LOCUS       V015589                 4591 bp    DNA     circular SYN 28-OCT-2020
DEFINITION  Exported.
ACCESSION   V015589
VERSION     V015589
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Declercq M, Biquand E, Karim M, Pietrosemoli N, Jacob Y, Demeret C,
            Barbezange C, van der Werf S.
  TITLE     Influenza A virus co-opts ERI1 exonuclease bound to histone mRNA to
            promote viral transcription
  JOURNAL   Nucleic Acids Res 48 (18), 10428-10440 (2020)
   PUBMED   32960265
REFERENCE   2  (bases 1 to 4591)
  AUTHORS   Declercq M, Biquand E, Demeret C, Karim M, Pietrosemoli N, Jacob Y,
            Demeret C, Van der Werf S, Barbezange C.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2020) National Influenza Centre, Sciensano, Rue
            Juliette Wytsman 14, Brussels 1180, Belgium
REFERENCE   3  (bases 1 to 4591)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
            Acids Res"; date: "2020"; volume: "48"; issue: "18"; pages:
            "10428-10440"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (03-SEP-2020) National Influenza Centre, Sciensano, Rue Juliette
            Wytsman 14, Brussels 1180, Belgium"
FEATURES             Location/Qualifiers
     source          1..4591
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        142..172
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     204..220
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      complement(262..345)
                     /label="hepatitis delta ribozyme"
                     /note="hepatitis delta ribozyme"
                     /regulatory_class="terminator"
     misc_feature    complement(346..390)
                     /label="3'NRC"
                     /note="3'NRC"
     CDS             391..1884
                     /gene="NP"
                     /label="Nucleoprotein"
                     /note="Nucleoprotein from Influenza A virus (strain
                     A/Wilson-Smith/1933 H1N1). Accession#: P15682"
     misc_feature    complement(1888..1910)
                     /label="5'NRC"
                     /note="5'NRC"
     regulatory      complement(1911..2048)
                     /label="human PolI promoter"
                     /note="human PolI promoter"
                     /regulatory_class="promoter"
     primer_bind     complement(2195..2211)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        2685..2789
                     /label="AmpR promoter"
     CDS             2790..3647
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3821..4409
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga attcgagctc
      241 ggtacccggg gatcctctag agtcccattc gccattaccg aggggacggt cccctcggaa
      301 tgttgcccag ccggcgccag cgaggaggct gggaccatgc cggccagcaa aagcagggta
      361 gataatcact cacagagtga catcgaaatc atggcgacca aaggcaccaa acgatcttac
      421 gaacagatgg agactgatgg agaacgccag aatgccactg aaatcagagc atctgtcgga
      481 aaaatgattg atggaattgg acgattctac atccaaatgt gcaccgaact taaactcagt
      541 gattatgagg gacggctgat tcagaacagc ttaacaatag agagaatggt gctctctgct
      601 tttgacgaga ggaggaataa atatctagaa gaacatccca gtgcggggaa agatcctaag
      661 aaaactggag gacctatata caggagagta gatggaaagt ggaggagaga actcatcctt
      721 tatgacaaag aagaaataag acgaatctgg cgccaagcta ataatggtga cgatgcaacg
      781 gctggtctga ctcacatgat gatctggcac tccaatttga atgatgcaac ttaccagagg
      841 acaagagctc ttgttcgcac aggaatggat cccaggatgt gctcactgat gcagggttca
      901 accctcccta ggaggtctgg ggccgcaggt gctgcagtca aaggagttgg aacaatggtg
      961 atggaattga tcagaatgat caaacgtggg atcaatgatc ggaacttctg gaggggtgag
     1021 aatggacgga gaacaaggat tgcttatgaa agaatgtgca acattctcaa agggaaattt
     1081 caaacagctg cacaaagaac aatggtggat caagtgagag agagccggaa tccaggaaat
     1141 gctgagttcg aagatctcat ctttttagca cggtctgcac tcatattgag agggtcagtt
     1201 gctcacaagt cctgcctgcc tgcctgtgtg tatggatctg ccgtagccag tggatacgac
     1261 tttgaaagag agggatactc tctagtcgga atagaccctt tcagactgct tcaaaacagc
     1321 caagtataca gcctaatcag accaaatgag aatccagcac acaagagtca actggtgtgg
     1381 atggcatgcc attctgctgc atttgaagat ctaagagtat caagcttcat cagagggacg
     1441 aaagtggtcc caagagggaa gctttccact agaggagttc aaattgcttc caatgaaaac
     1501 atggagacta tggaatcaag tacccttgaa ctgagaagca gatactgggc cataaggacc
     1561 agaagtggag ggaacaccaa tcaacagagg gcttcctcgg gccaaatcag catacaacct
     1621 acgttctcag tacagagaaa tctccctttt gacagaccaa ccattatggc agcattcact
     1681 gggaatacag aggggagaac atctgacatg agaaccgaaa tcataaggct gatggaaagt
     1741 gcaagaccag aagatgtgtc tttccagggg cggggagtct tcgagctctc ggacgaaaag
     1801 gcaacgagcc cgatcgtgcc ctcctttgac atgagtaatg aaggatctta tttcttcgga
     1861 gacaatgcag aggagtacga caattaaaga aaaataccct tgtttctact aataacccgg
     1921 cggcccaaaa tgccgactcg gagcgaaaga tatacctccc ccggggccgg gaggtcgcgt
     1981 caccgaccac gccgccggcc caggcgacgc gcgacacgga cacctgtccc caaaaacgcc
     2041 accatcgcag ccacacacgg agcgcccggg gccctctggt caaccccagg acacacgcgg
     2101 gagcagcgcc gggccgggga cgccctcccg gccgcccgtg ccacacgcag gggccggccc
     2161 gtctagaatt gacctgcagg catgcaagct tggcactggc cgtcgtttta caacgtcgtg
     2221 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     2281 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     2341 atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     2401 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     2461 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     2521 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     2581 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     2641 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     2701 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     2761 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     2821 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     2881 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     2941 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     3001 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     3061 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     3121 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     3181 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     3241 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     3301 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     3361 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     3421 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     3481 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     3541 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     3601 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     3661 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     3721 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     3781 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     3841 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     3901 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     3961 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     4021 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     4081 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     4141 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     4201 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     4261 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     4321 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     4381 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     4441 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     4501 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     4561 gcagcgagtc agtgagcgag gaagcggaag a
//
