LOCUS       V003956                 7403 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003956
VERSION     V003956
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7403)
  AUTHORS   Kolkenbrock S, Moerschbacher BM.
  TITLE     pPolyREPII, a Synthetic, Modular, Broad-Host-Range Expression Vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7403)
  AUTHORS   Kolkenbrock S, Moerschbacher BM.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-SEP-2013) Institute of Plant Biology and
            Biotechnology, WWU Muenster, Schlossplatz 8, Muenster, NRW 48143,
            Germany
REFERENCE   3  (bases 1 to 7403)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7403)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (14-SEP-2013) Institute of Plant Biology and Biotechnology, WWU
            Muenster, Schlossplatz 8, Muenster, NRW 48143, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7403
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        125..153
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    161..177
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      269..296
                     /label="trpA terminator"
                     /note="trpA terminator"
                     /regulatory_class="terminator"
     regulatory      305..332
                     /label="trpA terminator"
                     /note="trpA terminator"
                     /regulatory_class="terminator"
     regulatory      333..362
                     /label="t500 terminator"
                     /note="t500 terminator"
                     /regulatory_class="terminator"
     CDS             complement(1083..1094)
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
     regulatory      2559..2593
                     /label="tR2 terminator"
                     /note="tR2 terminator"
                     /regulatory_class="terminator"
     regulatory      2603..2636
                     /label="tR2 terminator"
                     /note="tR2 terminator"
                     /regulatory_class="terminator"
     CDS             complement(2657..3304)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     rep_origin      3790..4559
                     /label="pBBR1 oriV"
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1
                     Rep protein for replication"
     CDS             4560..5219
                     /label="pBBR1 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     terminator      5838..5919
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      6011..6038
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     CDS             complement(6047..7126)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(7127..7204)
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     regulatory      7292..7322
                     /label="hisT terminator"
                     /note="hisT terminator"
                     /regulatory_class="terminator"
     regulatory      7349..7389
                     /label="hisT terminator"
                     /note="hisT terminator"
                     /regulatory_class="terminator"
ORIGIN
        1 cctgtgcaag gagtacttcg taaatcactg cataattcgt gtcgctcaag gcgcactccc
       61 gttctggata atgttttttg cgccgacatc ataacggttc tggcaaatat tctgaaatga
      121 gctgttgaca attaatcatc ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt
      181 cacacaggaa acagaattcc atatggatat cgagctcggt acccggggat cctctagagt
      241 cgacctgcag gcatgcaagc ttctcgagaa aaaaaagccc gctcattagg cgggctaaaa
      301 aaaaagcccg cctaatgagc gggctttttt ttacagaaaa gcccgccttt cggcgggctt
      361 tgagcggccg caataaaagc cccctgacga aagtcgaagg gggtttttat tttggtttga
      421 tgttgcgatt aatagcaata caattgcaat aaacaaaatg atcgatgctg tttggcaaaa
      481 aaagaaaaag tgattaattt atattttatt tatggcgcta atttattacg gctttttttg
      541 ttgtcggcta gccgattctg atacattttt ttaagcacaa aaaccaccca attttggagt
      601 ggtgtgtaag tgcgcattgt catgaaaaaa tggcacgcaa tttcatcact ttttaaagtg
      661 atgtgtaagt gcgcattgtc atgaaaaaat ggcacgcaat ttcatcactt tttaaagtga
      721 tgtgtaagtg cgcattgttc gaaaaatcga actatgattt atttttgctg ttgtatttat
      781 ttttcatctt ttgggttttg gttttgtttt ttgttgctat cgtagtttat ttgcttttta
      841 agggctctat ttttcgttct acggcatttt tataatttgc caatataatt tatcgatttt
      901 ttattaaaac gtctcaaaat cgtttctgag acgttttagc gtttatttcg tttagttatc
      961 ggcataatcg ttaaaacagg cgttatcgta gcgtaaaagc ccttgagcgt agcgtggctt
     1021 tgcagcgaag atgttgtctg ttagattatg aaagccgatg actgaatgaa ataataagcg
     1081 cagcgccctt ctatttcggt tggaggaggc tcaagggagt atgagggaat gaaattccct
     1141 catgggtttg attttaaaaa ttgcttgcaa ttttgccgag cggtagcgct ggaaaatttt
     1201 tgaaaaaaat ttggaatttg gaaaaaaatg gggggaaagg aagcgaattt tgcttccgta
     1261 ctacgacccc ccattaagtg ccgagtgcca atttttgtgc caaaaacgct ctatcccaac
     1321 tggctcaagg gtttaagggg tttttcaatc gccaacgaat cgccaacgtt ttcgccaacg
     1381 ttttttataa atctatattt aagtagcttt attgttgttt ttatgattac aaagtgatac
     1441 actaacttta taaaattatt tgattggagt tttttaaatg gtgatttcag aatcgaaaaa
     1501 aagagttatg atttctctga caaaagagca agataaaaaa ttaacagata tggcgaaaca
     1561 aaaaggtttt tcaaaatctg cggttgcggc gttagctata gaagaatatg caagaaagga
     1621 atcagaacaa aaaaaataag cgaaagctcg cgtttttaga aggatacgag ttttcgctac
     1681 ttgtttttga taaggtaatt atatcatggc tattaaaaat actaaagcta gaaattttgg
     1741 atttttatta tatcctgact caattcctaa tgattggaaa gaaaaattag agagtttggg
     1801 cgtatctatg gctgtcagtc ctttacacga tatggacgaa aaaaaagata aagatacatg
     1861 gaatagtagt gatgttatac gaaatggaaa gcactataaa aaaccacact atcacgttat
     1921 atatattgca cgaaatcctg taacaataga aagcgttagg aacaagatta agcgaaaatt
     1981 ggggaatagt tcagttgctc atgttgagat acttgattat atcaaaggtt cgtatgaata
     2041 tttgactcat gaatcaaagg acgctattgc taagaataaa catatatacg acaaaaaaga
     2101 tattttgaac attaatgatt ttgatattga ccgctatata acacttgatg aaagccaaaa
     2161 aagagaattg aagaatttac ttttagatat agtggatgac tataatttgg taaatacaaa
     2221 agatttaatg gcttttattc gccttagggg agcggagttt ggaattttaa atacgaatga
     2281 tgtaaaagat attgtttcaa caaactctag cgcctttaga ttatggtttg agggcaatta
     2341 tcagtgtgga tatagagcaa gttatgcaaa ggttcttgat gctgaaacgg gggaaataaa
     2401 atgacaaaca aagaaaaaga gttatttgct gaaaatgagg aattaaaaaa agaaattaag
     2461 gacttaaaag agcgtattga aagatacaga gaaatggaag ttgaattaag tacaacaata
     2521 gatttattga gaggagggat tattgaataa gcggccgcaa aaaaaaaggc ctgcgattac
     2581 cagcaggcct gttttttttt ttaacaggcc tgctggtaat cgcaggcctt ttttttgctc
     2641 ttcgcgacta gttttacaaa agccagtcat taggcctatc tgacaattcc tgaatagagt
     2701 tcataaacaa tcctgcatga taaccatcac aaacagaatg atgtacctgt aaagatagcg
     2761 gtaaatatat tgaattacct ttattaatga attttcctgc tgtaataatg ggtagaaggt
     2821 aattactatt attattgata tttaagttaa acccagtaaa tgaagtccat ggaataatag
     2881 aaagagaaaa agcattttca ggtataggtg ttttgggaaa caatttcccc gaaccattat
     2941 atttctctac atcagaaagg tataaatcat aaaactcttt gaagtcattc tttacaggag
     3001 tccaaatacc agagaatgtt ttagatacac catcaaaaat tgtataaagt ggctctaact
     3061 tatcccaata acctaactct ccgtcgctat tgtaaccagt tctaaaagct gtatttgagt
     3121 ttatcaccct tgtcactaag aaaataaatg cagggtaaaa tttatatcct tcttgtttta
     3181 tgtttcggta taaaacacta atatcaattt ctgtggttat actaaaagtc gtttgttggt
     3241 tcaaataatg attaaatatc tcttttctct tccaattgtc taaatcaatt ttattaaagt
     3301 tcatttgata tgcctcctaa atttttatct aaagtgaatt taggaggctt acttgtctgc
     3361 tttcttcatt agaatcaatc ccacgtgaga tcttttttta aaagtcaata ttactgtaac
     3421 ataaatatat attttaaaaa tatcccactt tatccaattt tcgtttgttg aactaatggg
     3481 tgctttagtt gaagatgctt gagactggcc gccacgttgc ccattttcgc cagcttcttg
     3541 catcgcatga tcgcgtatgc cgccatgcct gcccctccct tttggtgtcc aaccggctcg
     3601 acgggggcag cgcaaggcgg tgcctccggc gggccactca atgcttgagt atactcacta
     3661 gactttgctt cgcaaagtcg tgaccgccta cggcggctgc ggcgccctac gggcttgctc
     3721 tccgggcttc gccctgcgcg gtcgctgcgc tcccttgcca gcccgtggat atgtggacga
     3781 tggccgcgag cggccaccgg ctggctcgct tcgctcggcc cgtggacaac cctgctggac
     3841 aagctgatgg acaggctgcg cctgcccacg agcttgacca cagggattgc ccaccggcta
     3901 cccagccttc gaccacatac ccaccggctc caactgcgcg gcctgcggcc ttgccccatc
     3961 aattttttta attttctctg gggaaaagcc tccggcctgc ggcctgcgcg cttcgcttgc
     4021 cggttggaca ccaagtggaa ggcgggtcaa ggctcgcgca gcgaccgcgc agcggcttgg
     4081 ccttgacgcg cctggaacga cccaagccta tgcgagtggg ggcagtcgaa ggcgaagccc
     4141 gcccgcctgc cccccgagcc tcacggcggc gagtgcgggg gttccaaggg ggcagcgcca
     4201 ccttgggcaa ggccgaaggc cgcgcagtcg atcaacaagc cccggagggg ccactttttg
     4261 ccggaggggg agccgcgccg aaggcgtggg ggaaccccgc aggggtgccc ttctttgggc
     4321 accaaagaac tagatatagg gcgaaatgcg aaagacttaa aaatcaacaa cttaaaaaag
     4381 gggggtacgc aacagctcat tgcggcaccc cccgcaatag ctcattgcgt aggttaaaga
     4441 aaatctgtaa ttgactgcca cttttacgca acgcataatt gttgtcgcgc tgccgaaaag
     4501 ttgcagctga ttgcgcatgg tgccgcaacc gtgcggcacc ctaccgcatg gagataagca
     4561 tggccacgca gtccagagaa atcggcattc aagccaagaa caagcccggt cactgggtgc
     4621 aaacggaacg caaagcgcat gaggcgtggg ccgggcttat tgcgaggaaa cccacggcgg
     4681 caatgctgct gcatcacctc gtggcgcaga tgggccacca gaacgccgtg gtggtcagcc
     4741 agaagacact ttccaagctc atcggacgtt ctttgcggac ggtccaatac gcagtcaagg
     4801 acttggtggc cgagcgctgg atctccgtcg tgaagctcaa cggccccggc accgtgtcgg
     4861 cctacgtggt caatgaccgc gtggcgtggg gccagccccg cgaccagttg cgcctgtcgg
     4921 tgttcagtgc cgccgtggtg gttgatcacg acgaccagga cgaatcgctg ttggggcatg
     4981 gcgacctgcg ccgcatcccg accctgtatc cgggcgagca gcaactaccg accggccccg
     5041 gcgaggagcc gcccagccag cccggcattc cgggcatgga accagacctg ccagccttga
     5101 ccgaaacgga ggaatgggaa cggcgcgggc agcagcgcct gccgatgccc gatgagccgt
     5161 gttttctgga cgatggcgag ccgttggagc cgccgacacg ggtcacgctg ccgcgccggt
     5221 agcacttggg ttgcgcagca acccgtaagt gcgctgttcc agactatcgg ctgtagccgc
     5281 ctcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc
     5341 acctcgacct gaatggaagc cggcggcacc tcgctaacgg attcaccgtt tttatcaggc
     5401 tctgggaggc agaataaatg atcatatcgt caattattac ctccacgggg agagcctgag
     5461 caaactggcc tcaggcattt gagaagcaca cggtcacact gcttccggta gtcaataaac
     5521 cggtaaacca gcaatagaca taagcggcta tttaacgacc ctgccctgaa ccgacgaccg
     5581 ggtcgaattt gctttcgaat ttctgccatt catccgctta ttatcactta ttcaggcgta
     5641 gcaccaggcg tttaagggca ccaataactg ccttaaaaaa attacgcccc gccctgccac
     5701 tcatcgcagt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     5761 tttaacaaaa tattaacgct tacaatttcc attcgccatt caggctgcgc aactgttggg
     5821 aagggcgata gatctataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     5881 tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac
     5941 gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat
     6001 caaattaagc agaaggccat cctgacggat ggcctttttt ttttcactgc ccgctttcca
     6061 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     6121 tttgcgtatt gggcgccagg gtggtttttc ttttcaccag tgagacgggc aacagctgat
     6181 tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg gtttgcccca
     6241 gcaggcgaaa atcctgtttg atggtggttg acggcgggat ataacatgag ctgtcttcgg
     6301 tatcgtcgta tcccactacc gagatgtccg caccaacgcg cagcccggac tcggtaatgg
     6361 cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag catcgcagtg ggaacgatgc
     6421 cctcattcag catttgcatg gtttgttgaa aaccggacat ggcactccag tcgccttccc
     6481 gttccgctat cggctgaatt tgattgcgag tgagatattt atgccagcca gccagacgca
     6541 gacgcgccga gacagaactt aatgggcccg ctaacagcgc gatttgctgg tgacccaatg
     6601 cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata atactgttga
     6661 tgggtgtctg gtcagagaca tcaagaaata acgccggaac attagtgcag gcagcttcca
     6721 cagcaatggc atcctggtca tccagcggat agttaatgat cagcccactg acgcgttgcg
     6781 cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct accatcgaca
     6841 ccaccacgct ggcacccagt tgatcggcgc gagatttaat cgccgcgaca atttgcgacg
     6901 gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag caacgactgt ttgcccgcca
     6961 gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc catcgccgct tccacttttt
     7021 cccgcgtttt cgcagaaacg tggctggcct ggttcaccac gcgggaaacg gtctgataag
     7081 agacaccggc atactctgcg acatcgtata acgttactgg tttcacattc accaccctga
     7141 attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcac cattcgatgg
     7201 tgtcaacgta aatgccgctt cgccttcgcg cgcgaattgc aagctgatcc ggcggagctt
     7261 atcgactgca cggtgcacca agaaagcccc cggaagatgc atcttccggg ggcttttttt
     7321 tttgcttctg gcgtcaggca gccatcggga aagcccccgg aagatgcatc ttccgggggc
     7381 ttttttttta agctgtggta tgg
//
