LOCUS       40924_35429        3546 bp DNA     circular SYN 18-DEC-2018
DEFINITION  E.coli-K.pastoris shuttle vector pPpT4, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Naatsaari L, Mistlberger B, Ruth C, Hajek T, Hartner FS, Glieder A.
  TITLE     Deletion of the Pichia pastoris KU70 Homologue Facilitates Platform 
            Strain Generation for Gene Expression and Synthetic Biology
  JOURNAL   PLoS ONE 7 (6), E39720 (2012)
  PUBMED    22768112
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Naeaetsaari L, Mistlberger B, Ruth C, Hajek T, Hartner F, Glieder A.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2012) Institute of Molecular Biotechnology, Graz 
            University of Technology, Petersgasse 14/2, Graz, Styria 8010, 
            Austria
REFERENCE   3  (bases 1 to 3546)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3546)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2012"; volume: "7"; issue: "6"; pages: "E39720"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-JAN-2012) Institute of Molecular Biotechnology, Graz University 
            of Technology, Petersgasse 14/2, Graz, Styria 8010, Austria"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: SeqMan v. 7.0.0
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        134..1069
                     /label=AOX1 promoter
                     /note="inducible promoter, regulated by methanol"
     terminator      1098..1344
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     regulatory      1359..1916
                     /label=ILV5 promoter
                     /note="ILV5 promoter"
                     /regulatory_class="promoter"
     regulatory      1917..1980
                     /label=EM72 synthetic promoter
                     /note="EM72 synthetic promoter"
                     /regulatory_class="promoter"
     CDS             1982..2353
                     /label=BleoR
                     /note="antibiotic-binding protein"
     regulatory      2358..2833
                     /label=AOD terminator
                     /note="AOD terminator"
                     /regulatory_class="terminator"
     rep_origin      2880..3468
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatccaatt cccgctttga ctgcctgaaa tctccatcgc ctacaatgat gacatttgga
       61 tttggttgac tcatgttggt attgtgaaat agacgcagat cgggaacact gaaaaataca
      121 cagttattat tcagatctaa catccaaaga cgaaaggttg aatgaaacct ttttgccatc
      181 cgacatccac aggtccattc tcacacataa gtgccaaacg caacaggagg ggatacacta
      241 gcagcagacc gttgcaaacg caggacctcc actcctcttc tcctcaacac ccacttttgc
      301 catcgaaaaa ccagcccagt tattgggctt gattggagct cgctcattcc aattccttct
      361 attaggctac taacaccatg actttattag cctgtctatc ctggcccccc tggcgaggtt
      421 catgtttgtt tatttccgaa tgcaacaagc tccgcattac acccgaacat cactccagat
      481 gagggctttc tgagtgtggg gtcaaatagt ttcatgttcc ccaaatggcc caaaactgac
      541 agtttaaacg ctgtcttgga acctaatatg acaaaagcgt gatctcatcc aagatgaact
      601 aagtttggtt cgttgaaatg ctaacggcca gttggtcaaa aagaaacttc caaaagtcgg
      661 cataccgttt gtcttgtttg gtattgattg acgaatgctc aaaaataatc tcattaatgc
      721 ttagcgcagt ctctctatcg cttctgaacc ccggtgcacc tgtgccgaaa cgcaaatggg
      781 gaaacacccg ctttttggat gattatgcat tgtctccaca ttgtatgctt ccaagattct
      841 ggtgggaata ctgctgatag cctaacgttc atgatcaaaa tttaactgtt ctaaccccta
      901 cttgacagca atatataaac agaaggaagc tgccctgtct taaacctttt tttttatcat
      961 cattattagc ttactttcat aattgcgact ggttccaatt gacaagcttt tgattttaac
     1021 gacttttaac gacaacttga gaagatcaaa aaacaactaa ttattgaaag aattcactag
     1081 tggcgcgccg cggccgctca agaggatgtc agaatgccat ttgcctgaga gatgcaggct
     1141 tcatttttga tactttttta tttgtaacct atatagtata ggattttttt tgtcattttg
     1201 tttcttctcg tacgagcttg ctcctgatca gcctatctcg cagcagatga atatcttgtg
     1261 gtaggggttt gggaaaatca ttcgagtttg atgtttttct tggtatttcc cactcctctt
     1321 cagagtacag aagattaagt gagaccttcg tttgtgcgga tccttcagta atgtcttgtt
     1381 tcttttgttg cagtggtgag ccattttgac ttcgtgaaag tttctttaga atagttgttt
     1441 ccagaggcca aacattccac ccgtagtaaa gtgcaagcgt aggaagacca agactggcat
     1501 aaatcaggta taagtgtcga gcactggcag gtgatcttct gaaagtttct actagcagat
     1561 aagatccagt agtcatgcat atggcaacaa tgtaccgtgt ggatctaaga acgcgtccta
     1621 ctaaccttcg cattcgttgg tccagtttgt tgttatcgat caacgtgaca aggttgtcga
     1681 ttccgcgtaa gcatgcatac ccaaggacgc ctgttgcaat tccaagtgag ccagttccaa
     1741 caatctttgt aatattagag cacttcattg tgttgcgctt gaaagtaaaa tgcgaacaaa
     1801 ttaagagata atctcgaaac cgcgacttca aacgccaata tgatgtgcgg cacacaataa
     1861 gcgttcatat ccgctgggtg actttctcgc tttaaaaaat tatccgaaaa aattttctag
     1921 agtgttgtta ctttatactt ccggctcgta taatacgaca aggtgtaagg aggactaaac
     1981 catggctaaa ctcacctctg ctgttccagt cctgactgct cgtgatgttg ctggtgctgt
     2041 tgagttctgg actgatagac tcggtttctc ccgtgacttc gtagaggacg actttgccgg
     2101 tgttgtacgt gacgacgtta ccctgttcat ctccgcagtt caggaccagg ttgtgccaga
     2161 caacactctg gcatgggtat gggttcgtgg tctggacgaa ctgtacgctg agtggtctga
     2221 ggtcgtgtct accaacttcc gtgatgcatc tggtccagct atgaccgaga tcggtgaaca
     2281 gccctggggt cgtgagtttg cactgcgtga tccagctggt aactgcgtgc atttcgtcgc
     2341 agaagagcag gactaacaat tgacacctta cgattattta gagagtattt attagtttta
     2401 ttgtatgtat acggatgttt tattatctat ttatgccctt atattctgta actatccaaa
     2461 agtcctatct tatcaagcca gcaatctatg tccgcgaacg tcaactaaaa ataagctttt
     2521 tatgctcttc tctctttttt tcccttcggt ataattatac cttgcatcca cagattctcc
     2581 tgccaaattt tgcataatcc tttacaacat ggctatatgg gagcacttag cgccctccaa
     2641 aacccatatt gcctacgcat gtataggtgt tttttccaca atattttctc tgtgctctct
     2701 ttttattaaa gagaagctct atatcggaga agcttctgtg gccgttatat tcggccttat
     2761 cgtgggacca cattgcctga attggtttgc cccggaagat tggggaaact tggatctgat
     2821 taccttagct gcaggtacca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2881 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     2941 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3001 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3061 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3121 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3181 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3241 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3301 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3361 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3421 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3481 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3541 gtaccc
//
