LOCUS       40924_35424        3985 bp DNA     circular SYN 18-DEC-2018
DEFINITION  E.coli-K.pastoris shuttle vector pPpKan_S, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Naatsaari L, Mistlberger B, Ruth C, Hajek T, Hartner FS, Glieder A.
  TITLE     Deletion of the Pichia pastoris KU70 Homologue Facilitates Platform 
            Strain Generation for Gene Expression and Synthetic Biology
  JOURNAL   PLoS ONE 7 (6), E39720 (2012)
  PUBMED    22768112
REFERENCE   2  (bases 1 to 3985)
  AUTHORS   Naeaetsaari L, Mistlberger B, Ruth C, Hajek T, Hartner F, Glieder A.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2012) Institute of Molecular Biotechnology, Graz 
            University of Technology, Petersgasse 14/2, Graz, Styria 8010, 
            Austria
REFERENCE   3  (bases 1 to 3985)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3985)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2012"; volume: "7"; issue: "6"; pages: "E39720"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-JAN-2012) Institute of Molecular Biotechnology, Graz University 
            of Technology, Petersgasse 14/2, Graz, Styria 8010, Austria"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: SeqMan v. 7.0.0
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3985
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        139..1071
                     /label=AOX1 promoter
                     /note="inducible promoter, regulated by methanol"
     terminator      1100..1346
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     regulatory      1361..1918
                     /label=ILV5 promoter
                     /note="ILV5 promoter"
                     /regulatory_class="promoter"
     regulatory      1919..1982
                     /label=EM72 synthetic promoter
                     /note="EM72 synthetic promoter"
                     /regulatory_class="promoter"
     CDS             1984..2790
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      2802..3272
                     /label=AOD terminator
                     /note="AOD terminator"
                     /regulatory_class="terminator"
     rep_origin      3319..3907
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatccaatt cccgctttga ctgcctgaaa tctccatcgc ctacaatgat gacatttgga
       61 tttggttgac tcatgttggt attgtgaaat agacgcagat cgggaacact gaaaaataca
      121 cagttattat tcatttaaat aacatccaaa gacgaaaggt tgaatgaaac ctttttgcca
      181 tccgacatcc acaggtccat tctcacacat aagtgccaaa cgcaacagga ggggatacac
      241 tagcagcaga ccgttgcaaa cgcaggacct ccactcctct tctcctcaac acccactttt
      301 gccatcgaaa aaccagccca gttattgggc ttgattggag ctcgctcatt ccaattcctt
      361 ctattaggct actaacacca tgactttatt agcctgtcta tcctggcccc cctggcgagg
      421 ttcatgtttg tttatttccg aatgcaacaa gctccgcatt acacccgaac atcactccag
      481 atgagggctt tctgagtgtg gggtcaaata gtttcatgtt ccccaaatgg cccaaaactg
      541 acagtttaaa cgctgtcttg gaacctaata tgacaaaagc gtgatctcat ccaagatgaa
      601 ctaagtttgg ttcgttgaaa tgctaacggc cagttggtca aaaagaaact tccaaaagtc
      661 ggcataccgt ttgtcttgtt tggtattgat tgacgaatgc tcaaaaataa tctcattaat
      721 gcttagcgca gtctctctat cgcttctgaa ccccggtgca cctgtgccga aacgcaaatg
      781 gggaaacacc cgctttttgg atgattatgc attgtctcca cattgtatgc ttccaagatt
      841 ctggtgggaa tactgctgat agcctaacgt tcatgatcaa aatttaactg ttctaacccc
      901 tacttgacag caatatataa acagaaggaa gctgccctgt cttaaacctt tttttttatc
      961 atcattatta gcttactttc ataattgcga ctggttccaa ttgacaagct tttgatttta
     1021 acgactttta acgacaactt gagaagatca aaaaacaact aattattgaa agaattcact
     1081 agtggcgcgc cgcggccgct caagaggatg tcagaatgcc atttgcctga gagatgcagg
     1141 cttcattttt gatacttttt tatttgtaac ctatatagta taggattttt tttgtcattt
     1201 tgtttcttct cgtacgagct tgctcctgat cagcctatct cgcagcagat gaatatcttg
     1261 tggtaggggt ttgggaaaat cattcgagtt tgatgttttt cttggtattt cccactcctc
     1321 ttcagagtac agaagattaa gtgagacctt cgtttgtgcg gatccttcag taatgtcttg
     1381 tttcttttgt tgcagtggtg agccattttg acttcgtgaa agtttcttta gaatagttgt
     1441 ttccagaggc caaacattcc acccgtagta aagtgcaagc gtaggaagac caagactggc
     1501 ataaatcagg tataagtgtc gagcactggc aggtgatctt ctgaaagttt ctactagcag
     1561 ataagatcca gtagtcatgc atatggcaac aatgtaccgt gtggatctaa gaacgcgtcc
     1621 tactaacctt cgcattcgtt ggtccagttt gttgttatcg atcaacgtga caaggttgtc
     1681 gattccgcgt aagcatgcat acccaaggac gcctgttgca attccaagtg agccagttcc
     1741 aacaatcttt gtaatattag agcacttcat tgtgttgcgc ttgaaagtaa aatgcgaaca
     1801 aattaagaga taatctcgaa accgcgactt caaacgccaa tatgatgtgc ggcacacaat
     1861 aagcgttcat atccgctggg tgactttctc gctttaaaaa attatccgaa aaaattttct
     1921 agagtgttga cactttatac ttccggctcg tataatacga caaggtgtaa ggaggactaa
     1981 accatgggta aggaaaagac tcacgtttcg aggccgcgat taaattccaa catggatgct
     2041 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat
     2101 cgattgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
     2161 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
     2221 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc
     2281 cccggcaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
     2341 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
     2401 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt
     2461 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa
     2521 atgcataagc ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt
     2581 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga
     2641 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct
     2701 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg
     2761 cagtttcatt tgatgctcga tgagtttttc taacctctag gacaccttac gattatttag
     2821 agagtattta ttagttttat tgtatgtata cggatgtttt attatctatt tatgccctta
     2881 tattctgtaa ctatccaaaa gtcctatctt atcaagccag caatctatgt ccgcgaacgt
     2941 caactaaaaa taagcttttt atgctcttct ctcttttttt cccttcggta taattatacc
     3001 ttgcatccac agattctcct gccaaatttt gcataatcct ttacaacatg gctatatggg
     3061 agcacttagc gccctccaaa acccatattg cctacgcatg tataggtgtt ttttccacaa
     3121 tattttctct gtgctctctt tttattaaag agaagctcta tatcggagaa gcttctgtgg
     3181 ccgttatatt cggccttatc gtgggaccac attgcctgaa ttggtttgcc ccggaagatt
     3241 ggggaaactt ggatctgatt accttagctg caggtaccac tgagcgtcag accccgtaga
     3301 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     3361 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     3421 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     3481 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     3541 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     3601 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     3661 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     3721 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     3781 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     3841 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     3901 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     3961 tcacatgttc tttcctgcgg taccc
//
