LOCUS       40924_35324        4975 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPolh-1629, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4975)
  AUTHORS   Liu X.
  TITLE     A Highly Efficient and Simple Construction Strategy for Producing 
            Recombinant Baculovirus Bombyx mori Nucleopolyhedrovirus
  JOURNAL   PLoS ONE 11 (3), E0152140 (2016)
  PUBMED    27008267
REFERENCE   2  (bases 1 to 4975)
  AUTHORS   Liu X.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2016) Biotechnology Research Institute, Chinese 
            Academy of Agricultural Sciences, No. 12 Zhongguancun South Street, 
            Beijing, Beijing 100081, China
REFERENCE   3  (bases 1 to 4975)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4975)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2016"; volume: "11"; issue: "3"; pages: "E0152140"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-FEB-2016) Biotechnology Research Institute, Chinese Academy of 
            Agricultural Sciences, No. 12 Zhongguancun South Street, Beijing, 
            Beijing 100081, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4975
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    434..864
                     /label=homologous arm for BmNPV polyhedrin gene
                     /note="homologous arm for BmNPV polyhedrin gene"
     promoter        878..906
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             954..2141
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     misc_feature    2232..2693
                     /label=homologous arm for BmNPV orf1629 gene
                     /note="homologous arm for BmNPV orf1629 gene"
     primer_bind     complement(2754..2770)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2778..2794)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2802..2832)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2847..2868)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3156..3744)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3918..4775)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4776..4880)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa agaagcatga cggcaagtgg
      421 acgattcgaa ttcctgcgat tgtacatgct gttaacggct ccgcccacta ttaatgcaat
      481 taaaaattcc aattttaaaa aacgcagcaa aagaaacatt tgtatgaaag aatgcgtaga
      541 aggaaaaaat aatgtcgtcg acatgctgaa cagcaagatc aatatgcctc cgtgcataca
      601 aaaaatattg ggcgatttga aaaaaaacaa tgtaccgcgc ggcggtatgt acaggaagag
      661 gtttatacta aactgttaca ttgcaaacgt ggtttcgtgt accaaatgtg aaaaccgatg
      721 tttgatcaag gctctgacac atttttacaa ttacgactcc aagtgtgtgg gtgaagtcat
      781 gcatctttta atcaaatccc aagatgtgta taaaccacca aactgccaaa aaatgaaaac
      841 tgtcgacaag ctctgtccgt ttgcctcgag ctcatgtttg acagcttatc atcgataagc
      901 tttaatgcgg tagtttatca cagttaaatt gctaacgcag tcaggcaccg tgtatgaaat
      961 ctaacaatgc gctcatcgtc atcctcggca ccgtcaccct ggatgctgta ggcataggct
     1021 tggttatgcc ggtactgccg ggcctcttgc gggatatcgt ccattccgac agcatcgcca
     1081 gtcactatgg cgtgctgcta gcgctatatg cgttgatgca atttctatgc gcacccgttc
     1141 tcggagcact gtccgaccgc tttggccgcc gcccagtcct gctcgcttcg ctacttggag
     1201 ccactatcga ctacgcgatc atggcgacca cacccgtcct gtggatcctc tacgccggac
     1261 gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca
     1321 tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg
     1381 gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat
     1441 tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg
     1501 agtcgcataa gggagagcgt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct
     1561 tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc
     1621 aactcgtagg acaggtgccg gcagcgctct gggtcatttt cggcgaggac cgctttcgct
     1681 ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc
     1741 aagccttcgt cactggtccc gccaccaaac gtttcggcga gaagcaggcc attatcgccg
     1801 gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg
     1861 ccttccccat tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca
     1921 tgctgtccag gcaggtagat gacgaccatc agggacagct tcaaggatcg ctcgcggctc
     1981 ttaccagcct aacttcgatc actggaccgc tgatcgtcac ggcgatttat gccgcctcgg
     2041 cgagcacatg gaacgggttg gcatggattg taggcgccgc cctatacctt gtctgcctcc
     2101 ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac ctgaatggaa gccggcggca
     2161 cctcgctaac ggattcacca ctccaagaat tggagccaat caattcttgc ggagaactgt
     2221 gaatgctcga gcaccacacg acttgccaaa tcttgtagca gcaatctagc tttgtcgata
     2281 ttcgtttgtg ttttgttttg taataaagat tcgacgtcgt tcaaaatatt atgcgctttt
     2341 gtattttttt catcactgtc gttggtgtac aattgactcg acgtaaacac gttaaataaa
     2401 gcttggacat atttaacatc gggcgcgtta ggccgattat tgccgccgtc gtcccaaccc
     2461 tcgtcgttag aagttgcttc cgaagacgat tttgccatag ccacacgacg cctattaatt
     2521 gtgtcgacta acacgtccgc gatcaaattt ttagttgttg agtttttcgg aattatttct
     2581 gattgcggac gtttttgtgc gggtttcaat ctaactgtgc ccgattttaa ttcagacaac
     2641 acgttagaaa gcgatggtgc aggcggtggt aacatttcag ccggcaaatc tacctgcaga
     2701 ggatccgaat ctccagagga tcgccgggaa ccgaggacga gttcgtaatc atggtcatag
     2761 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     2821 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     2881 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     2941 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     3001 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     3061 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     3121 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     3181 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     3241 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     3301 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     3361 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     3421 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     3481 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     3541 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     3601 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     3661 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     3721 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     3781 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     3841 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     3901 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     3961 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     4021 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     4081 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     4141 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     4201 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     4261 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     4321 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     4381 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     4441 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     4501 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     4561 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     4621 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     4681 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     4741 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     4801 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     4861 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     4921 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtc
//
