LOCUS       40924_35434        3103 bp DNA     circular SYN 18-DEC-2018
DEFINITION  E.coli-K.pastoris shuttle vector pPpT4GAP_S, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Naatsaari L, Mistlberger B, Ruth C, Hajek T, Hartner FS, Glieder A.
  TITLE     Deletion of the Pichia pastoris KU70 Homologue Facilitates Platform 
            Strain Generation for Gene Expression and Synthetic Biology
  JOURNAL   PLoS ONE 7 (6), E39720 (2012)
  PUBMED    22768112
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Naeaetsaari L, Mistlberger B, Ruth C, Hajek T, Hartner F, Glieder A.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2012) Institute of Molecular Biotechnology, Graz 
            University of Technology, Petersgasse 14/2, Graz, Styria 8010, 
            Austria
REFERENCE   3  (bases 1 to 3103)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3103)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2012"; volume: "7"; issue: "6"; pages: "E39720"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-JAN-2012) Institute of Molecular Biotechnology, Graz University 
            of Technology, Petersgasse 14/2, Graz, Styria 8010, Austria"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: SeqMan v. 7.0.0
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        141..617
                     /label=GAP promoter
                     /note="promoter for Pichia pastoris
                     glyceraldehyde-3-phosphate dehydrogenase"
     terminator      655..901
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     regulatory      916..1473
                     /label=ILV5 promoter
                     /note="ILV5 promoter"
                     /regulatory_class="promoter"
     regulatory      1474..1537
                     /label=EM72 synthetic promoter
                     /note="EM72 synthetic promoter"
                     /regulatory_class="promoter"
     CDS             1539..1910
                     /label=BleoR
                     /note="antibiotic-binding protein"
     regulatory      1915..2390
                     /label=AOD terminator
                     /note="AOD terminator"
                     /regulatory_class="terminator"
     rep_origin      2437..3025
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatccaatt cccgctttga ctgcctgaaa tctccatcgc ctacaatgat gacatttgga
       61 tttggttgac tcatgttggt attgtgaaat agacgcagat cgggaacact gaaaaataca
      121 cagttattat tcatttaaat tttttgtaga aatgtcttgg tgtcctcgtc caatcaggta
      181 gccatctctg aaatatctgg ctccgttgca actccgaacg acctgctggc aacgtaaaat
      241 tctccggggt aaaacttaaa tgtggagtaa tggaaccaga aacgtctctt cccttctctc
      301 tccttccacc gcccgttacc gtccctagga aattttactc tgctggagag cttcttctac
      361 ggcccccttg cagcaatgct cttcccagca ttacgttgcg ggtaaaacgg aggtcgtgta
      421 cccgacctag cagcccaggg atggaaaagt cccggccgtc gctggcaata atagcgggcg
      481 gacgcatgtc atgagattat tggaaaccac cagaatcgaa tataaaaggc gaacaccttt
      541 cccaattttg gtttctcctg acccaaagac tttaaattta atttatttgt ccctatttca
      601 atcaattgaa caactatcaa aacacagaat tcactagtgg cgcgccgcgg ccgctcaaga
      661 ggatgtcaga atgccatttg cctgagagat gcaggcttca tttttgatac ttttttattt
      721 gtaacctata tagtatagga ttttttttgt cattttgttt cttctcgtac gagcttgctc
      781 ctgatcagcc tatctcgcag cagatgaata tcttgtggta ggggtttggg aaaatcattc
      841 gagtttgatg tttttcttgg tatttcccac tcctcttcag agtacagaag attaagtgag
      901 accttcgttt gtgcggatcc ttcagtaatg tcttgtttct tttgttgcag tggtgagcca
      961 ttttgacttc gtgaaagttt ctttagaata gttgtttcca gaggccaaac attccacccg
     1021 tagtaaagtg caagcgtagg aagaccaaga ctggcataaa tcaggtataa gtgtcgagca
     1081 ctggcaggtg atcttctgaa agtttctact agcagataag atccagtagt catgcatatg
     1141 gcaacaatgt accgtgtgga tctaagaacg cgtcctacta accttcgcat tcgttggtcc
     1201 agtttgttgt tatcgatcaa cgtgacaagg ttgtcgattc cgcgtaagca tgcataccca
     1261 aggacgcctg ttgcaattcc aagtgagcca gttccaacaa tctttgtaat attagagcac
     1321 ttcattgtgt tgcgcttgaa agtaaaatgc gaacaaatta agagataatc tcgaaaccgc
     1381 gacttcaaac gccaatatga tgtgcggcac acaataagcg ttcatatccg ctgggtgact
     1441 ttctcgcttt aaaaaattat ccgaaaaaat tttctagagt gttgttactt tatacttccg
     1501 gctcgtataa tacgacaagg tgtaaggagg actaaaccat ggctaaactc acctctgctg
     1561 ttccagtcct gactgctcgt gatgttgctg gtgctgttga gttctggact gatagactcg
     1621 gtttctcccg tgacttcgta gaggacgact ttgccggtgt tgtacgtgac gacgttaccc
     1681 tgttcatctc cgcagttcag gaccaggttg tgccagacaa cactctggca tgggtatggg
     1741 ttcgtggtct ggacgaactg tacgctgagt ggtctgaggt cgtgtctacc aacttccgtg
     1801 atgcatctgg tccagctatg accgagatcg gtgaacagcc ctggggtcgt gagtttgcac
     1861 tgcgtgatcc agctggtaac tgcgtgcatt tcgtcgcaga agagcaggac taacaattga
     1921 caccttacga ttatttagag agtatttatt agttttattg tatgtatacg gatgttttat
     1981 tatctattta tgcccttata ttctgtaact atccaaaagt cctatcttat caagccagca
     2041 atctatgtcc gcgaacgtca actaaaaata agctttttat gctcttctct ctttttttcc
     2101 cttcggtata attatacctt gcatccacag attctcctgc caaattttgc ataatccttt
     2161 acaacatggc tatatgggag cacttagcgc cctccaaaac ccatattgcc tacgcatgta
     2221 taggtgtttt ttccacaata ttttctctgt gctctctttt tattaaagag aagctctata
     2281 tcggagaagc ttctgtggcc gttatattcg gccttatcgt gggaccacat tgcctgaatt
     2341 ggtttgcccc ggaagattgg ggaaacttgg atctgattac cttagctgca ggtaccactg
     2401 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     2461 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgtcggatca
     2521 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     2581 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     2641 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     2701 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     2761 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     2821 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     2881 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     2941 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     3001 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     3061 cttttgctgg ccttttgctc acatgttctt tcctgcggta ccc
//
