LOCUS       40924_34416        4263 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPh110, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Krom RJ, Bhargava P, Lobritz MA, Collins JJ.
  TITLE     Engineered Phagemids for Nonlytic, Targeted Antibacterial Therapies
  JOURNAL   Nano Lett. 15 (7), 4808-4813 (2015)
  PUBMED    26044909
REFERENCE   2  (bases 1 to 4263)
  AUTHORS   Krom RJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUN-2015) Molecular Medicine, Boston University, 72 
            East Concord St, Boston, MA 02118, USA
REFERENCE   3  (bases 1 to 4263)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4263)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nano 
            Lett."; date: "2015"; volume: "15"; issue: "7"; pages: "4808-4813"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-JUN-2015) Molecular Medicine, Boston University, 72 East Concord
            St, Boston, MA 02118, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        31..104
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator
                     sites (Lutz and Bujard, 1997)"
     CDS             142..264
                     /codon_start=1
                     /product="cecropin PR-39"
                     /label=cecropin PR-39
                     /protein_id="AKQ98654.1"
                     /translation="MRRRPRPPYLPRPRPPPFFPPRLPPRIPPGFPPRFPPRFP"
     CDS             313..435
                     /codon_start=1
                     /product="cecropin PR-39"
                     /label=cecropin PR-39
                     /protein_id="AKQ98655.1"
                     /translation="MRRRPRPPYLPRPRPPPFFPPRLPPRIPPGFPPRFPPRFP"
     terminator      456..550
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     regulatory      631..704
                     /label=PLtet0
                     /note="PLtet0"
                     /regulatory_class="promoter"
     promoter        631..704
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator
                     sites (Lutz and Bujard, 1997)"
     protein_bind    631..649
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    656..674
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             730..738
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             745..804
                     /codon_start=1
                     /product="apidaecin"
                     /label=apidaecin
                     /protein_id="AKQ98656.1"
                     /translation="MGNNRPVYIPQPRPPHPRI"
     RBS             843..851
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             857..916
                     /codon_start=1
                     /product="apidaecin"
                     /label=apidaecin
                     /protein_id="AKQ98657.1"
                     /translation="MGNNRPVYIPQPRPPHPRI"
     regulatory      923..1045
                     /label=T0
                     /note="T0"
                     /regulatory_class="terminator"
     terminator      937..1031
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     regulatory      1064..1137
                     /label=PLtet0
                     /note="PLtet0"
                     /regulatory_class="promoter"
     promoter        1064..1137
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator
                     sites (Lutz and Bujard, 1997)"
     protein_bind    1064..1082
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1089..1107
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             1175..1384
                     /codon_start=1
                     /product="YeeV'"
                     /label=YeeV'
                     /protein_id="AKQ98658.1"
                     /translation="MNTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFAD
                     ERVIEQHIEAGISLCDAVNFLVEK"
     CDS             1437..1646
                     /codon_start=1
                     /product="YeeV'"
                     /label=YeeV'
                     /protein_id="AKQ98659.1"
                     /translation="MNTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFAD
                     ERVIEQHIEAGISLCDAVNFLVEK"
     regulatory      1653..1775
                     /label=T0
                     /note="T0"
                     /regulatory_class="terminator"
     terminator      1667..1761
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      1830..2257
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     terminator      2282..2368
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(2532..3120)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(3208..3302)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3336..4127)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 gacgtctgtg caagtactac tgttctgcag tccctatcag tgatagagat tgacatccct
       61 atcagtgata gagatactga gcacatcagc aggacgcact gaccggtacc ctataagtta
      121 cacaacataa ggaggaatat aatgcgtcgt cgtccgcgtc cgccgtacct gccgcgtccg
      181 cgtccgccgc cgttcttccc gccgcgtctg ccgccgcgta tcccgccggg tttcccgccg
      241 cgtttcccgc cgcgtttccc gtaaccgcgg agtatttgta cattctatca aagtatcata
      301 aggaggagaa taatgcgtcg tcgtccgcgt ccgccgtacc tgccgcgtcc gcgtccgccg
      361 ccgttcttcc cgccgcgtct gccgccgcgt atcccgccgg gtttcccgcc gcgtttcccg
      421 ccgcgtttcc cgtaaggatc ccgagaattg gcttggactc ctgttgatag atccagtaat
      481 gacctcagaa ctccatctgg atttgttcag aacgctcggt tgccgccggg cgttttttat
      541 tggtgagaat ccaagcagta gtcagaattc gatacccagc tgggtggagt gcaccaagga
      601 gcatgcgaag gaaacgtttc gcagaagctt tccctatcag tgatagagat tgacatccct
      661 atcagtgata gagatactga gcacatcagc aggacgcact gaccggtacc caacagggat
      721 tcgttaattt aaggaggtct ccaaatgggc aacaaccgcc cggtgtatat tccgcagccg
      781 cgcccgccgc atccgcgcat ttaaccgcgg agtatttgta cagaggtaca actcaactaa
      841 tataaggagg ttcaaaatgg gcaacaaccg cccggtgtat attccgcagc cgcgcccgcc
      901 gcatccgcgc atttaaggat cccgagaatt ggcttggact cctgttgata gatccagtaa
      961 tgacctcaga actccatctg gatttgttca gaacgctcgg ttgccgccgg gcgtttttta
     1021 ttggtgagaa tccaagcagt agtcagagct cggactgctt aagtccctat cagtgataga
     1081 gattgacatc cctatcagtg atagagatac tgagcacatc agcaggacgc actgaccggt
     1141 acccaagaag atagaggagg taatatacaa gagaatgaac accctgccgg atacccatgt
     1201 gcgcgaagcg agccgctgcc cgagcccggt gaccatttgg cagaccctgc tgacccgcct
     1261 gctggatcag cattatggcc tgaccctgaa cgataccccg tttgcggatg aacgtgtgat
     1321 tgaacagcat attgaagcgg gcattagcct gtgcgatgcg gtgaactttc tggtggaaaa
     1381 ataaccgcgg agtatttgta caagcctaac ggaagtcgac tagtaaggag aactaaatga
     1441 acaccctgcc ggatacccat gtgcgcgaag cgagccgctg cccgagcccg gtgaccattt
     1501 ggcagaccct gctgacccgc ctgctggatc agcattatgg cctgaccctg aacgataccc
     1561 cgtttgcgga tgaacgtgtg attgaacagc atattgaagc gggcattagc ctgtgcgatg
     1621 cggtgaactt tctggtggaa aaataaggat cccgagaatt ggcttggact cctgttgata
     1681 gatccagtaa tgacctcaga actccatctg gatttgttca gaacgctcgg ttgccgccgg
     1741 gcgtttttta ttggtgagaa tccaagcagt agtcacaatt gtcccccggc gccagggttg
     1801 atatctatcg ccctagggac cgtctcgaga gcgccctgta gcggcgcatt aagcgcggcg
     1861 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     1921 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     1981 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     2041 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     2101 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     2161 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     2221 aaaaatgagc tgatttaaca aaaatttaac gcgaattaaa tattaatcca acgcgtggca
     2281 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     2341 ggtgaacgct ctcctgagta ggacaaatcc gccgccctag acttaggcgt tcggctgcgg
     2401 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     2461 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     2521 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     2581 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     2641 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     2701 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     2761 gtcgttcgct ccaagctggg ctgtctgcac gaaccccccg ttcagcccga ccgctgcgcc
     2821 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     2881 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     2941 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     3001 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     3061 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     3121 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     3181 gggattttgg tcatggctag tgcttggatt ctcaccaata aaaaacgccc ggcggcaacc
     3241 gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag
     3301 tccaagccaa ttctcgaacc ccagagtccc gctcagaaga actcgtcaag aaggcgatag
     3361 aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc
     3421 cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg
     3481 tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg
     3541 atattcggca agcaggcatc gccgtgggtc acgacgagat cctcgccgtc gggcatacgc
     3601 gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca
     3661 tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct
     3721 tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc
     3781 atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact
     3841 tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agccgcgcaa
     3901 ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcttgcag ttcattcagg
     3961 gcaccggaca ggtcggtctt gacaaaaaga accgggcgac cctgcgctga cagccggaac
     4021 acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc
     4081 acccaagccg ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct
     4141 catcctgtct cttgatcaga tcttgatccc ctgcgccatc agatccttgg cggcaagaaa
     4201 gccatccagt ttactttgca gggcttccca accttaccag agggcggccc aactggcaat
     4261 tcc
//
