcatc cacagattct
     2581 cctgccaaat tttgcataat cctttacaac atggctatat gggagcactt agcgccctcc
     2641 aaaacccata ttgcctacgc atgtataggt gttttttcca caatattttc tctgtgctct
     2701 ctttttatta aagagaagct ctatatcgga gaagcttctg tggccgttat attcggcctt
     2761 atcgtgggac cacattgcct gaattggttt gccccggaag attggggaaa cttggatctg
     2821 attaccttag ctgcaggtac cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     2881 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     2941 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     3001 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     3061 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     3121 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     3181 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     3241 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     3301 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     3361 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     3421 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     3481 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     3541 cggtaccc
//
