LOCUS       40924_35813        7937 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pPZP200BAR, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7937)
  AUTHORS   Jozefkowicz C, Soto GC.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAY-2016) Instituto de Genetica 'Ewald A. Favret' 
            (IGEAF), National Institute of Agricultural Technology, Nicolas 
            Repetto y de los Reseros, Hurlingham, Buenos Aires 1686, Argentina
REFERENCE   2  (bases 1 to 7937)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7937)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (08-MAY-2016) Instituto de Genetica 'Ewald A. Favret' (IGEAF), 
            National Institute of Agricultural Technology, Nicolas Repetto y de 
            los Reseros, Hurlingham, Buenos Aires 1686, Argentina"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Sequencher v. 4.1.4
            Assembly Name         :: pPZP200BAR
            Coverage              :: 300x
            Sequencing Technology :: 454; Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7937
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(114..138)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    complement(195..251)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     promoter        334..513
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    562..617
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             619..1167
                     /codon_start=1
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      1189..1441
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    complement(1519..1543)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             2068..2856
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3103..3691
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(3877..4017)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4361..4555)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(4624..5688)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(6125..6751)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             complement(7050..7736)
                     /codon_start=1
                     /product="resolvase"
                     /label=resolvase
                     /protein_id="ANN46249.1"
                     /translation="MNKSAAAGLLGYARVSTDDQDLTNQRAELHAAGCTKLFSEKITGT
                     RRDRPELARMLDHLRPGDVVTVTRLDRLARSTRDLLDIAERIQEAGAGLRSLAEPWADT
                     TTPAGRMVLTVFAGIAEFERSLIIDRTRSGREAAKARGVKFGPRPTLTPAQIAHARELI
                     DQEGRTVKEAAALLGVHRSTLYRALERSEEVTPTEARRRGAFREDALTEADALAAAENE
                     RQEEQA"
ORIGIN
        1 ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc
       61 ttttcacgcc cttttaaata tccgttattc taataaacgc tcttttctct taggtttacc
      121 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      181 tccaagctca agctaagctt gcatgcctgc aggtcgactc tagaggatcc ccgggtaccg
      241 agctcgaatt gatcatgagc ggagaattaa gggagtcacg ttatgacccc cgccgatgac
      301 gcgggacaag ccgttttacg tttggaactg acagaaccgc aacgttgaag gagccactga
      361 gccgcgggtt tctggagttt aatgagctaa gcacatacgt cagaaaccat tattgcgcgt
      421 tcaaaagtcg cctaaggtca ctatcagcta gcaaatattt cttgtcaaaa atgctccact
      481 gacgttccat aaattcccct cggtatccaa ttagagtctc atattcactc tcaactcgag
      541 tataagagct ctatttttac aacaattacc aacaacaaca aacaacaaac aacattacaa
      601 ttactattta caattaccat gagcccagaa cgacgcccgg ccgacatccg ccgtgccacc
      661 gaggcggaca tgccggcggt ctgcaccatc gtcaaccact acatcgagac aagcacggtc
      721 aacttccgta ccgagccgca ggaaccgcag gagtggacgg acgacctcgt ccgtctgcgg
      781 gagcgctatc cctggctcgt cgccgaggtg gacggcgagg tcgccggcat cgcctacgcg
      841 ggcccctgga aggcacgcaa cgcctacgac tggacggccg agtcaaccgt gtacgtctcc
      901 ccccgccacc agcggacggg actgggctcc acgctctaca cccacctgct gaagtccctg
      961 gaggcacagg gcttcaagag cgtggtcgct gtcatcgggc tgcccaacga cccgagcgtg
     1021 cgcatgcacg aggcgctcgg atatgccccc cgcggcatgc tgcgggcggc cggcttcaag
     1081 cacgggaact ggcatgacgt gggtttctgg cagctggact tcagcctgcc ggtaccgccc
     1141 cgtccggtcc tgcccgtcac cgagatttga tgaccctaga gtcaagcaga tcgttcaaac
     1201 atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata
     1261 taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt
     1321 atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac
     1381 aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat
     1441 cgaccaattc aattcggcgt taattcagta cattaaaaac gtccgcaatg tgttattaag
     1501 ttgtctaagc gtcaatttgt ttacaccaca atatatcctg ccaccagcca gccaacagct
     1561 ccccgaccgg cagctcggca caaaatcacc actcgataca ggcagcccat cagtccggga
     1621 cggcgtcagc gggagagccg ttgtaaggcg gcagactttg ctcatgttac cgatgctatt
     1681 cggaagaacg gcaactaagc tgccgggttt gaaacacgga tgatctcgcg gagggtagca
     1741 tgttgattgt aacgatgaca gagcgttgct gcctgtgatc aattcgggca cgaacccagt
     1801 ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact
     1861 ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt
     1921 cttgttatga catgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg
     1981 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc
     2041 agtcgcccta aaacaaagtt aaacatcatg ggggaagcgg tgatcgccga agtatcgact
     2101 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta
     2161 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg
     2221 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg
     2281 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt
     2341 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa
     2401 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg
     2461 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag
     2521 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg
     2581 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc
     2641 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg
     2701 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat
     2761 cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac
     2821 tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctagctag aaattcgttc
     2881 aagccgacgc cgcttcgccg gcgttaactc aagcgattag atgcactaag cacataattg
     2941 ctcacagcca aactatcagg tcaagtctgc ttttattatt tttaagcgtg cataataagc
     3001 cctacacaaa ttgggagata tatcatgcat gaccaaaatc ccttaacgtg agttttcgtt
     3061 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     3121 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     3181 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     3241 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     3301 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     3361 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     3421 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     3481 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     3541 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     3601 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     3661 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     3721 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     3781 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     3841 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac
     3901 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
     3961 cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc
     4021 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     4081 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     4141 ccgaaacgcg cgaggcaggg tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc
     4201 ggcttgtccg cgccctggta gattgcctgg ccgtaggcca gccatttttg agcggccagc
     4261 ggccgcgata ggccgacgcg aagcggcggg gcgtagggag cgcagcgacc gaagggtagg
     4321 cgctttttgc agctcttcgg ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt
     4381 tttaagagtt ttaataagtt ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt
     4441 tatatcagtc acttacatgt gtgaccggtt cccaatgtac ggctttgggt tcccaatgta
     4501 cgggttccgg ttcccaatgt acggctttgg gttcccaatg tacgtgctat ccacaggaaa
     4561 gagacctttt cgaccttttt cccctgctag ggcaatttgc cctagcatct gctccgtaca
     4621 ttaggaaccg gcggatgctt cgccctcgat caggttgcgg tagcgcatga ctaggatcgg
     4681 gccagcctgc cccgcctcct ccttcaaatc gtactccggc aggtcatttg acccgatcag
     4741 cttgcgcacg gtgaaacaga acttcttgaa ctctccggcg ctgccactgc gttcgtagat
     4801 cgtcttgaac aaccatctgg cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag
     4861 aaaacggccg atgccgggat cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg
     4921 gttcttgcct tctgtgatct cgcggtacat ccaatcagct agctcgatct cgatgtactc
     4981 cggccgcccg gtttcgctct ttacgatctt gtagcggcta atcaaggctt caccctcgga
     5041 taccgtcacc aggcggccgt tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt
     5101 gtttaaccga atgcaggttt ctaccaggtc gtctttctgc tttccgccat cggctcgccg
     5161 gcagaacttg agtacgtccg caacgtgtgg acggaacacg cggccgggct tgtctccctt
     5221 cccttcccgg tatcggttca tggattcggt tagatgggaa accgccatca gtaccaggtc
     5281 gtaatcccac acactggcca tgccggccgg ccctgcggaa acctctacgt gcccgtctgg
     5341 aagctcgtag cggatcacct cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc
     5401 cacgtccatg atgctgcgac tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa
     5461 atctggttgc tcgtcgccct tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg
     5521 ttgccgggat tctttgcgga ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc
     5581 ttctgcctcg atgcgttgcc gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc
     5641 atcacccagc gccgcgccga tttgtaccgg gccggatggt ttgcgaccgt cacgccgatt
     5701 cctcgggctt gggggttcca gtgccattgc agggccggca gacaacccag ccgcttacgc
     5761 ctggccaacc gcccgttcct ccacacatgg ggcattccac ggcgtcggtg cctggttgtt
     5821 cttgattttc catgccgcct cctttagccg ctaaaattca tctactcatt tattcatttg
     5881 ctcatttact ctggtagctg cgcgatgtat tcagatagca gctcggtaat ggtcttgcct
     5941 tggcgtaccg cgtacatctt cagcttggtg tgatcctccg ccggcaactg aaagttgacc
     6001 cgcttcatgg ctggcgtgtc tgccaggctg gccaacgttg cagccttgct gctgcgtgcg
     6061 ctcggacggc cggcacttag cgtgtttgtg cttttgctca ttttctcttt acctcattaa
     6121 ctcaaatgag ttttgattta atttcagcgg ccagcgcctg gacctcgcgg gcagcgtcgc
     6181 cctcgggttc tgattcaaga acggttgtgc cggcggcggc agtgcctggg tagctcacgc
     6241 gctgcgtgat acgggactca agaatgggca gctcgtaccc ggccagcgcc tcggcaacct
     6301 caccgccgat gcgcgtgcct ttgatcgccc gcgacacgac aaaggccgct tgtagccttc
     6361 catccgtgac ctcaatgcgc tgcttaacca gctccaccag gtcggcggtg gcccatatgt
     6421 cgtaagggct tggctgcacc ggaatcagca cgaagtcggc tgccttgatc gcggacacag
     6481 ccaagtccgc cgcctggggc gctccgtcga tcactacgaa gtcgcgccgg ccgatggcct
     6541 tcacgtcgcg gtcaatcgtc gggcggtcga tgccgacaac ggttagcggt tgatcttccc
     6601 gcacggccgc ccaatcgcgg gcactgccct ggggatcgga atcgactaac agaacatcgg
     6661 ccccggcgag ttgcagggcg cgggctagat gggttgcgat ggtcgtcttg cctgacccgc
     6721 ctttctggtt aagtacagcg ataaccttca tgcgttcccc ttgcgtattt gtttatttac
     6781 tcatcgcatc atatacgcag cgaccgcatg acgcaagctg ttttactcaa atacacatca
     6841 cctttttaga cggcggcgct cggtttcttc agcggccaag ctggccggcc aggccgccag
     6901 cttggcatca gacaaaccgg ccaggatttc atgcagccgc acggttgaga cgtgcgcggg
     6961 cggctcgaac acgtacccgg ccgcgatcat ctccgcctcg atctcttcgg taatgaaaaa
     7021 cggttcgtcc tggccgtcct ggtgcggttt catgcttgtt cctcttggcg ttcattctcg
     7081 gcggccgcca gggcgtcggc ctcggtcaat gcgtcctcac ggaaggcacc gcgccgcctg
     7141 gcctcggtgg gcgtcacttc ctcgctgcgc tcaagtgcgc ggtacagggt cgagcgatgc
     7201 acgccaagca gtgcagccgc ctctttcacg gtgcggcctt cctggtcgat cagctcgcgg
     7261 gcgtgcgcga tctgtgccgg ggtgagggta gggcgggggc caaacttcac gcctcgggcc
     7321 ttggcggcct cgcgcccgct ccgggtgcgg tcgatgatta gggaacgctc gaactcggca
     7381 atgccggcga acacggtcaa caccatgcgg ccggccggcg tggtggtgtc ggcccacggc
     7441 tctgccaggc tacgcaggcc cgcgccggcc tcctggatgc gctcggcaat gtccagtagg
     7501 tcgcgggtgc tgcgggccag gcggtctagc ctggtcactg tcacaacgtc gccagggcgt
     7561 aggtggtcaa gcatcctggc cagctccggg cggtcgcgcc tggtgccggt gatcttctcg
     7621 gaaaacagct tggtgcagcc ggccgcgtgc agttcggccc gttggttggt caagtcctgg
     7681 tcgtcggtgc tgacgcgggc atagcccagc aggccagcgg cggcgctctt gttcatggcg
     7741 taatgtctcc ggttctagtc gcaagtattc tactttatgc gactaaaaca cgcgacaaga
     7801 aaacgccagg aaaagggcag ggcggcagcc tgtcgcgtaa cttaggactt gtgcgacatg
     7861 tcgttttcag aagacggctg cactgaacgt cagaagccga ctgcactata gcagcggagg
     7921 ggttggatca aagtact
//
