LOCUS       40924_35384        3245 bp DNA     circular SYN 18-DEC-2018
DEFINITION  E.coli-K.pastoris shuttle vector pPpB1GAP_S, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Naatsaari L, Mistlberger B, Ruth C, Hajek T, Hartner FS, Glieder A.
  TITLE     Deletion of the Pichia pastoris KU70 Homologue Facilitates Platform 
            Strain Generation for Gene Expression and Synthetic Biology
  JOURNAL   PLoS ONE 7 (6), E39720 (2012)
  PUBMED    22768112
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   Naeaetsaari L, Mistlberger B, Ruth C, Hajek T, Hartner F, Glieder A.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2012) Institute of Molecular Biotechnology, Graz 
            University of Technology, Petersgasse 14/2, Graz, Styria 8010, 
            Austria
REFERENCE   3  (bases 1 to 3245)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3245)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2012"; volume: "7"; issue: "6"; pages: "E39720"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-JAN-2012) Institute of Molecular Biotechnology, Graz University 
            of Technology, Petersgasse 14/2, Graz, Styria 8010, Austria"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: SeqMan v. 7.0.0
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        141..617
                     /label=GAP promoter
                     /note="promoter for Pichia pastoris
                     glyceraldehyde-3-phosphate dehydrogenase"
     terminator      655..901
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        1064..1768
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     regulatory      1787..1844
                     /label=EM72 synthetic promoter
                     /note="EM72 synthetic promoter"
                     /regulatory_class="promoter"
     CDS             1847..2218
                     /label=BleoR
                     /note="antibiotic-binding protein"
     terminator      2228..2415
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     rep_origin      2579..3167
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatccaatt cccgctttga ctgcctgaaa tctccatcgc ctacaatgat gacatttgga
       61 tttggttgac tcatgttggt attgtgaaat agacgcagat cgggaacact gaaaaataca
      121 cagttattat tcatttaaat tttttgtaga aatgtcttgg tgtcctcgtc caatcaggta
      181 gccatctctg aaatatctgg ctccgttgca actccgaacg acctgctggc aacgtaaaat
      241 tctccggggt aaaacttaaa tgtggagtaa tggaaccaga aacgtctctt cccttctctc
      301 tccttccacc gcccgttacc gtccctagga aattttactc tgctggagag cttcttctac
      361 ggcccccttg cagcaatgct cttcccagca ttacgttgcg ggtaaaacgg aggtcgtgta
      421 cccgacctag cagcccaggg atggaaaagt cccggccgtc gctggcaata atagcgggcg
      481 gacgcatgtc atgagattat tggaaaccac cagaatcgaa tataaaaggc gaacaccttt
      541 cccaattttg gtttctcctg acccaaagac tttaaattta atttatttgt ccctatttca
      601 atcaattgaa caactatcaa aacacagaat tcactagtgg cgcgccgcgg ccgctcaaga
      661 ggatgtcaga atgccatttg cctgagagat gcaggcttca tttttgatac ttttttattt
      721 gtaacctata tagtatagga ttttttttgt cattttgttt cttctcgtac gagcttgctc
      781 ctgatcagcc tatctcgcag cagatgaata tcttgtggta ggggtttggg aaaatcattc
      841 gagtttgatg tttttcttgg tatttcccac tcctcttcag agtacagaag attaagtgag
      901 accttcgttt gtgcggatcc tttattatgt tacaatatgg aagggaactt tacacttctc
      961 ctatgcacat atattaatta aagtccaatg ctagtagaga aggggggtaa cacccctccg
     1021 cgctcttttc cgattttttt ctaaaccgtg gaatatttcg gatatccttt tgttgtttcc
     1081 gggtgtacaa tatggacttc ctcttttctg gcaaccaaac ccatacatcg ggattcctat
     1141 aataccttcg ttggtctccc taacatgtag gtggcggagg ggagatatac aatagaacag
     1201 ataccagaca agacataatg ggctaaacaa gactacacca attacactgc ctcattgatg
     1261 gtggtacata acgaactaat actgtagccc tagacttgat agccatcatc atatcgaagt
     1321 ttcactaccc tttttccatt tgccatctat tgaagtaata ataggcgcat gcaacttctt
     1381 ttcttttttt ttcttttctc tctcccccgt tgttgtctca ccatatccgc aatgacaaaa
     1441 aaatgatgga agacactaaa ggaaaaaatt aacgacaaag acagcaccaa cagatgtcgt
     1501 tgttccagag ctgatgaggg gtatctcgaa gcacacgaaa ctttttcctt ccttcattca
     1561 cgcacactac tctctaatga gcaacggtat acggccttcc ttccagttac ttgaatttga
     1621 aataaaaaaa agtttgctgt cttgctatca agtataaata gacctgcaat tattaatctt
     1681 ttgtttcctc gtcattgttc tcgttccctt tcttccttgt ttctttttct gcacaatatt
     1741 tcaagctata ccaagcatac aatcaactat ctcatataca tctagagtgt tgacacttta
     1801 tacttccggc tcgtataata cgacaaggtg taaggaggac taaaccatgg ctaaactcac
     1861 ctctgctgtt ccagtcctga ctgctcgtga tgttgctggt gctgttgagt tctggactga
     1921 tagactcggt ttctcccgtg acttcgtaga ggacgacttt gccggtgttg tacgtgacga
     1981 cgttaccctg ttcatctccg cagttcagga ccaggttgtg ccagacaaca ctctggcatg
     2041 ggtatgggtt cgtggtctgg acgaactgta cgctgagtgg tctgaggtcg tgtctaccaa
     2101 cttccgtgat gcatctggtc cagctatgac cgagatcggt gaacagccct ggggtcgtga
     2161 gtttgcactg cgtgatccag ctggtaactg cgtgcatttc gtcgcagaag agcaggacta
     2221 acctagggcg aatttcttat gatttatgat ttttattatt aaataagtta taaaaaaaat
     2281 aagtgtatac aaattttaaa gtgactctta ggttttaaaa cgaaaattct tattcttgag
     2341 taactctttc ctgtaggtca ggttgctttc tcaggtatag catgaggtcg ctcttattga
     2401 ccacacctct accggcatgc cgagcaaatg cctgcaaatc gctccccatt tcacccaatt
     2461 gtagatatgc taactccagc aatgagttga tgaatctcgg tgtgtatttt atgtcctcag
     2521 aggacaactg caggtaccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     2581 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     2641 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     2701 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     2761 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     2821 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     2881 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     2941 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     3001 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     3061 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     3121 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     3181 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgg
     3241 taccc
//
