LOCUS       40924_35713        9482 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Inducible shuttle vector pPW578, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9482)
  AUTHORS   Woo PC, Ma SS, Teng JL, Li MW, Kao RY, Lau SK, Yuen KY.
  TITLE     Construction of an inducible expression shuttle vector for 
            Laribacter hongkongensis, a novel bacterium associated with 
            gastroenteritis
  JOURNAL   FEMS Microbiol. Lett. 252 (1), 57-65 (2005)
  PUBMED    16165318
REFERENCE   2  (bases 1 to 9482)
  AUTHORS   Woo PCY., Ma SSL., Teng JLL., Li MWS., Lau SKP., Yuen KY.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-AUG-2005) Microbiology, The University of Hong Kong, 
            Hong Kong
REFERENCE   3  (bases 1 to 9482)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9482)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS 
            Microbiol. Lett."; date: "2005"; volume: "252"; issue: "1"; pages: 
            "57-65"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-AUG-2005) Microbiology, The University of Hong Kong, Hong Kong"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9482
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      7..462
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    complement(469..590)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     952..968
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        975..993
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1981..2604
                     /codon_start=1
                     /gene="parA"
                     /product="ATPase"
                     /label=parA
                     /note="involved in chromosome partitioning"
                     /protein_id="AAZ91681.1"
                     /translation="MRTIVVASQKGGVGKTTIAGHLGVMAEQSKEGPVALIDTDPQGSL
                     ASWWNERTDEAPLFARVEINKLTEHLQALAKGGIKLAIIDTPPSVTEMIQQVLRTADLV
                     LIPTRPSPHDLRAVGSTVELVENAGKRMIFVINGAAPRARIAGEAAVALSQHGTVAPVT
                     LYQRTDFASSMIDGRTVQEIDPKGRSAEEIGKLWKYVSTQLRKI"
     gene            1981..2604
                     /gene="parA"
                     /label=parA
     CDS             3058..3975
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein"
                     /label=repA
                     /note="plasmid replication initiator protein"
                     /protein_id="AAZ91682.1"
                     /translation="MAEVVDEQPARRKRTPKGPRGLMPVHHPNRDFFLCDLFNYALKDD
                     GVSMEAPIFTLATKPDTSVWHWESKDGARAITVTPSVKGRATQFDKDLLIYVVSQMTEA
                     MNRGRPDANSRTVRFRVYDYLVSTNKPTGGKEYQRLEDALDRLRGTSIKTNIKTGGQRV
                     KEGFGIVDSWTIIEKAPDDDRMIAVEVTLSKWLFNAVQAHEVLTINPDYFRLRKPIERR
                     LYELARKHCGDQAFFVIGLELLQDKCGSKSALFEFRRALREIIKADTLPDYRMTLDNEK
                     DQVIFYTRDAKKLTASAALPQRFQ"
     gene            3058..3975
                     /gene="repA"
                     /label=repA
     misc_feature    5829..5866
                     /label=multiple cloning site
                     /note="multiple cloning site"
     protein_bind    5899..5917
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    complement(5984..6002)
                     /label=tet operator
                     /note="bacterial operator O1 for the tetR and tetA genes"
     CDS             6024..6647
                     /codon_start=1
                     /label=TetR
                     /note="tetracycline repressor TetR"
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     rep_origin      complement(6974..7562)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    complement(7891..7938)
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(8173..8964)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(8999..9356)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        complement(9358..9462)
                     /label=AmpR promoter
ORIGIN
        1 cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
       61 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
      121 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
      181 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
      241 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
      301 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
      361 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
      421 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttacgcgtta agatacattg
      481 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
      541 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
      601 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt
      661 aaaacctcta caaatgtggt atggctgatt atgatcatga acagactgtg aggactgagg
      721 ggcctgaaat gagccttggg actgtgaatc taaaatacac aaacaattag aatcagtagt
      781 ttaacacatt atacacttaa aaattggatc tccattcgcc attcaggctg cgcaactgtt
      841 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
      901 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
      961 cggccagtga attgtaatac gactcactat agggcgaatt gggtacactt acctggtacc
     1021 ccacccgggt ggaaaatcga tgggcccgcg gccgctctag aagtactctc gagaagcttt
     1081 ttgaattcac atcaaaataa ctattcatgt cgttttacat cgattcagat tttgaatgtt
     1141 atattcaata acgttacaat tgactccaac atcagcaata cattaactag gttaaaaatt
     1201 tacgctactg atctaaaaaa catgttggag catggagcac agtcagaaag catcaaagaa
     1261 aaggctatct caattaactc aacaatcgaa gtcttgatgg attcaattga agaggttgaa
     1321 gaaatatgca gaatcgactt ttcaaagaaa ttaattgagt cctgcaatgt aattattggt
     1381 acttatgata ccataaataa cgaggagggt tgggctccta aattaagaga tgcgcttgaa
     1441 tgtttatgcc gtgatttttt cgatagcact ctaggtcatc acttaaatag tcacttttgg
     1501 ttcaatctca agtattacga atggttggct aaagaagatt taatttatac agaccattta
     1561 tacggcaata aaaacaaaat aaggcatcat tccgatgaat tgcttgaaaa attttttaaa
     1621 gaaaaaagag aggaaataat aaaaaaaatg ttgggcggaa gaatagatcg ctatagcgaa
     1681 actattattt ttgataaccc tctaagtaat aatgaaacaa aagccatctt tgaaacatat
     1741 aaaatgttgc agaaattatc acaaaaactt atctaaattg agtttaaaca ttttcattcc
     1801 tcgatcccga tagggaatac cattcgagtt atcgcgggtg accatcaaga atttcactgc
     1861 cgtctacggc acggagccgc tggtatgcag ctcgccatgc cgaagaacca gccggcttta
     1921 tttaatacgc aaatacgtat ttgcgtgata agataaattc atataaatgt aggtgcgtaa
     1981 atgcggacta ttgttgtcgc gagtcaaaag ggtggtgttg gcaagacgac gattgcaggc
     2041 cacttgggtg tcatggccga gcaaagcaaa gaggggccgg tggcgttgat cgatacagac
     2101 ccgcaaggct cgctggcatc ctggtggaat gagcgaaccg atgaggcccc gctgtttgct
     2161 agggtggaga ttaacaaact gactgaacac cttcaggcct tggccaaggg tggcatcaag
     2221 ctggccatca tcgatacccc gccctcggtt acagaaatga ttcagcaagt gctccgcacc
     2281 gccgacttgg ttctaatccc tactaggccg agtccgcatg atttgcgagc ggtcggttct
     2341 accgtcgagc tggtggagaa cgcgggcaag cgaatgatct tcgtcatcaa tggagctgcg
     2401 cctcgcgcac ggatcgcagg tgaggctgcc gtggcgcttt cgcagcatgg caccgttgcc
     2461 cctgtgacgc tgtaccaacg caccgatttc gccagctcga tgatcgacgg ccgcacagtt
     2521 caggaaatcg atcccaaggg acggtctgcc gaagagatcg ggaaactgtg gaaatacgta
     2581 tctacacaac tgcgtaaaat ttgatatatt acgtaaatac gcaccaatgg agatacgtaa
     2641 atggctaaga ctgcatcttt gactgccgga ttagtggcca agaaaggtga ggcgtctccc
     2701 gctacggtcg tagcagccca accgacgcaa attatcgaag tgaaggtcgc agcgactggc
     2761 ggccgagatt actacaaggc attgaccgtc aagctggatc gtaaccgcta cgagagtctg
     2821 aaaagcatgg gagtgaagct ggacaaaaag agccaggaaa tctttgtcga agctctggac
     2881 ttgtggatga agtcggccgc tagccaacaa cacgcctaag aggcttccat gcgctctgct
     2941 gtcgaactcg ccaaagagtt ggctgaaaaa gccaaggccc gccgcctagc agcggaaaag
     3001 aatgagcttg aaggctcggc tcaggccgat gcaggcacgc ccggcccggt ggaggtcatg
     3061 gccgaagtgg tagacgagca accagcacgc cgcaagagaa cgccgaaagg gccgcgtggc
     3121 ctgatgccgg tacaccatcc aaaccgcgac ttctttttgt gtgatctgtt caactacgcc
     3181 ctgaaggatg acggcgtgag catggaggct cccatcttca ccttggcaac caagccagac
     3241 acctcagttt ggcattggga gagcaaagac ggggcacgcg caatcaccgt cacaccaagt
     3301 gtgaagggta gagctacgca gtttgataag gatttactta tctacgtagt tagtcagatg
     3361 accgaagcca tgaaccgagg tcggccggat gccaatagcc gcaccgtgcg ctttcgcgtc
     3421 tatgactacc tggtttcgac caacaagccg actggcggta aggaatacca acgcttggaa
     3481 gatgctctag acagactgcg gggtacatcg atcaagacga acatcaagac gggtggccag
     3541 cgtgtaaagg aaggcttcgg catagttgat agctggacga tcatcgagaa ggctcccgat
     3601 gatgatcgca tgattgccgt cgaggtcacg ctatccaagt ggcttttcaa tgcagtgcag
     3661 gcccacgagg ttctgaccat taacccggac tatttccggc tgcgtaagcc aatcgagcgc
     3721 cgtttgtacg aactagccag gaagcactgt ggcgaccagg ccttttttgt gattgggttg
     3781 gaactgctgc aggacaagtg cggcagcaag tcggcgctgt tcgagttccg ccgtgccttg
     3841 cgcgagatca tcaaggccga caccttgcca gactaccgca tgacgcttga taacgagaaa
     3901 gaccaagtga ttttctacac ccgcgacgcg aagaagctga cagcttccgc tgctctgcca
     3961 cagcgcttcc agtgactcca aagtattgac cgtcaataat ttcgttattt cacccatgca
     4021 atgctctagc tgcttgcttt gacctaacga gctgtcacgc tgcgcattga gccatcaatg
     4081 cgtcttactc cttcgagcac caagccatag cgcgtctcat gcgactttcg cgcgtgaaag
     4141 tcgaggaaag agacttgagc gttcttatcg ttacatcacc catgcctgtg gacagacacg
     4201 ttatatcacc catgttttct gtagcagaga cacgttacat cacccatgca ctacttcgtt
     4261 acatcaccca tgcagcagtg ttttataatc atttgaaaac atatgcttat gaccgatatc
     4321 cacagtcgta acacgcgcgc gcggttttta acttataaaa tctttaacgc gatggcgaca
     4381 aaaaccatcg ccatttcttg atgacaccat ccctatcgtc tctgacaaac cgttcagagt
     4441 gcagagcact cttgcccggc attccctgaa tttcgcttcg cagtaatcca taacctcgac
     4501 aaaccactca ggtcagtcac cttctcccgt ttcccacctc ttttccacga aaacctgctc
     4561 tccctccgca tcgagaacag gcacaccagt agcgcatgcc tgttagctgc gttttacggg
     4621 ccgtagcggg ccgtttacac ggctgctgat ggttagccac taaaacgaac aaaatcgcgc
     4681 ccaatagcct ttattgccca ttctccaacc atgagcgatc agtcatgcaa cggtagaaag
     4741 cagacgacaa tcacataccc cgcttgcgtg aaatccatcc taaatggacg aaacccgaaa
     4801 gggtctgaca tgcacaagaa gccatgctgg caggcgcaac ttgaatccct ctccgtgaag
     4861 gaacgaaaag cagatgaaag actgtgacca caacgaagcg atggcggatc tgtttcaggc
     4921 cgaaccaaga tttgctgcca cctatctgcg acaggtattg gctgatggcg agcctgccga
     4981 cgtacgcgcc ggcttgcggc aaatggcgga tgtcctgcgc gtcaaccagg ttgctgcact
     5041 gacagattcc acgccctcga cgggactctt tgaccgagcc ggcatgcgct acgaagtagc
     5101 atgcgacgtg atcggcgcat tgattgccca ttacaccgaa atcacgggac aggaacgtga
     5161 gcaggtacag ccggatgagg cggttttgcg cgtagccaag gctatgaagg cggcgctggc
     5221 cgaagagcgt gatgatctcg atccgagcaa tagcgtcggc atcgaggcag cgatttcgca
     5281 ctatgcgcca ctggcgcgcc ggctgtatgg cccggataaa aacgactccg tcagtcagaa
     5341 acagcgccgt gctggtttcg accaaggcaa tgtttcgcca gcccgggagg ggctggccat
     5401 gagcgcagat gatctggcgg tgcaggtgct gctgatccgg ggcaacatca cccacgatga
     5461 ggcagtgcag tgctaccgca tcctgcatcg ctatacacag taaaaacggc tggccagcca
     5521 aagtggccgg tgtgctggat ttacccgaat cggagccgcc aaaagcggca atgctgttgg
     5581 actgtttgat ggctgcggtg acccattccg ttcggacggc caagcggtca atgttcggca
     5641 aactgccgac agtagcccgg atggctgtcg gacggttttt cccgtttttg ctggcgggat
     5701 gcctggacga cgccggttac gaaaaaccgc atttgcccat gcaggcatgg ttcgttcgtc
     5761 agtggcgttt tcggtgaatg accgaattct ttggatccac tagtgtcgac ctgcaggcgc
     5821 gcgagctctt aattaacacg tgtccggaag atctaccggt gtcttcgagc tcggtaccat
     5881 caagcttatt ttaattatac tctatcaatg atagagtgtc aatatttttt ttagtttttc
     5941 atgaactcga ggggatccaa ataaaaaact agtttgacaa ataactctat caatgataga
     6001 gtgtcaacaa aaaggaggaa ttaatgatgt ctagattaga taaaagtaaa gtgattaaca
     6061 gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt aacaacccgt aaactcgccc
     6121 agaagctagg tgtagagcag cctacattgt attggcatgt aaaaaataag cgggctttgc
     6181 tcgacgcctt agccattgag atgttagata ggcaccatac tcacttttgc cctttagaag
     6241 gggaaagctg gcaagatttt ttacgtaata acgctaaaag ttttagatgt gctttactaa
     6301 gtcatcgcga tggagcaaaa gtacatttag gtacacggcc tacagaaaaa cagtatgaaa
     6361 ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt ttcactagag aatgcattat
     6421 atgcactcag cgctgtgggg cattttactt taggttgcgt attggaagat caagagcatc
     6481 aagtcgctaa agaagaaagg gaaacaccta ctactgatag tatgccgcca ttattacgac
     6541 aagctatcga attatttgat caccaaggtg cagagccagc cttcttattc ggccttgaat
     6601 tgatcatatg cggattagaa aaacaactta aatgtgaaag tgggtcttaa aagcagcata
     6661 acctttttcc gtgatggtaa cttcacggta accaagatgt cgagttatcg ataccgtcga
     6721 cctgcaggca tgcgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt
     6781 taccgtaagt tatgtaacgc ggaactccat atatgggcta tgaactaatg accccgtaat
     6841 tgattactat taataactaa tgcatggcgg taatacggtt atccacagaa tcaggggata
     6901 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     6961 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7021 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7081 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     7141 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     7201 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     7261 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     7321 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     7381 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     7441 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     7501 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     7561 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     7621 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     7681 aatgaagttt taaatcaatc taaagtatat atgagtaacc tgaggctatg gcagggcctg
     7741 ccgccccgac gttggctgcg agccctgggc cttcacccga acttgggggg tggggtgggg
     7801 aaaaggaaga aacgcgggcg tattggcccc aatggggtct cggtggggta tcgacagagt
     7861 gccagccctg ggaccgaacc ccgcgtttat gaacaaacga cccaacaccg tgcgttttat
     7921 tctgtctttt tattgccgtc atagcgcggg ttccttccgg tattgtctcc ttccgtgttt
     7981 cagttagcct ccccctaggg tgggcgaaga actccagcat gagatccccg cgctggagga
     8041 tcatccagcc ggcgtcccgg aaaacgattc cgaagcccaa cctttcatag aaggcggcgg
     8101 tggaatcgaa atctcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca
     8161 gagtcccgct cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg
     8221 agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc
     8281 aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca
     8341 gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc
     8401 atgggtcacg acgagatcct cgccgtcggg catgctcgcc ttgagcctgg cgaacagttc
     8461 ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc
     8521 catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc
     8581 cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg
     8641 agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct
     8701 tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca
     8761 cgatagccgc gctgcctcgt cttgcagttc attcagggca ccggacaggt cggtcttgac
     8821 aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat
     8881 tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc
     8941 gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat cctgtctctt gatcgatctt
     9001 tgcaaaagcc taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggccg
     9061 aggcggcctc ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg
     9121 cggaactggg cggagttagg ggcgggatgg gcggagttag gggcgggact atggttgctg
     9181 actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac
     9241 acctggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg
     9301 gggactttcc acaccctaac tgacacacat tccacagctg gttctttccg cctcaggact
     9361 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     9421 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     9481 gc
//
