LOCUS       40924_34441        3860 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pPha-NR, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Stork S, Moog D, Przyborski JM, Wilhelmi I, Zauner S, Maier UG.
  TITLE     Distribution of the SELMA Translocon in Secondary Plastids of Red 
            Algal Origin and Predicted Uncoupling of Ubiquitin-Dependent 
            Translocation from Degradation
  JOURNAL   Eukaryotic Cell 11 (12), 1472-1481 (2012)
  PUBMED    23042132
REFERENCE   2  (bases 1 to 3860)
  AUTHORS   Zauner S, Hempel F.
  TITLE     Inducible shuttle vector
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 3860)
  AUTHORS   Zauner S, Hempel F.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JUN-2011) Cell Biology, Philipps-University Marburg, 
            Karl-von-Frisch Str. 8, Marburg 35032, Germany
REFERENCE   4  (bases 1 to 3860)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 3860)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eukaryotic 
            Cell"; date: "2012"; volume: "11"; issue: "12"; pages: "1472-1481"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (24-JUN-2011) Cell Biology, Philipps-University Marburg, 
            Karl-von-Frisch Str. 8, Marburg 35032, Germany"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3860
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..422
                     /note="nitrate reductase; derived from Phaeodactylum 
                     tricornutum"
                     /regulatory_class="promoter"
     misc_feature    441..497
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     regulatory      498..770
                     /note="nitrate reductase; derived from Phaeodactylum 
                     tricornutum"
                     /regulatory_class="terminator"
     regulatory      771..956
                     /note="fcpB; derived from Phaeodactylum tricornutum"
                     /regulatory_class="promoter"
     CDS             957..1328
                     /label=BleoR
                     /note="antibiotic-binding protein"
     regulatory      1331..1573
                     /note="fcpA; derived from Phaeodactylum tricornutum"
                     /regulatory_class="terminator"
     promoter        complement(1583..1601)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(1859..2447)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2621..3478)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3479..3583)
                     /label=AmpR promoter
ORIGIN
        1 catatgcgga agtgactgta aacgagaagt gcacgaagcc ttttcttgtg acgtcacaaa
       61 ccgaacagcc ctacgtggcg tgcgactttc cggcactttg gatatcgttg ccctatatgt
      121 attgggtgta tacatgtcgt attccaacta cgacagaaaa cagtcagtta cgggtattcg
      181 aacttccggc acctgcagcg aggagttttg tgtccgtcgc acatcctccg tgctttcggc
      241 acctacgtag gcggcaaact tcctgccttc cgcccgcctt gcggcattcc ggctccgggc
      301 cagaattgcc cgggtgttca caattttgcc tcctcacgaa aaaacgttct actttgtatt
      361 ttggtcgggt ttcggatcct tccagcaacc attctcattc aaagtcacca cttgtgcgaa
      421 cggaattcga tatcatcgac taattcgagc tcggtacccg gggatcctct agagtcgacc
      481 tgcaggcatg caagcttcaa tttactttaa gactagcgca atttttttct tacattagat
      541 gaacataaaa cgacgatgag gatcactgtt agggaaacga tgaactacgc tgaggacttc
      601 ctattattcg tcctttgatg gcgtggttgt tctttggttt gcggccgagc gcaacgacca
      661 aggcctgtac caaaaacgaa gttgataatc agatatttga gttgtaacaa gaaactaaag
      721 cattatttta gcagtagcac ggatcaggat ctaaaatgaa cgcatccgga gagattcaaa
      781 attcaatctg tttggacctg gataagacac aagagcgaca tcctgacatg aacgccgtaa
      841 acagcaaatc ctggttgaac acgtatcctt ttgggggcct ccgctacgac gctcgctcca
      901 gctggggctt ccttactata cacagcgcgc atatttcacg gttgccagat gtcaagatgg
      961 ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt
     1021 tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg
     1081 tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca
     1141 ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg
     1201 tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt
     1261 gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg
     1321 agcaggactg aaccttcctt aaaaatttaa ttttcattag ttgcagtcac tccgctttgg
     1381 tttcacagtc aggaataaca ctagctcgtc ttcaccatgg atgccaatct cgcctattca
     1441 tggtgtataa aagttcaaca tccaaagcta gaacttttgg aaagagaaag aatatccgaa
     1501 tagggcacgg cgtgccgtat tgttggagtg gactagcaga aagtgaggaa ggcacaggat
     1561 gagttttctc gaggccggtc tccctatagt gagtcgtatt aatttcgata agccaggtta
     1621 acctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     1681 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     1741 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     1801 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     1861 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     1921 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     1981 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     2041 tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     2101 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     2161 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     2221 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     2281 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     2341 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     2401 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     2461 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     2521 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     2581 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     2641 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     2701 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     2761 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     2821 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     2881 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     2941 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     3001 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     3061 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     3121 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     3181 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     3241 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     3301 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     3361 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     3421 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     3481 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     3541 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     3601 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     3661 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     3721 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     3781 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc
     3841 agattgtact gagagtgcac
//
