LOCUS       40924_35848        5638 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPZP500, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5638)
  AUTHORS   Ali MA, Shah KH, Bohlmann H.
  TITLE     pMAA-Red: Binary cloning vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5638)
  AUTHORS   Ali MA, Shah KH, Bohlmann H.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAY-2012) Department of Crop Sciences, Division of 
            Plant Protection, University of Natural Resources and Life Sciences,
            Konrad Lorenz Strasse 24, Tulln, Lower Austria 3430, Austria
REFERENCE   3  (bases 1 to 5638)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5638)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-MAY-2012) Department of Crop Sciences, Division of Plant 
            Protection, University of Natural Resources and Life Sciences, 
            Konrad Lorenz Strasse 24, Tulln, Lower Austria 3430, Austria"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5638
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             404..1030
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             1462..2532
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QATADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      2601..2795
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      complement(3148..3736)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3983..4771)
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    5294..5318
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    5387..5443
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     misc_feature    5500..5524
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 agtactttaa agtactttaa agtactttaa agtactttga tccaacccct ccgctgctat
       61 agtgcagtcg gctccggaga atgaacgcca agaggaacaa gcatgaaacc gtaccaggac
      121 ggccaggacg aaccgttttt cattaccgaa gagatcgagg cggagatgat cgcgtcgggt
      181 acgtgttcga gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc ctggccggtt
      241 tgtctgatgc caagctggcg gcctggccgg ccagcttggc cgctgaagaa accgagcgcc
      301 gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt
      361 atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt
      421 acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag cccgcgccct
      481 gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca gtgcccgcga
      541 ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat
      601 tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg gagcgcccca
      661 ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca
      721 gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta agcagcgcat
      781 tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac
      841 gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca ttcttgagtc
      901 ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa ccgttcttga
      961 atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc
     1021 aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa cacgctaagt
     1081 gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct ggcagacacg
     1141 ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa gctgaagatg
     1201 tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat cgcgcagcta
     1261 ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta aaggaggcgg
     1321 catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt gtggaggaac
     1381 gggcggttgg ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg gcactggaac
     1441 ccccaagccc gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt acaaatcggc
     1501 gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa
     1561 cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag caaccgctga tcgaatccgc
     1621 aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc
     1681 gacgagcaac cagatttttt cgttccgatg ctctatgacg tgggcacccg cgatagtcgc
     1741 agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg
     1801 atccgctacg agcttccaga cgggcacgta gaggtttccg cagggccggc cggcatggcc
     1861 agtgtgtggg attacgacct ggtactgatg gcggtttccc atctaaccga atccatgaac
     1921 cgataccggg aagggaaggg agacaaaccc ggccgcgtgt tccgtccaca cgttgcggac
     1981 gtactcaagt tctgccggcg agccgatggc ggaaagcaga aagacgacct ggtagaaacc
     2041 tgcattcggt taaacaccac gcacgttgcc atgcagcgta cgaagaaggc caagaacggc
     2101 cgcctggtga cggtatccga gggtgaagcc ttgattagcc gctacaagat cgtaaagagc
     2161 gaaaccgggc ggccggagta catcgagatc gagctagctg attggatgta ccgcgagatc
     2221 acagaaggca agaacccgga cgtgctgacg gttcaccccg attacttttt gatcgatccc
     2281 ggcatcggcc gttttctcta ccgcctggca cgccgcgccg caggcaaggc agaagccaga
     2341 tggttgttca agacgatcta cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt
     2401 ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg agtacgattt gaaggaggag
     2461 gcggggcagg ctggcccgat cctagtcatg cgctaccgca acctgatcga gggcgaagca
     2521 tccgccggtt cctaatgtac ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa
     2581 ggtcgaaaag gtctctttcc tgtggatagc acgtacattg ggaacccaaa gccgtacatt
     2641 gggaaccgga acccgtacat tgggaaccca aagccgtaca ttgggaaccg gtcacacatg
     2701 taagtgactg atataaaaga gaaaaaaggc gatttttccg cctaaaactc tttaaaactt
     2761 attaaaactc ttaaaacccg cctggcctgt gcataactgt ctggccagcg cacagccgaa
     2821 gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt
     2881 cggcctctcg agtgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     2941 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     3001 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     3061 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     3121 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     3181 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     3241 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     3301 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     3361 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     3421 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     3481 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     3541 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     3601 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     3661 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     3721 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     3781 cgaaaactca cgttaaggga ttttggtcat gcatgatata tctcccaatt tgtgtagggc
     3841 ttattatgca cgcttaaaaa taataaaagc agacttgacc tgatagtttg gctgtgagca
     3901 attatgtgct tagtgcatct aatcgcttga gttaacgccg gcgaagcggc gtcggcttga
     3961 acgaatttct agctagacat tatttgccga ctaccttggt gatctcgcct ttcacgtagt
     4021 ggacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat
     4081 aagcctgtct agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc
     4141 agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg
     4201 acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg
     4261 ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct
     4321 ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca
     4381 gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt
     4441 ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa
     4501 caatggtgac ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca
     4561 aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca
     4621 gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta
     4681 cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag
     4741 tcgatacttc ggcgatcacc gcttccccca tgatgtttaa ctatgtttta gggcgactgc
     4801 cctgctgggt aacatcgttg ctgctcccat aacatcaaac atcgacccac ggcgtaacgc
     4861 gcttgctgct tggatgcccg aggcatagac tgtaccccaa aaaaacaagt cataacaagc
     4921 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     4981 gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
     5041 ggttcgtgcc cgaattgatc acaggcagca acgctctgtc atcgttacaa tcaacatgct
     5101 accctccgcg agatcatccg tgtttcaaac ccggcagctt agttgccgtt cttccgaata
     5161 gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc tctcccgctg acgccgtccc
     5221 ggactgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct
     5281 gttggctggc tggtggcagg atatattgtg gtgtaaacaa attgacgctt agacaactta
     5341 ataacacatt gcggacgttt ttaatgtact gaattaacgc cgaattgaat tcgagctcgg
     5401 tacccgggga tcctctagag tcgacctgca ggcatgcaag cttagcttga gcttggatca
     5461 gattgtcgtt tcccgccttc agtttaaact atcagtgttt gacaggatat attggcgggt
     5521 aaacctaaga gaaaagagcg tttattagaa taatcggata tttaaaaggg cgtgaaaagg
     5581 tttatccgtt cgtccatttg tatgtgcatg ccaaccacag ggttcccctc gggatcaa
//
