LOCUS       40924_35394        3690 bp DNA     circular SYN 18-DEC-2018
DEFINITION  E.coli-K.pastoris shuttle vector pPpB1_S, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Naatsaari L, Mistlberger B, Ruth C, Hajek T, Hartner FS, Glieder A.
  TITLE     Deletion of the Pichia pastoris KU70 Homologue Facilitates Platform 
            Strain Generation for Gene Expression and Synthetic Biology
  JOURNAL   PLoS ONE 7 (6), E39720 (2012)
  PUBMED    22768112
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   Naeaetsaari L, Mistlberger B, Ruth C, Hajek T, Hartner F, Glieder A.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2012) Institute of Molecular Biotechnology, Graz 
            University of Technology, Petersgasse 14/2, Graz, Styria 8010, 
            Austria
REFERENCE   3  (bases 1 to 3690)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3690)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2012"; volume: "7"; issue: "6"; pages: "E39720"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-JAN-2012) Institute of Molecular Biotechnology, Graz University 
            of Technology, Petersgasse 14/2, Graz, Styria 8010, Austria"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: SeqMan v. 7.0.0
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        139..1071
                     /label=AOX1 promoter
                     /note="inducible promoter, regulated by methanol"
     terminator      1100..1346
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        1509..2213
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     regulatory      2232..2289
                     /label=EM72 synthetic promoter
                     /note="EM72 synthetic promoter"
                     /regulatory_class="promoter"
     CDS             2292..2663
                     /label=BleoR
                     /note="antibiotic-binding protein"
     terminator      2673..2860
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     rep_origin      3024..3612
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatccaatt cccgctttga ctgcctgaaa tctccatcgc ctacaatgat gacatttgga
       61 tttggttgac tcatgttggt attgtgaaat agacgcagat cgggaacact gaaaaataca
      121 cagttattat tcatttaaat aacatccaaa gacgaaaggt tgaatgaaac ctttttgcca
      181 tccgacatcc acaggtccat tctcacacat aagtgccaaa cgcaacagga ggggatacac
      241 tagcagcaga ccgttgcaaa cgcaggacct ccactcctct tctcctcaac acccactttt
      301 gccatcgaaa aaccagccca gttattgggc ttgattggag ctcgctcatt ccaattcctt
      361 ctattaggct actaacacca tgactttatt agcctgtcta tcctggcccc cctggcgagg
      421 ttcatgtttg tttatttccg aatgcaacaa gctccgcatt acacccgaac atcactccag
      481 atgagggctt tctgagtgtg gggtcaaata gtttcatgtt ccccaaatgg cccaaaactg
      541 acagtttaaa cgctgtcttg gaacctaata tgacaaaagc gtgatctcat ccaagatgaa
      601 ctaagtttgg ttcgttgaaa tgctaacggc cagttggtca aaaagaaact tccaaaagtc
      661 ggcataccgt ttgtcttgtt tggtattgat tgacgaatgc tcaaaaataa tctcattaat
      721 gcttagcgca gtctctctat cgcttctgaa ccccggtgca cctgtgccga aacgcaaatg
      781 gggaaacacc cgctttttgg atgattatgc attgtctcca cattgtatgc ttccaagatt
      841 ctggtgggaa tactgctgat agcctaacgt tcatgatcaa aatttaactg ttctaacccc
      901 tacttgacag caatatataa acagaaggaa gctgccctgt cttaaacctt tttttttatc
      961 atcattatta gcttactttc ataattgcga ctggttccaa ttgacaagct tttgatttta
     1021 acgactttta acgacaactt gagaagatca aaaaacaact aattattgaa agaattcact
     1081 agtggcgcgc cgcggccgct caagaggatg tcagaatgcc atttgcctga gagatgcagg
     1141 cttcattttt gatacttttt tatttgtaac ctatatagta taggattttt tttgtcattt
     1201 tgtttcttct cgtacgagct tgctcctgat cagcctatct cgcagcagat gaatatcttg
     1261 tggtaggggt ttgggaaaat cattcgagtt tgatgttttt cttggtattt cccactcctc
     1321 ttcagagtac agaagattaa gtgagacctt cgtttgtgcg gatcctttat tatgttacaa
     1381 tatggaaggg aactttacac ttctcctatg cacatatatt aattaaagtc caatgctagt
     1441 agagaagggg ggtaacaccc ctccgcgctc ttttccgatt tttttctaaa ccgtggaata
     1501 tttcggatat ccttttgttg tttccgggtg tacaatatgg acttcctctt ttctggcaac
     1561 caaacccata catcgggatt cctataatac cttcgttggt ctccctaaca tgtaggtggc
     1621 ggaggggaga tatacaatag aacagatacc agacaagaca taatgggcta aacaagacta
     1681 caccaattac actgcctcat tgatggtggt acataacgaa ctaatactgt agccctagac
     1741 ttgatagcca tcatcatatc gaagtttcac tacccttttt ccatttgcca tctattgaag
     1801 taataatagg cgcatgcaac ttcttttctt tttttttctt ttctctctcc cccgttgttg
     1861 tctcaccata tccgcaatga caaaaaaatg atggaagaca ctaaaggaaa aaattaacga
     1921 caaagacagc accaacagat gtcgttgttc cagagctgat gaggggtatc tcgaagcaca
     1981 cgaaactttt tccttccttc attcacgcac actactctct aatgagcaac ggtatacggc
     2041 cttccttcca gttacttgaa tttgaaataa aaaaaagttt gctgtcttgc tatcaagtat
     2101 aaatagacct gcaattatta atcttttgtt tcctcgtcat tgttctcgtt ccctttcttc
     2161 cttgtttctt tttctgcaca atatttcaag ctataccaag catacaatca actatctcat
     2221 atacatctag agtgttgaca ctttatactt ccggctcgta taatacgaca aggtgtaagg
     2281 aggactaaac catggctaaa ctcacctctg ctgttccagt cctgactgct cgtgatgttg
     2341 ctggtgctgt tgagttctgg actgatagac tcggtttctc ccgtgacttc gtagaggacg
     2401 actttgccgg tgttgtacgt gacgacgtta ccctgttcat ctccgcagtt caggaccagg
     2461 ttgtgccaga caacactctg gcatgggtat gggttcgtgg tctggacgaa ctgtacgctg
     2521 agtggtctga ggtcgtgtct accaacttcc gtgatgcatc tggtccagct atgaccgaga
     2581 tcggtgaaca gccctggggt cgtgagtttg cactgcgtga tccagctggt aactgcgtgc
     2641 atttcgtcgc agaagagcag gactaaccta gggcgaattt cttatgattt atgattttta
     2701 ttattaaata agttataaaa aaaataagtg tatacaaatt ttaaagtgac tcttaggttt
     2761 taaaacgaaa attcttattc ttgagtaact ctttcctgta ggtcaggttg ctttctcagg
     2821 tatagcatga ggtcgctctt attgaccaca cctctaccgg catgccgagc aaatgcctgc
     2881 aaatcgctcc ccatttcacc caattgtaga tatgctaact ccagcaatga gttgatgaat
     2941 ctcggtgtgt attttatgtc ctcagaggac aactgcaggt accactgagc gtcagacccc
     3001 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     3061 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     3121 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     3181 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     3241 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     3301 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     3361 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     3421 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     3481 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     3541 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     3601 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     3661 tttgctcaca tgttctttcc tgcggtaccc
//
