LOCUS       40924_34446        4095 bp DNA     circular SYN 29-DEC-2023
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4095
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    461..517
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     CDS             1093..1464
                     /label=BleoR
                     /note="antibiotic-binding protein"
     promoter        complement(1719..1737)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(1995..2583)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2757..3614)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3615..3719)
                     /label=AmpR promoter
     promoter        4065..4083
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gggctgcagg acgcaatgga ggattatcac cgcaaaaatg aacttcgaaa aaaactttcg
       61 agcgaccatg gaaaaggagg atcagattca gattacaaca gtggattgct ctggtagcaa
      121 atatcttctg ctagattggc tcatggtcgg ttttggacgt tcgaagctca ccgtcaaaag
      181 aaacaaaaga gaagaatgac gtcttcgtga cgtagaatct acgactgtac tcggatctgg
      241 gaaatgaatt gactcacggt cttcttcgag tcctgttaca ggcccttggt ccgaaccccc
      301 acacgatttt tgcaccaaag atttgcttca atttgctgga tgttttgact gcaagatcag
      361 ctggcctagc aagagtgctc gtgttgcttc gtcgggaatc cctacgaatt tcagttctgc
      421 acaaatttgt ctgccgtttc gagaattcga tatcatcgac taattcgagc tcggtacccg
      481 gggatcctct agagtcgacc tgcaggcatg caagcttcag aagcgtgcta tcgaactcaa
      541 ccagggacgt gcggcacaaa tgggcatcct tgctctcatg gtgcacgaac agttgggagt
      601 ctctatcctt ccttaaaaat ttaattttca ttagttgcag tcactccgct ttggtttcac
      661 agtcaggaat aacactagct cgtcttcacc atggatgcca atctcgccta ttcatggtgt
      721 ataaaagttc aacatccaaa gctagaactt ttggaaagag aaagaatatc cgaatagggc
      781 acggcgtgcc gtattgttgg agtggactag cagaaagtga ggaaggcaca ggatgagttt
      841 tctcgagaca taccttcagc gtcgtcttca ctgtcacagt caactgacag taatcgttga
      901 tccggagaga ttcaaaattc aatctgtttg gacctggata agacacaaga gcgacatcct
      961 gacatgaacg ccgtaaacag caaatcctgg ttgaacacgt atccttttgg gggcctccgc
     1021 tacgacgctc gctccagctg gggcttcctt actatacaca gcgcgcatat ttcacggttg
     1081 ccagatgtca agatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc
     1141 gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac
     1201 gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag
     1261 gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc
     1321 gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag
     1381 atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg
     1441 cacttcgtgg ccgaggagca ggactgaacc ttccttaaaa atttaatttt cattagttgc
     1501 agtcactccg ctttggtttc acagtcagga ataacactag ctcgtcttca ccatggatgc
     1561 caatctcgcc tattcatggt gtataaaagt tcaacatcca aagctagaac ttttggaaag
     1621 agaaagaata tccgaatagg gcacggcgtg ccgtattgtt ggagtggact agcagaaagt
     1681 gaggaaggca caggatgagt tttctcgagg ccggtctccc tatagtgagt cgtattaatt
     1741 tcgataagcc aggttaacct gcattaatga atcggccaac gcgcggggag aggcggtttg
     1801 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     1861 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     1921 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     1981 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     2041 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     2101 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     2161 ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg
     2221 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     2281 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     2341 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     2401 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     2461 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     2521 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     2581 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     2641 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     2701 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     2761 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     2821 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     2881 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     2941 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     3001 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     3061 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     3121 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     3181 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     3241 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     3301 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     3361 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     3421 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     3481 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     3541 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     3601 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     3661 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     3721 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     3781 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     3841 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     3901 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
     3961 tatgcggcat cagagcagat tgtactgaga gtgcaccata tggacatatt gtcgttagaa
     4021 cgcggctaca attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat
     4081 agaaccagat ccccc
//
