LOCUS       40924_35738        7925 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pPYPX88, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7925)
  AUTHORS   Xiong AS, Yao QH, Peng RH, Li X, Fan HQ, Guo MJ, Zhang SL.
  TITLE     Isolation, characterization, and molecular cloning of the cDNA 
            encoding a novel phytase from Aspergillus niger 113 and high 
            expression in Pichia pastoris
  JOURNAL   J. Biochem. Mol. Biol. 37 (3), 282-291 (2004)
  PUBMED    15469708
REFERENCE   2  (bases 1 to 7925)
  AUTHORS   Yao Q, Peng R, Xiong A.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2002) Shanghai Academy of Agricultural Sciences, 
            Agro-Biotechnology Research Center, 2901 Beidi Rd, Shanghai 201106, 
            China
REFERENCE   3  (bases 1 to 7925)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7925)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Biochem.
            Mol. Biol."; date: "2004"; volume: "37"; issue: "3"; pages: 
            "282-291"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-NOV-2002) Shanghai Academy of Agricultural Sciences, 
            Agro-Biotechnology Research Center, 2901 Beidi Rd, Shanghai 201106, 
            China"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     NCBI staff are still waiting for submitters to provide appropriate 
            coding region information.
FEATURES             Location/Qualifiers
     source          1..7925
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..936
                     /label=modified modified AOX1 promoter
                     /note="modified modified AOX1 promoter"
                     /regulatory_class="promoter"
     misc_feature    937..1212
                     /label=similar to modified alpha factor secretion signal
                     /note="similar to modified alpha factor secretion signal"
     terminator      1343..1589
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     misc_feature    complement(1994..4527)
                     /label=similar to histidnol dehydrogenase
                     /note="similar to histidnol dehydrogenase"
     misc_feature    4771..5527
                     /label=AOX1 3' fragment
                     /note="region downstream of Pichia pastoris AOX1 gene"
     misc_feature    5670..5810
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5996..6584)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6758..7615)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7616..7720)
                     /label=AmpR promoter
     promoter        7925
                     /label=AOX1 promoter
                     /note="inducible promoter, regulated by methanol"
ORIGIN
        1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag
       61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt
      121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc
      181 agcccagtta ttgggcttga ttggcgctca ttccaattcc ttctattagg ctactaacac
      241 catgacttta ttagcctgtc tatcctggcc cccctggcga ggttcatgtt tgtttatttc
      301 cgaatgcaac aagctccgca ttacacccga acatcactcc agatgagggc tttctgagtg
      361 tggggtcaaa tagtttcatg ttccccaaat ggcccaaaac tgacagttta aacgctgtct
      421 tggaacctaa tatgacaaaa gcgtgatctc atccaagatg aactaagttt ggttcgttga
      481 aatgctaacg gccagttggt caaaaagaaa cttccaaaag tcgccatacc gtttgtcttg
      541 tttggtattg attgacgaat gctcaaaaat aatctcatta atgcttagcg cagtctctct
      601 atcgcttctg aaccccggtg cacctgtgcc gaaacgcaaa tggggaaaca cccgcttttt
      661 ggatgattat gcattgtctc cacattgtat gcttccaaga ttctggtggg aatactgctg
      721 atagcctaac gttcatgatc aaaatttaac tgttctaacc cctacttgac agcaatatat
      781 aaacagaagg aagctgccct gtcttaaacc ttttttttta tcatcattat tagcttactt
      841 tcataattgc gactggttcc aattgacaag cttttgattt taacgacttt taacgacaac
      901 ttgagaagat caaaaaacaa ctaattattc gaaaccatgg ctattccacc atctatcttc
      961 atcgctgtct tgttcgctgc ttcttctgcc ttggctgctc ctgtcaacac tactaccgag
     1021 gatgaaactg ctcaaatccc tgctgaggct gtcatcggtt actctgacct ggagggtgac
     1081 ttcgacgtcg ctgtcttgcc attctctaac tccaccaaca acggtttgtt ggaggaggct
     1141 gaagctgaag ctgaacctaa attcatcaac actactatcg cttctatcgc tgctaaggag
     1201 gagggtgttt ccctcgagaa aagagaggct gaggcttacg tagagttgag ctcgggcggc
     1261 cgcgaattaa ttcgccttag acatgactgt tcctcagttc aagttgggca cttacgagaa
     1321 gaccggtctt gctagattct aatcaagagg atgtcagaat gccatttgcc tgagagatgc
     1381 aggcttcatt tttgatactt ttttatttgt aacctatata gtataggatt ttttttgtca
     1441 ttttgtttct tctcgtacga gcttgctcct gatcagccta tctcgcagct gatgaatatc
     1501 ttgtggtagg ggtttgggaa aatcattcga gtttgatgtt tttcttggta tttcccactc
     1561 ctcttcagag tacagaagat taagtgagaa gttcgtttgt gcaagctagc tttaatgcgg
     1621 tagtttatca cagttaaatt gctaacgcag tcaggcaccg tgtatgaaat ctaacaatgc
     1681 gctcatcgtc atcctcggca ccgtcaccct ggatgctgta ggcataggct tggttatgcc
     1741 ggtactgccg ggcctcttgc gggatatcgt ccattccgac agcatcgcca gtcactatgg
     1801 cgtgctgcta gcgctatatg cgttgatgca atttctatgc gcacccgttc tcggagcact
     1861 gtccgaccgc tttggccgcc gcccagtcct gctcgcttcg ctacttggag ccactatcga
     1921 ctacgcgatc atggcgacca cacccgtcct gtggatctat cgaatctaaa tgtaagttaa
     1981 aatctctaaa taattaaata agtcccagtt tctccatacg aaccttaaca gcattgcggt
     2041 gagcatctag accttcaaca gcagccagat ccatcactgc ttggccaata tgtttcagtc
     2101 cctcaggagt tacgtcttgt gaagtgatga acttctggaa ggttgcagtg ttaactccgc
     2161 tgtattgacg ggcatatccg tacgttggca aagtgtggtt ggtaccggag gagtaatctc
     2221 cacaactctc tggagagtag gcaccaacaa acacagatcc agcgtgttgt acttgatcaa
     2281 cataagaaga agcattctcg atttgcagga tcaagtgttc aggagcgtac tgattggaca
     2341 tttccaaagc ctgctcgtag gttgcaaccg atagggttgt agagtgtgca atacacttgc
     2401 gtacaatttc aacccttggc aactgcacag cttggttgtg aacagcatct tcaattctgg
     2461 caagctcctt gtctgtcata tcgacagcca acagaatcac ctgggaatca ataccatgtt
     2521 cagcttgagc agaaggtctg aggcaacgaa atctggatca gcgtatttat cagcaataac
     2581 tagaacttca gaaggcccag caggcatgtc aatactacac agggctgatg tgtcattttg
     2641 aaccatcatc ttggcagcag taacgaactg gtttcctgga ccaaatattt tgtcacactt
     2701 aggaacagtt tctgttccgt aagccatagc agctactgcc tgggcgcctc ctgctagcac
     2761 gatacactta gcaccaacct tgtgggcaac gtagatgact tctggggtaa gggtaccatc
     2821 cttcttaggt ggagatgcaa aaacaatttc tttgcaacca gcaactttgg caggaacacc
     2881 cagcatcagg gaagtggaag gcagaattgc ggttccacca ggaatataga ggccaacttt
     2941 ctcaataggt cttgcaaaac gagagcagac tacaccaggg caagtctcaa cttgcaacgt
     3001 ctccgttagt tgagcttcat ggaatttcct gacgttatct atagagagat caatggctct
     3061 cttaacgtta tctggcaatt gcataagttc ctctgggaaa ggagcttcta acacaggtgt
     3121 cttcaaagcg actccatcaa acttggcagt tagttctaaa agggctttgt caccattttg
     3181 acgaacattg tcgacaattg gtttgactaa ttccataatc tgttccgttt tctggatagg
     3241 acgacgaagg gcatcttcaa tttcttgtga ggaggcctta gaaacgtcaa ttttgcacaa
     3301 ttcaatacga ccttcagaag ggacttcttt aggtttggat tcttctttag gttgttcctt
     3361 ggtgtatcct ggcttggcat ctcctttcct tctagtgacc tttagggact tcatatccag
     3421 gtttctctcc acctcgtcca acgtcacacc gtacttggca catctaacta atgcaaaata
     3481 aaataagtca gcacattccc aggctatatc ttccttggat ttagcttctg caagttcatc
     3541 agcttcctcc ctaattttag cgttcaaaca aaacttcgtc gtcaaataac cgtttggtat
     3601 aagaaccttc tggagcattg ctcttacgat cccacaaggt gcttccatgg ctctaagacc
     3661 ctttgattgg ccaaaacagg aagtgcgttc caagtgacag aaaccaacac ctgtttgttc
     3721 aaccacaaat ttcaagcagt ctccatcaca atccaattcg atacccagca acttttgagt
     3781 tcgtccagat gtagcacctt tataccacaa accgtgacga cgagattggt agactccagt
     3841 ttgtgtcctt atagcctccg gaatagactt tttggacgag tacaccaggc ccaacgagta
     3901 attagaagag tcagccacca aagtagtgaa tagaccatcg gggcggtcag tagtcaaaga
     3961 cgccaacaaa atttcactga cagggaactt tttgacatct tcagaaagtt cgtattcagt
     4021 agtcaattgc cgagcatcaa taatggggat tataccagaa gcaacagtgg aagtcacatc
     4081 taccaacttt gcggtctcag aaaaagcata aacagttcta ctaccgccat tagtgaaact
     4141 tttcaaatcg cccagtggag aagaaaaagg cacagcgata ctagcattag cgggcaagga
     4201 tgcaacttta tcaaccaggg tcctatagat aaccctagcg cctgggatca tcctttggac
     4261 aactctttct gccaaatcta ggtccaaaat cacttcattg ataccattat tgtacaactt
     4321 gagcaagttg tcgatcagct cctcaaattg gtcctctgta acggatgact caacttgcac
     4381 attaacttga agctcagtcg attgagtgaa cttgatcagg ttgtgcagct ggtcagcagc
     4441 atagggaaac acggcttttc ctaccaaact caaggaatta tcaaactctg caacacttgc
     4501 gtatgcaggt agcaagggaa atgtcatact tgaagtcgga cagtgagtgt agtcttgaga
     4561 aattctgaag ccgtattttt attatcagtg agtcagtcat caggagatcc tctacgccgg
     4621 acgcatcgtg gccgatggtg cttgggtgaa caggtttatt cctggcatcc actaaatata
     4681 gcatgccgcg gattccttgc ggcggcggtg ctaacggcct caacctacgc gtggctgctt
     4741 cctaatgcag gagtcgcata agggagagcg tcgagtatct atgattggaa gtatgggaat
     4801 ggtgataccc gcattcttca gtgtcttgag gtctcctatc agattatgcc caactaaagc
     4861 aaccggagga ggagatttca tggtaaattt ctctgacttt tggtcatcag tagactcgaa
     4921 ctgtgagact atctcggtta tgacagcaga aatgtccttc ttggagacag taaatgaagt
     4981 cccaccaata aagaaatcct tgttatcagg aacaaacttc ttgtttcgaa ctttttcggt
     5041 gccttgaact ataaaatgta gagtggatat gtcgggtagg aatggagcgg gcaaatgctt
     5101 accttctgga ccttcaagag gtatgtaggg tttgtagata ctgatgccaa cttcagtgac
     5161 aacgttgcta tttcgttcaa accattccga atccagagaa atcaaagttg tttgtctact
     5221 attgatccaa gccagtgcgg tcttgaaact gacaatagtg tgctcgtgtt ttgaggtcat
     5281 ctttgtatga ataaatctag tctttgatct aaataatctt gacgagccaa ggcgataaat
     5341 acccaaatct aaaactcttt taaaacgtta aaaggacaag tatgtctgcc tgtattaaac
     5401 cccaaatcag ctcgtagtct gatcctcatc aacttgaggg gcactatctt gttttagaga
     5461 aatttgcgga gatgcgatat cgagaaaaag gtacgctgat tttaaacgtg aaatttatct
     5521 caagatctct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     5581 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     5641 gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc
     5701 ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     5761 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg
     5821 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     5881 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     5941 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     6001 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     6061 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     6121 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     6181 cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     6241 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     6301 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     6361 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     6421 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     6481 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     6541 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     6601 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     6661 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     6721 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     6781 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     6841 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     6901 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     6961 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     7021 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg
     7081 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     7141 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     7201 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     7261 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     7321 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata
     7381 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     7441 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     7501 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     7561 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     7621 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     7681 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     7741 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     7801 cgaggccctt tcgtcttcaa gaattaattc tcatgtttga cagcttatca tcgataagct
     7861 gactcatgtt ggtattgtga aatagacgca gatcgggaac actgaaaaat aacagttatt
     7921 attcg
//
