LOCUS       40924_34465        4196 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Promoterless PhiYFP reporter vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Evrogen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4196)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Can be used to monitor transcription from a promoter inserted into 
            the MCS.
FEATURES             Location/Qualifiers
     source          1..4196
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    9..89
                     /label=MCS
                     /note="multiple cloning site"
     CDS             97..798
                     /codon_start=1
                     /label=PhiYFP
                     /note="Phialidium yellow fluorescent proteim"
                     /translation="MSSGALLFHGKIPYVVEMEGNVDGHTFSIRGKGYGDASVGKVDAQ
                     FICTTGDVPVPWSTLVTTLTYGAQCFAKYGPELKDFYKSCMPDGYVQERTITFEGDGNF
                     KTRAEVTFENGSVYNRVKLNGQGFKKDGHVLGKNLEFNFTPHCLYIWGDQANHGLKSAF
                     KICHEITGSKGDFIVADHTQMNTPIGGGPVHVPEYHHMSYHVKLSKDVTDHRDNMSLKE
                     TVRAVDCRKTYL"
     polyA_signal    984..1105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1112..1567)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1594..1698
                     /label=AmpR promoter
     promoter        1700..2057
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2092..2883
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    3118..3165
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3494..4082
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattac tagcgctacc ggactcagat ctcgagctca agcttcgaat tctgcagtcg
       61 acggtaccgc gggcccggga tccaccggtc gccaccatga gcagcggcgc cctgctgttc
      121 cacggcaaga tcccctacgt ggtggagatg gagggcaatg tggatggcca caccttcagc
      181 atccgcggca agggctacgg cgatgccagc gtgggcaagg tggatgccca gttcatctgc
      241 accaccggcg atgtgcccgt gccctggagc accctggtga ccaccctgac ctacggcgcc
      301 cagtgcttcg ccaagtacgg ccccgagctg aaggatttct acaagagctg catgcccgat
      361 ggctacgtgc aggagcgcac catcaccttc gagggcgatg gcaatttcaa gacccgcgcc
      421 gaggtgacct tcgagaatgg cagcgtgtac aatcgcgtga agctgaatgg ccagggcttc
      481 aagaaggatg gccacgtgct gggcaagaat ctggagttca atttcacccc ccactgcctg
      541 tacatctggg gcgatcaggc caatcacggc ctgaagagcg ccttcaagat ctgccacgag
      601 atcaccggca gcaagggcga tttcatcgtg gccgatcaca cccagatgaa tacccccatc
      661 ggcggcggcc ccgtgcacgt gcccgagtac caccacatga gctaccacgt gaagctgagc
      721 aaggatgtga ccgatcaccg cgataatatg agcctgaagg agaccgtgcg cgccgtggat
      781 tgccgcaaga cctacctgtg aagcttaatt agctgagctt ggactcctgt tgatagatcc
      841 agtaatgacc tcagaactcc atctggccgc gactctagat cataatcagc cataccacat
      901 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
      961 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     1021 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     1081 tgtccaaact catcaatgta tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa
     1141 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     1201 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     1261 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     1321 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     1381 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     1441 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     1501 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     1561 ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     1621 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     1681 tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag
     1741 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     1801 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     1861 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     1921 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     1981 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     2041 gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa
     2101 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     2161 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     2221 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag
     2281 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     2341 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     2401 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     2461 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     2521 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     2581 gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat
     2641 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     2701 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     2761 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     2821 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     2881 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     2941 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     3001 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta
     3061 gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg
     3121 caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc
     3181 ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc
     3241 gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag
     3301 ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg
     3361 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     3421 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     3481 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     3541 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     3601 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     3661 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     3721 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     3781 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     3841 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     3901 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     3961 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     4021 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     4081 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     4141 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcat
//
