LOCUS       40924_35379        3243 bp DNA     circular SYN 18-DEC-2018
DEFINITION  E.coli-K.pastoris shuttle vector pPpB1GAP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Naatsaari L, Mistlberger B, Ruth C, Hajek T, Hartner FS, Glieder A.
  TITLE     Deletion of the Pichia pastoris KU70 Homologue Facilitates Platform 
            Strain Generation for Gene Expression and Synthetic Biology
  JOURNAL   PLoS ONE 7 (6), E39720 (2012)
  PUBMED    22768112
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Naeaetsaari L, Mistlberger B, Ruth C, Hajek T, Hartner F, Glieder A.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2012) Institute of Molecular Biotechnology, Graz 
            University of Technology, Petersgasse 14/2, Graz, Styria 8010, 
            Austria
REFERENCE   3  (bases 1 to 3243)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3243)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2012"; volume: "7"; issue: "6"; pages: "E39720"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-JAN-2012) Institute of Molecular Biotechnology, Graz University 
            of Technology, Petersgasse 14/2, Graz, Styria 8010, Austria"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: SeqMan v. 7.0.0
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        139..615
                     /label=GAP promoter
                     /note="promoter for Pichia pastoris
                     glyceraldehyde-3-phosphate dehydrogenase"
     terminator      653..899
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        1062..1766
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     regulatory      1785..1842
                     /label=EM72 synthetic promoter
                     /note="EM72 synthetic promoter"
                     /regulatory_class="promoter"
     CDS             1845..2216
                     /label=BleoR
                     /note="antibiotic-binding protein"
     terminator      2226..2413
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     rep_origin      2577..3165
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatccaatt cccgctttga ctgcctgaaa tctccatcgc ctacaatgat gacatttgga
       61 tttggttgac tcatgttggt attgtgaaat agacgcagat cgggaacact gaaaaataca
      121 cagttattat tcagatcttt tttgtagaaa tgtcttggtg tcctcgtcca atcaggtagc
      181 catctctgaa atatctggct ccgttgcaac tccgaacgac ctgctggcaa cgtaaaattc
      241 tccggggtaa aacttaaatg tggagtaatg gaaccagaaa cgtctcttcc cttctctctc
      301 cttccaccgc ccgttaccgt ccctaggaaa ttttactctg ctggagagct tcttctacgg
      361 cccccttgca gcaatgctct tcccagcatt acgttgcggg taaaacggag gtcgtgtacc
      421 cgacctagca gcccagggat ggaaaagtcc cggccgtcgc tggcaataat agcgggcgga
      481 cgcatgtcat gagattattg gaaaccacca gaatcgaata taaaaggcga acacctttcc
      541 caattttggt ttctcctgac ccaaagactt taaatttaat ttatttgtcc ctatttcaat
      601 caattgaaca actatcaaaa cacagaattc actagtggcg cgccgcggcc gctcaagagg
      661 atgtcagaat gccatttgcc tgagagatgc aggcttcatt tttgatactt ttttatttgt
      721 aacctatata gtataggatt ttttttgtca ttttgtttct tctcgtacga gcttgctcct
      781 gatcagccta tctcgcagca gatgaatatc ttgtggtagg ggtttgggaa aatcattcga
      841 gtttgatgtt tttcttggta tttcccactc ctcttcagag tacagaagat taagtgagac
      901 cttcgtttgt gcggatcctt tattatgtta caatatggaa gggaacttta cacttctcct
      961 atgcacatat attaattaaa gtccaatgct agtagagaag gggggtaaca cccctccgcg
     1021 ctcttttccg atttttttct aaaccgtgga atatttcgga tatccttttg ttgtttccgg
     1081 gtgtacaata tggacttcct cttttctggc aaccaaaccc atacatcggg attcctataa
     1141 taccttcgtt ggtctcccta acatgtaggt ggcggagggg agatatacaa tagaacagat
     1201 accagacaag acataatggg ctaaacaaga ctacaccaat tacactgcct cattgatggt
     1261 ggtacataac gaactaatac tgtagcccta gacttgatag ccatcatcat atcgaagttt
     1321 cactaccctt tttccatttg ccatctattg aagtaataat aggcgcatgc aacttctttt
     1381 cttttttttt cttttctctc tcccccgttg ttgtctcacc atatccgcaa tgacaaaaaa
     1441 atgatggaag acactaaagg aaaaaattaa cgacaaagac agcaccaaca gatgtcgttg
     1501 ttccagagct gatgaggggt atctcgaagc acacgaaact ttttccttcc ttcattcacg
     1561 cacactactc tctaatgagc aacggtatac ggccttcctt ccagttactt gaatttgaaa
     1621 taaaaaaaag tttgctgtct tgctatcaag tataaataga cctgcaatta ttaatctttt
     1681 gtttcctcgt cattgttctc gttccctttc ttccttgttt ctttttctgc acaatatttc
     1741 aagctatacc aagcatacaa tcaactatct catatacatc tagagtgttg acactttata
     1801 cttccggctc gtataatacg acaaggtgta aggaggacta aaccatggct aaactcacct
     1861 ctgctgttcc agtcctgact gctcgtgatg ttgctggtgc tgttgagttc tggactgata
     1921 gactcggttt ctcccgtgac ttcgtagagg acgactttgc cggtgttgta cgtgacgacg
     1981 ttaccctgtt catctccgca gttcaggacc aggttgtgcc agacaacact ctggcatggg
     2041 tatgggttcg tggtctggac gaactgtacg ctgagtggtc tgaggtcgtg tctaccaact
     2101 tccgtgatgc atctggtcca gctatgaccg agatcggtga acagccctgg ggtcgtgagt
     2161 ttgcactgcg tgatccagct ggtaactgcg tgcatttcgt cgcagaagag caggactaac
     2221 ctagggcgaa tttcttatga tttatgattt ttattattaa ataagttata aaaaaaataa
     2281 gtgtatacaa attttaaagt gactcttagg ttttaaaacg aaaattctta ttcttgagta
     2341 actctttcct gtaggtcagg ttgctttctc aggtatagca tgaggtcgct cttattgacc
     2401 acacctctac cggcatgccg agcaaatgcc tgcaaatcgc tccccatttc acccaattgt
     2461 agatatgcta actccagcaa tgagttgatg aatctcggtg tgtattttat gtcctcagag
     2521 gacaactgca ggtaccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     2581 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     2641 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     2701 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     2761 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     2821 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     2881 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     2941 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     3001 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     3061 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     3121 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     3181 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcggta
     3241 ccc
//
