LOCUS       V003955                 6420 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003955
VERSION     V003955
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6420)
  AUTHORS   Kolkenbrock S, Moerschbacher BM.
  TITLE     pPolyREPII, a Synthetic, Modular, Broad-Host-Range Expression Vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6420)
  AUTHORS   Kolkenbrock S, Moerschbacher BM.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-SEP-2013) Institute of Plant Biology and
            Biotechnology, WWU Muenster, Schlossplatz 8, Muenster, NRW 48143,
            Germany
REFERENCE   3  (bases 1 to 6420)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6420)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (14-SEP-2013) Institute of Plant Biology and Biotechnology, WWU
            Muenster, Schlossplatz 8, Muenster, NRW 48143, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6420
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        125..153
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    161..177
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      269..296
                     /label="trpA terminator"
                     /note="trpA terminator"
                     /regulatory_class="terminator"
     regulatory      305..332
                     /label="trpA terminator"
                     /note="trpA terminator"
                     /regulatory_class="terminator"
     CDS             1682..1693
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
     regulatory      1891..1929
                     /label="new_Ter terminator"
                     /note="new_Ter terminator"
                     /regulatory_class="terminator"
     CDS             complement(1950..2597)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     rep_origin      3083..3852
                     /label="pBBR1 oriV"
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1
                     Rep protein for replication"
     CDS             3853..4512
                     /label="pBBR1 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     terminator      5128..5175
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     CDS             complement(5179..6258)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(6259..6336)
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
ORIGIN
        1 cctgtgcaag gagtacttcg taaatcactg cataattcgt gtcgctcaag gcgcactccc
       61 gttctggata atgttttttg cgccgacatc ataacggttc tggcaaatat tctgaaatga
      121 gctgttgaca attaatcatc ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt
      181 cacacaggaa acagaattcc atatggatat cgagctcggt acccggggat cctctagagt
      241 cgacctgcag gcatgcaagc ttctcgagaa aaaaaagccc gctcattagg cgggctaaaa
      301 aaaaagcccg cctaatgagc gggctttttt ttacagaaaa gcccgccttt cggcgggctt
      361 tgagcggccg cgtcatttta tttcccccgt ttcagcatca agaacctttg cataacttgc
      421 tctatatcca cactgataat tgccctcaaa ccataatcta aaggcgctag agtttgttga
      481 aacaatatct tttacatcat tcgtatttaa aattccaaac tccgctcccc taaggcgaat
      541 aaaagccatt aaatcttttg tatttaccaa attatagtca tccactatat ctaaaagtaa
      601 attcttcaat tctctttttt ggctttcatc aagtgttata tagcggtcaa tatcaaaatc
      661 attaatgttc aaaatatctt ttttgtcgta tatatgttta ttcttagcaa tagcgtcctt
      721 tgattcatga gtcaaatatt catacgaacc tttgatataa tcaagtatct caacatgagc
      781 aactgaacta ttccccaatt ttcgcttaat cttgttccta acgctttcta ttgttacagg
      841 atttcgtgca atatatataa cgtgatagtg tggtttttta tagtgctttc catttcgtat
      901 aacatcacta ctattccatg tatctttatc ttttttttcg tccatatcgt gtaaaggact
      961 gacagccata gatacgccca aactctctaa tttttctttc caatcattag gaattgagtc
     1021 aggatataat aaaaatccaa aatttctagc tttagtattt ttaatagcca tgatataatt
     1081 accttatcaa aaacaagtag cgaaaactcg tatccttcta aaaacgcgag ctttcgctta
     1141 ttttttttgt tctgattcct ttcttgcata ttcttctata gctaacgccg caaccgcaga
     1201 ttttgaaaaa cctttttgtt tcgccatatc tgttaatttt ttatcttgct cttttgtcag
     1261 agaaatcata actctttttt tcgattctga aatcaccatt taaaaaactc caatcaaata
     1321 attttataaa gttagtgtat cactttgtaa tcataaaaac aacaataaag ctacttaaat
     1381 atagatttat aaaaaacgtt ggcgaaaacg ttggcgattc gttggcgatt gaaaaacccc
     1441 ttaaaccctt gagccagttg ggatagagcg tttttggcac aaaaattggc actcggcact
     1501 taatgggggg tcgtagtacg gaagcaaaat tcgcttcctt tccccccatt tttttccaaa
     1561 ttccaaattt ttttcaaaaa ttttccagcg ctaccgctcg gcaaaatgca agcaattttt
     1621 aaaatcaaac ccatgaggga atttcattcc ctcatactcc cttgagcctc ctccaaccga
     1681 aatagaaggg cgctgcgctt attatttcat tcagtcatcg gctttcataa tctaacagac
     1741 aacatcttcg ctgcaaagcc acgctacgct caagggcttt tacgctacga taacgcctgt
     1801 tttaacgatt atgccgataa ctaaacgaaa taaacgctaa aacgtctcag aaacgatttt
     1861 gagacgtttt aataaaaaat cggcggccgc aaaaaaaaag gcctgcgatt acccgcaggc
     1921 cttttttttg ctcttcgcga ctagttttac aaaagccagt cattaggcct atctgacaat
     1981 tcctgaatag agttcataaa caatcctgca tgataaccat cacaaacaga atgatgtacc
     2041 tgtaaagata gcggtaaata tattgaatta cctttattaa tgaattttcc tgctgtaata
     2101 atgggtagaa ggtaattact attattattg atatttaagt taaacccagt aaatgaagtc
     2161 catggaataa tagaaagaga aaaagcattt tcaggtatag gtgttttggg aaacaatttc
     2221 cccgaaccat tatatttctc tacatcagaa aggtataaat cataaaactc tttgaagtca
     2281 ttctttacag gagtccaaat accagagaat gttttagata caccatcaaa aattgtataa
     2341 agtggctcta acttatccca ataacctaac tctccgtcgc tattgtaacc agttctaaaa
     2401 gctgtatttg agtttatcac ccttgtcact aagaaaataa atgcagggta aaatttatat
     2461 ccttcttgtt ttatgtttcg gtataaaaca ctaatatcaa tttctgtggt tatactaaaa
     2521 gtcgtttgtt ggttcaaata atgattaaat atctcttttc tcttccaatt gtctaaatca
     2581 attttattaa agttcatttg atatgcctcc taaattttta tctaaagtga atttaggagg
     2641 cttacttgtc tgctttcttc attagaatca atcccacgtg agatcttttt ttaaaagtca
     2701 atattactgt aacataaata tatattttaa aaatatccca ctttatccaa ttttcgtttg
     2761 ttgaactaat gggtgcttta gttgaagatg cttgagactg gccgccacgt tgcccatttt
     2821 cgccagcttc ttgcatcgca tgatcgcgta tgccgccatg cctgcccctc ccttttggtg
     2881 tccaaccggc tcgacggggg cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg
     2941 agtatactca ctagactttg cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc
     3001 tacgggcttg ctctccgggc ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg
     3061 gatatgtgga cgatggccgc gagcggccac cggctggctc gcttcgctcg gcccgtggac
     3121 aaccctgctg gacaagctga tggacaggct gcgcctgccc acgagcttga ccacagggat
     3181 tgcccaccgg ctacccagcc ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg
     3241 gccttgcccc atcaattttt ttaattttct ctggggaaaa gcctccggcc tgcggcctgc
     3301 gcgcttcgct tgccggttgg acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg
     3361 cgcagcggct tggccttgac gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc
     3421 gaaggcgaag cccgcccgcc tgccccccga gcctcacggc ggcgagtgcg ggggttccaa
     3481 gggggcagcg ccaccttggg caaggccgaa ggccgcgcag tcgatcaaca agccccggag
     3541 gggccacttt ttgccggagg gggagccgcg ccgaaggcgt gggggaaccc cgcaggggtg
     3601 cccttctttg ggcaccaaag aactagatat agggcgaaat gcgaaagact taaaaatcaa
     3661 caacttaaaa aaggggggta cgcaacagct cattgcggca ccccccgcaa tagctcattg
     3721 cgtaggttaa agaaaatctg taattgactg ccacttttac gcaacgcata attgttgtcg
     3781 cgctgccgaa aagttgcagc tgattgcgca tggtgccgca accgtgcggc accctaccgc
     3841 atggagataa gcatggccac gcagtccaga gaaatcggca ttcaagccaa gaacaagccc
     3901 ggtcactggg tgcaaacgga acgcaaagcg catgaggcgt gggccgggct tattgcgagg
     3961 aaacccacgg cggcaatgct gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc
     4021 gtggtggtca gccagaagac actttccaag ctcatcggac gttctttgcg gacggtccaa
     4081 tacgcagtca aggacttggt ggccgagcgc tggatctccg tcgtgaagct caacggcccc
     4141 ggcaccgtgt cggcctacgt ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag
     4201 ttgcgcctgt cggtgttcag tgccgccgtg gtggttgatc acgacgacca ggacgaatcg
     4261 ctgttggggc atggcgacct gcgccgcatc ccgaccctgt atccgggcga gcagcaacta
     4321 ccgaccggcc ccggcgagga gccgcccagc cagcccggca ttccgggcat ggaaccagac
     4381 ctgccagcct tgaccgaaac ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg
     4441 cccgatgagc cgtgttttct ggacgatggc gagccgttgg agccgccgac acgggtcacg
     4501 ctgccgcgcc ggtagcactt gggttgcgca gcaacccgta agtgcgctgt tccagactat
     4561 cggctgtagc cgcctcgccg ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc
     4621 atggagccgg gccacctcga cctgaatgga agccggcggc acctcgctaa cggattcacc
     4681 gtttttatca ggctctggga ggcagaataa atgatcatat cgtcaattat tacctccacg
     4741 gggagagcct gagcaaactg gcctcaggca tttgagaagc acacggtcac actgcttccg
     4801 gtagtcaata aaccggtaaa ccagcaatag acataagcgg ctatttaacg accctgccct
     4861 gaaccgacga ccgggtcgaa tttgctttcg aatttctgcc attcatccgc ttattatcac
     4921 ttattcaggc gtagcaccag gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc
     4981 cccgccctgc cactcatcgc agtcggccta ttggttaaaa aatgagctga tttaacaaaa
     5041 atttaacgcg aattttaaca aaatattaac gcttacaatt tccattcgcc attcaggctg
     5101 cgcaactgtt gggaagggcg atagatctta gcataacccc ttggggcctc taaacgggtc
     5161 ttgaggggtt ttttgccact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     5221 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt
     5281 tcttttcacc agtgagacgg gcaacagctg attgcccttc accgcctggc cctgagagag
     5341 ttgcagcaag cggtccacgc tggtttgccc cagcaggcga aaatcctgtt tgatggtggt
     5401 tgacggcggg atataacatg agctgtcttc ggtatcgtcg tatcccacta ccgagatgtc
     5461 cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc
     5521 gttggcaacc agcatcgcag tgggaacgat gccctcattc agcatttgca tggtttgttg
     5581 aaaaccggac atggcactcc agtcgccttc ccgttccgct atcggctgaa tttgattgcg
     5641 agtgagatat ttatgccagc cagccagacg cagacgcgcc gagacagaac ttaatgggcc
     5701 cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt
     5761 accgtcttca tgggagaaaa taatactgtt gatgggtgtc tggtcagaga catcaagaaa
     5821 taacgccgga acattagtgc aggcagcttc cacagcaatg gcatcctggt catccagcgg
     5881 atagttaatg atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca
     5941 ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg ctggcaccca gttgatcggc
     6001 gcgagattta atcgccgcga caatttgcga cggcgcgtgc agggccagac tggaggtggc
     6061 aacgccaatc agcaacgact gtttgcccgc cagttgttgt gccacgcggt tgggaatgta
     6121 attcagctcc gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc
     6181 ctggttcacc acgcgggaaa cggtctgata agagacaccg gcatactctg cgacatcgta
     6241 taacgttact ggtttcacat tcaccaccct gaattgactc tcttccgggc gctatcatgc
     6301 cataccgcga aaggttttgc accattcgat ggtgtcaacg taaatgccgc ttcgccttcg
     6361 gcgcgcgaat tgcaagctga tccggcggag cttatcgact gcacggtggc tgtggtatgg
//
