LOCUS       40924_34924        8742 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPlat-Pds-Mod4.1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8742)
  AUTHORS   Steinbrenner J, Sandmann G.
  TITLE     Transformation of the green alga Haematococcus pluvialis with a 
            phytoene desaturase for accelerated astaxanthin biosynthesis
  JOURNAL   Appl. Environ. Microbiol. 72 (12), 7477-7484 (2006)
  PUBMED    17012596
REFERENCE   2  (bases 1 to 8742)
  AUTHORS   Steinbrenner J, Sandmann G.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2006) Plant Physiology and Biochemistry, 
            University of Konstanz, Universitaetsstr. 10, Konstanz 78457, 
            Germany
REFERENCE   3  (bases 1 to 8742)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8742)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2006"; volume: "72"; issue: "12"; 
            pages: "7477-7484"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-FEB-2006) Plant Physiology and Biochemistry, University of 
            Konstanz, Universitaetsstr. 10, Konstanz 78457, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     Name: pPlat-Pds-Mod4.1.
FEATURES             Location/Qualifiers
     source          1..8742
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            134..5825
                     /gene="pdsMod4.1"
                     /label=pdsMod4.1
     regulatory      134..2140
                     /gene="pdsMod4.1"
                     /label=derived from the phytoene desaturase gene
                     /note="derived from the phytoene desaturase gene"
                     /regulatory_class="promoter"
     mRNA            join(2141..2510,2600..2712,2818..3184,3537..3671,3965..4043,
                     4394..4743,4974..5044,5222..5825)
                     /gene="pdsMod4.1"
                     /product="phytoene desaturase"
                     /label=pdsMod4.1 mRNA
     CDS             join(2181..2510,2600..2712,2818..3184,3537..3671,3965..4043,
                     4394..4743,4974..5044,5222..5489)
                     /codon_start=1
                     /gene="pdsMod4.1"
                     /product="phytoene desaturase"
                     /label=pdsMod4.1
                     /protein_id="ABG29431.1"
                     /translation="MQTTMRGQASGSGCTSGRQARGHWSRRSVRERGALRVVAKDYPTP
                     DFQSSDTYQEALSLSTKLRNAPRPAKPLRVVIAGAGLAGLSAAKYLADAGHHPVVLEGR
                     DVLGGKVAAWKDEDGDWYETGLHIFFGAYPNIQNLFKELGIQDRLQWKEHSMIFAMPDA
                     PGEFSRFDFPELPAPWNGIIAILRNNQMLSWPEKIRFRIGLLPAIIFGQRYCEEQDELT
                     VTEWMRKQGVPDRVNEEVFIAMAKALNFINPDDLSMTVVLTALNRFLQEQHGSKMAFLD
                     GAPPERLCQPMVDYFKARGGDLMFNSRVKQIVLNDDKSVKHLALTNGQTVEGDLYISAM
                     PVDIMKILMPDPWASMPYFKQLNGLEGVPVINIHIWFDRKLTTVDHLLFSRSPLLSVYA
                     DMSTTSKEYRDDKKSMLELVFAPAKEWIGRPDEEIIAATMTELERLFPTEVRADQSMAK
                     ILKYKVVKTPLSVYKSTAGREKFRPTQRSPISNFYLAGDYTKQKYRASMEGAVFSGKLV
                     TEAIVEDWSARGVTSSAASRQPALAAAGVVGRVGSSDWARWLQPVGAIAGGCK"
     primer_bind     6387..6403
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6410..6428
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    complement(6437..6544)
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(6557..6575)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6596..6612)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6620..6636)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6644..6674)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6689..6710)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6998..7586)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7760..8617)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(8618..8722)
                     /label=AmpR promoter
ORIGIN
        1 cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
       61 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
      121 tcgccacgtt cgccatcggt gaggggttca agtgccagct cagtcaaaca tcgtgcattc
      181 aggaggcaca gttagaggcg tggttgcaga cgtaggtgag cagttgggtg ctgctgctgc
      241 tgtgatctga tacatgttga aaactgagcg gaatggccat gtattgttgt ttgattactc
      301 atggccatgc actgagcatg cagcaagcca tacccatcca ggagttgtct tcacttcctt
      361 tcccgcgctt gcttgctttc atccgctttc tttccatatg ctgtgttgtg atccctccct
      421 cccctcctcc ctccttaccc tgccccaccc ctggtggtgt gtggccaggt ggctgtgacg
      481 cactgcctgc tgagcgctga cctggtgagc atgagcgcca ggctgggccg agctgcctgg
      541 gtggccacca ctcggctgta tgctgctcag caagaggtgg caggccaggc ccatgtggca
      601 gccatgcacc tggtggcaat cggggatagg gctgcggcag aagcagtgta caggtgggcg
      661 cgggcaggag cagggacagg ggctataggg tccccggcca ggtctaccct gactgctggg
      721 gttaaggcta gggctagggc tagggctagg gctagggcta gggctagggc tagggctagg
      781 gctagggcta gggctagggc tagggctagg gctagggcta gggctagggc taggctaggg
      841 ctagggctaa ggacaagggg tgggggcgaa gggtgggggt ttgcagcagc cagggtcagc
      901 cctgggccaa cagtagctac tgcacatccc cacaatctcc tacaggcagc acaggctaac
      961 gtgcgcggac tgcatcaccg ggtacacagt tgatgacatc ttatcgaagc atctcaccac
     1021 acgacacatc acaattagtt gcaagggttc ctgtcggtgt tgaggtttga gttggtctgt
     1081 atgcaggggc ctgatggaaa aactgtagta gctgagtgag attacccaca gccaagcatg
     1141 ttgttccatg ttgaacaggt tttgtttggt ctgatggaaa aactgttgaa atgcttcctg
     1201 tcatcgttcc gctgtacgcc attgtacgcc gttgactgat ggactgattg tttgactgat
     1261 ggaagaactg tgtagacctc agccccctca gcacattagc atatctcgac acatcagcca
     1321 tgcgcacacc gagttcgcct tgtcatgcgc agccaccctg tagaatgaag tcacgtaagt
     1381 agtggctgcc gcgaggtcgc cttggtattg tctctggctt gatatagcgc tgactacggg
     1441 tggtgacggt gctcgtgcgc tatagatgcc actagaggcc cgcccacctc gcgcctgagc
     1501 cgcaaaaccc ggcggggtcg tcacgatgag ggcatcttac ctcgacgatg taccgacccc
     1561 gggagctgtc ccgctggact ggcggatcgc ggctgcccaa cttgggcact gagcgttggc
     1621 tgtggatcaa catcattggc gccgatgtca gcctgggcca gcaggtgcag cggctgaccg
     1681 gctatggacc ggctatggac cggctacgat agcggatgca gcgttcctcc gcatgcagag
     1741 atgcactgga ccagtaccgg agacggcgcc agcgggatac acggattagt gcgcctgaac
     1801 tgcggtgtgc cccatgaatg accatgccag ccccaagggg cggcattgaa gcggccctag
     1861 agagctagtg gcaaggcagc actagacaac ttatgagcta aggtcaggga gctatgaggc
     1921 tgcaagatta ggggcctgat ttgagttgtt cagtcgcacg tcttcacgac gttacggctg
     1981 ccatcttacc cagtcggcta aagcaggcca ccaccattgg cgtcacgcgt gatcgtcttg
     2041 aaatgacagc tagtccaaac accaggctca gctcgtatgg tttggcgagg gcgtttcgtc
     2101 ggttgaacga cgtgccttta atgaaaattc gtggccatac aattcggcac gaggccaacc
     2161 atccagcatc aactctgaca atgcagacaa caatgcgtgg ccaagccagc ggttcaggat
     2221 gcacttccgg caggcaggct cgcggtcatt ggtcaaggcg ctctgtgcgt gagcgaggtg
     2281 ctctcagggt ggtagccaag gactacccca cgccggattt ccagtcatcg gacacatacc
     2341 aggaggccct gtccctgtcg accaagctgc gaaatgcacc ccggcccgcc aagcctttgc
     2401 gcgttgtcat tgctggggcc ggtctggcag gcctctcggc tgcaaagtac ctggcagatg
     2461 cagggcacca cccagttgtg ctggaggggc gcgacgtact aggcggcaag gtaaggactt
     2521 gcgagtgtcg aatgccacgc cggcccgttc caaacgccca caagctgtcc ggccacctca
     2581 caaactcatt ggcgcacagg tggccgcgtg gaaagacgag gacggtgatt ggtatgagac
     2641 tggccttcac attttctttg gtgcttaccc caacatccag aacctgttca aagagttggg
     2701 catccaagac aggtaggagg tgcacccagt agtgtcaacg cattgcctag caagcgcctc
     2761 agtcaacaag accaccatga gcactgttct tgatgtgggc atgtgcctgc cttgcaggct
     2821 gcagtggaag gagcactcca tgatctttgc catgccagac gcccctgggg agttttctcg
     2881 ctttgacttc cctgagctgc ctgcgccctg gaacggcatc atcgccatct tgcggaacaa
     2941 ccagatgctc agctggcctg agaagatccg gtttcgcatc ggcctgctgc ctgctatcat
     3001 ctttgggcag cgctactgtg aagagcagga tgagctgacc gtgacagagt ggatgcgcaa
     3061 gcagggggtg ccagaccgag tcaatgagga agtgttcatt gccatggcta aggccttgaa
     3121 cttcatcaac cctgacgacc tatccatgac agtagtgctc accgcactga accgtttcct
     3181 gcaggtaagt gttgggccta ggcagtcagg ggtcaggggc ctgcagggcc agcttgcatc
     3241 tgcccgacct tcagggccgc cgcctccctc acaaagcatg caccctccac aacccgcctg
     3301 tctgtctgcc caacaccagc ttatggtcca aatccaccac aaccgcctct caccctcctg
     3361 cccctgcttt cagcctgagc actgcagtcc tgtcaccagc aaatgtgtgg cggctgcact
     3421 tcttgtccct gccgccaatc gctttcagtg tggcctggcc agcttgtgac agtggctaga
     3481 gctccaagct ctgcaaagac tgagacaagc tgtggtttgc attgcctggg ctgcaggagc
     3541 agcatggcag caagatggca ttcttggacg gcgccccacc tgagcgcctc tgtcagccaa
     3601 tggtggacta cttcaaggca cgaggcggcg acctcatgtt caattcccgg gtcaagcaga
     3661 ttgtgctgaa tgtgaggtcc ctggctctca gcctcgccgc ctcctgcaga cgctgcatgc
     3721 catgtgcagt atcaccatgc cacacacgtc ggcatcagct gcaggcctag ctctcgcgcc
     3781 gcagtcacag ttgaacccaa cacatgtgca agtcatcacc ctgtgcagca ggcaggggca
     3841 ggctgggtgc ctgggcctgt catccactgc agccacagtc ttgagtggag ggtacagtca
     3901 ttaactctgc ccatgaggtg tcagagtctt tcatgggtgc tatatggtcg ctcttggcgt
     3961 gcaggacgac aagagcgtca agcacctggc cctcaccaac ggtcagacag ttgagggaga
     4021 cctctacatc tcagccatgc caggtgtgtg cctatggtgc agctgggctg tgtgccccta
     4081 agcagccaca caagcactgg atgccatcac ccattgctgc atgacatagg cacaaggatg
     4141 atagccaacg acatggatga ggcacctggg cacagaggca cacgttgtgg cctgcacagc
     4201 ctgagtcatg aattgagggg ggagagccag gtaggctgta attgtggcac tgacctccac
     4261 aaaaccataa cacaccagat tgactgagtc cagttaggct tgggggcttc ccttcagttt
     4321 gttcagaacc aggatgcttg gctcctccct accatgcgct gacatgtgtg cttgcattga
     4381 tgtggtctcg cagtggacat catgaagatc ctcatgcctg acccctgggc ctccatgccc
     4441 tacttcaagc agctgaacgg cctggagggg gtgcctgtga ttaacatcca catctggttc
     4501 gaccgtaagc tgaccacagt ggaccacctg ctattcagtc ggtcaccgct gctgtctgtg
     4561 tacgctgaca tgagcaccac gtcgaaggaa tatcgtgacg acaagaagag catgcttgag
     4621 ctggtgtttg cgccggccaa ggagtggatt ggccggcctg acgaggagat catcgcagcc
     4681 accatgacgg agctggagcg gctgttcccg acagaggtca gggctgacca gtccatggcc
     4741 aaggtaggcg ggtcaggcac cagccagcac tgccaggcct gcaggttaag aggcttgtga
     4801 ggggtgcagc tctgggcaga tagcagctac agccagctaa cggtgccggg gttgtgagtg
     4861 aatggcgccc actgcctgaa gcaccaccct gctcagcttg ctctgctgtg ccagcattgg
     4921 ctgcctgctg tcgaagtact tgtggtgatg ggtgatggtg gcatgatgtg cagatcttga
     4981 agtacaaggt ggtgaagact ccattgtctg tgtacaagtc cactgctggg cgggagaagt
     5041 tcaggtgagc ctcgcgacag ccgacagtgg aatgcactgc agcccttgtg gctgcctgac
     5101 tggctttctt gtcagccgct ctctcgtgac cataactgat gctgccgtac cttatctgct
     5161 gctgctgctg tcatgctggt gctaatggtg gtgccccggt gctgctgctg ctgtgctgca
     5221 gacccactca gcgctcccca atctccaact tctaccttgc gggtgattac accaagcaga
     5281 agtaccgcgc ctccatggag ggtgcagtgt tctccggcaa gctggtcact gaggccattg
     5341 ttgaggactg gagcgcgcgg ggtgtgacca gcagcgctgc cagccgccag cctgccttgg
     5401 ctgcagccgg tgtggttggc agggtcggct cgagtgattg ggcgcgttgg ttgcagccag
     5461 ttggtgcaat agccggtgga tgtaagtgat gaaagaatgg cacctggtag atgaggcagc
     5521 atttatagaa atgcagcacc ctacgctagc ctaatgcttg tggtgtatac tattgacctc
     5581 gggagttgag gctcgcagcc attgtgtgcg tatcgcatgg gatggtgcat cgctgctcaa
     5641 tgccgacaac attgggatgg cctagaactt attgctaggt agtgtgacct ggacaagaga
     5701 ttgggtatgg gtctggtgag gaagggcagg cactatatgg gcgagcattc tgagaggttg
     5761 ggcaggcgtg ctgtagtcct gcgatgagtt ggagtgtctg aacggcagta acggatatgg
     5821 ggtcacacgg tttacacgaa cattgctgaa atcgaggatc atcacgaagc gtgcaagaaa
     5881 cgtcatgcaa catgcaaaca agccaaaatt caaacgatgg ctttccccgt caagctctaa
     5941 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     6001 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     6061 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     6121 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     6181 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     6241 caatttccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     6301 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     6361 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattgt aatacgactc
     6421 actatagggc gaattgggta ccgggccccc cctcgaggtc gacggtatcg ataagcttga
     6481 tatcgaattc ctgcagcccg ggggatccac tagttctaga gcggccgcca ccgcggtgga
     6541 gctccagctt ttgttccctt tagtgagggt taatttcgag cttggcgtaa tcatggtcat
     6601 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     6661 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     6721 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     6781 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     6841 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     6901 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     6961 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     7021 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     7081 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     7141 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     7201 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     7261 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     7321 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     7381 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     7441 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     7501 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     7561 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     7621 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     7681 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     7741 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     7801 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     7861 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     7921 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     7981 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     8041 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     8101 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     8161 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     8221 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     8281 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     8341 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     8401 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     8461 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     8521 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     8581 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     8641 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     8701 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gc
//
