LOCUS       V004115                 4461 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004115
VERSION     V004115
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Krom RJ, Bhargava P, Lobritz MA, Collins JJ.
  TITLE     Engineered Phagemids for Nonlytic, Targeted Antibacterial Therapies
  JOURNAL   Nano Lett. 15 (7), 4808-4813 (2015)
   PUBMED   26044909
REFERENCE   2  (bases 1 to 4461)
  AUTHORS   Krom RJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUN-2015) Molecular Medicine, Boston University, 72
            East Concord St, Boston, MA 02118, USA
REFERENCE   3  (bases 1 to 4461)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4461)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nano
            Lett."; date: "2015"; volume: "15"; issue: "7"; pages: "4808-4813"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-JUN-2015) Molecular Medicine, Boston University, 72 East Concord
            St, Boston, MA 02118, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4461
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        31..104
                     /label="PLtetO-1 promoter"
                     /note="modified phage lambda PL promoter with tet operator
                     sites (Lutz and Bujard, 1997)"
     CDS             142..264
                     /codon_start=1
                     /product="cecropin PR-39"
                     /label="cecropin PR-39"
                     /protein_id="AKQ98660.1"
                     /translation="MRRRPRPPYLPRPRPPPFFPPRLPPRIPPGFPPRFPPRFP"
     CDS             313..435
                     /codon_start=1
                     /product="cecropin PR-39"
                     /label="cecropin PR-39"
                     /protein_id="AKQ98661.1"
                     /translation="MRRRPRPPYLPRPRPPPFFPPRLPPRIPPGFPPRFPPRFP"
     terminator      456..550
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     regulatory      631..704
                     /label="PLtet0"
                     /note="PLtet0"
                     /regulatory_class="promoter"
     promoter        631..704
                     /label="PLtetO-1 promoter"
                     /note="modified phage lambda PL promoter with tet operator
                     sites (Lutz and Bujard, 1997)"
     protein_bind    631..649
                     /gene="tetO"
                     /label="tet operator"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    656..674
                     /gene="tetO"
                     /label="tet operator"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             730..738
                     /label="Shine-Dalgarno sequence"
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to a
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             745..804
                     /codon_start=1
                     /product="apidaecin"
                     /label="apidaecin"
                     /protein_id="AKQ98662.1"
                     /translation="MGNNRPVYIPQPRPPHPRI"
     RBS             843..851
                     /label="Shine-Dalgarno sequence"
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to a
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             857..916
                     /codon_start=1
                     /product="apidaecin"
                     /label="apidaecin"
                     /protein_id="AKQ98663.1"
                     /translation="MGNNRPVYIPQPRPPHPRI"
     regulatory      923..1045
                     /label="T0"
                     /note="T0"
                     /regulatory_class="terminator"
     terminator      937..1031
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     regulatory      1064..1137
                     /label="PLtet0"
                     /note="PLtet0"
                     /regulatory_class="promoter"
     promoter        1064..1137
                     /label="PLtetO-1 promoter"
                     /note="modified phage lambda PL promoter with tet operator
                     sites (Lutz and Bujard, 1997)"
     protein_bind    1064..1082
                     /gene="tetO"
                     /label="tet operator"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1089..1107
                     /gene="tetO"
                     /label="tet operator"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             1170..1478
                     /gene="parE"
                     /label="Toxin ParE"
                     /note="Toxin ParE from Escherichia coli. Accession#:
                     Q79EC5"
     CDS             1533..1841
                     /gene="parE"
                     /label="Toxin ParE"
                     /note="Toxin ParE from Escherichia coli. Accession#:
                     Q79EC5"
     regulatory      1851..1973
                     /label="T0"
                     /note="T0"
                     /regulatory_class="terminator"
     terminator      1865..1959
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     rep_origin      2028..2455
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     terminator      2480..2566
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(2730..3318)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     terminator      complement(3406..3500)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(3534..4325)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 gacgtctgtg caagtactac tgttctgcag tccctatcag tgatagagat tgacatccct
       61 atcagtgata gagatactga gcacatcagc aggacgcact gaccggtacc ctataagtta
      121 cacaacataa ggaggaatat aatgcgtcgt cgtccgcgtc cgccgtacct gccgcgtccg
      181 cgtccgccgc cgttcttccc gccgcgtctg ccgccgcgta tcccgccggg tttcccgccg
      241 cgtttcccgc cgcgtttccc gtaaccgcgg agtatttgta cattctatca aagtatcata
      301 aggaggagaa taatgcgtcg tcgtccgcgt ccgccgtacc tgccgcgtcc gcgtccgccg
      361 ccgttcttcc cgccgcgtct gccgccgcgt atcccgccgg gtttcccgcc gcgtttcccg
      421 ccgcgtttcc cgtaaggatc ccgagaattg gcttggactc ctgttgatag atccagtaat
      481 gacctcagaa ctccatctgg atttgttcag aacgctcggt tgccgccggg cgttttttat
      541 tggtgagaat ccaagcagta gtcagaattc gatacccagc tgggtggagt gcaccaagga
      601 gcatgcgaag gaaacgtttc gcagaagctt tccctatcag tgatagagat tgacatccct
      661 atcagtgata gagatactga gcacatcagc aggacgcact gaccggtacc caacagggat
      721 tcgttaattt aaggaggtct ccaaatgggc aacaaccgcc cggtgtatat tccgcagccg
      781 cgcccgccgc atccgcgcat ttaaccgcgg agtatttgta cagaggtaca actcaactaa
      841 tataaggagg ttcaaaatgg gcaacaaccg cccggtgtat attccgcagc cgcgcccgcc
      901 gcatccgcgc atttaaggat cccgagaatt ggcttggact cctgttgata gatccagtaa
      961 tgacctcaga actccatctg gatttgttca gaacgctcgg ttgccgccgg gcgtttttta
     1021 ttggtgagaa tccaagcagt agtcagagct cggactgctt aagtccctat cagtgataga
     1081 gattgacatc cctatcagtg atagagatac tgagcacatc agcaggacgc actgaccggt
     1141 acctcacaat caaatctaag gagttacaaa tgaccgcgta tattctgacc gctgaagcgg
     1201 aagcggatct gcgcggcatt attcgctata cccgccgcga atggggcgcg gcgcaggtgc
     1261 gccgctatat tgcgaaactg gaacagggca ttgcgcgcct ggcggcgggc gaaggcccgt
     1321 ttaaagatat gagcgaactg tttccggcgc tgcgcatggc gcgctgcgaa catcattatg
     1381 tgttttgcct gccgcgcgcg ggcgaaccgg cgctggtggt ggcgattctg catgaacgca
     1441 tggatctgat gacccgcctg gcggatcgcc tgaaaggcta accgcggagt atttgtacat
     1501 acactatatt aacctaaaaa ggagcgtaac gaatgaccgc gtatattctg accgctgaag
     1561 cggaagcgga tctgcgcggc attattcgct atacccgccg cgaatggggc gcggcgcagg
     1621 tgcgccgcta tattgcgaaa ctggaacagg gcattgcgcg cctggcggcg ggcgaaggcc
     1681 cgtttaaaga tatgagcgaa ctgtttccgg cgctgcgcat ggcgcgctgc gaacatcatt
     1741 atgtgttttg cctgccgcgc gcgggcgaac cggcgctggt ggtggcgatt ctgcatgaac
     1801 gcatggatct gatgacccgc ctggcggatc gcctgaaagg ctaaggatcc cgagaattgg
     1861 cttggactcc tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga
     1921 acgctcggtt gccgccgggc gttttttatt ggtgagaatc caagcagtag tcacaattgt
     1981 cccccggcgc cagggttgat atctatcgcc ctagggaccg tctcgagagc gccctgtagc
     2041 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     2101 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     2161 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     2221 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     2281 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     2341 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     2401 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaaata
     2461 ttaatccaac gcgtggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     2521 ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgccctagac
     2581 ttaggcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     2641 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     2701 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     2761 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     2821 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     2881 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     2941 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtctgcacga accccccgtt
     3001 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     3061 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     3121 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     3181 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     3241 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     3301 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     3361 aacgaaaact cacgttaagg gattttggtc atggctagtg cttggattct caccaataaa
     3421 aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac
     3481 tggatctatc aacaggagtc caagccaatt ctcgaacccc agagtcccgc tcagaagaac
     3541 tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc
     3601 acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac
     3661 gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag
     3721 cggccatttt ccaccatgat attcggcaag caggcatcgc cgtgggtcac gacgagatcc
     3781 tcgccgtcgg gcatacgcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga
     3841 tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc
     3901 tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc
     3961 cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg
     4021 agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg
     4081 tcgagcacag ccgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg
     4141 tcttgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgaccc
     4201 tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca
     4261 tagccgaata gcctctccac ccaagccgcc ggagaacctg cgtgcaatcc atcttgttca
     4321 atcatgcgaa acgatcctca tcctgtctct tgatcagatc ttgatcccct gcgccatcag
     4381 atccttggcg gcaagaaagc catccagttt actttgcagg gcttcccaac cttaccagag
     4441 ggcggcccaa ctggcaattc c
//
