LOCUS       40924_35683        6638 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPV529, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6638)
  AUTHORS   Shao H, Li X, Lok JB.
  TITLE     Heritable genetic transformation of Strongyloides stercoralis by 
            microinjection of plasmid DNA constructs into the male germline
  JOURNAL   Int. J. Parasitol. (2017) In press
  PUBMED    28577882
REFERENCE   2  (bases 1 to 6638)
  AUTHORS   Shao H, Li X, Lok JB.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAR-2017) Pathobiology, University of Pennsylvania, 
            3800 Spruce Street, Rosenthal 212, Philadelphia, PA 19104, USA
REFERENCE   3  (bases 1 to 6638)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6638)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Int. J. 
            Parasitol. (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-MAR-2017) Pathobiology, University of Pennsylvania, 3800 Spruce 
            Street, Rosenthal 212, Philadelphia, PA 19104, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6638
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..131
                     /label=AmpR promoter
     CDS             132..989
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1163..1751
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2039..2060
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2075..2105
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2113..2129
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2137..2153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     mobile_element  2644..2678
                     /mobile_element_type="transposon:PiggyBac ITR"
                     /label=ITR
     regulatory      2954..4116
                     /regulatory_class="promoter"
     CDS             join(4129..4299,4351..4504,4556..4719,4771..4998)
                     /codon_start=1
                     /gene="gfp"
                     /product="green fluorescent protein"
                     /label=gfp
                     /note="GFP"
                     /protein_id="ARW80044.1"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     gene            4129..4998
                     /gene="gfp"
                     /label=gfp
     3'UTR           5018..5604
                     /gene="Ss era-1"
     gene            5018..5604
                     /gene="Ss era-1"
                     /label=Ss era-1
     primer_bind     5618..5634
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     mobile_element  5831..5893
                     /mobile_element_type="transposon:PiggyBac ITR"
                     /label=ITR
     primer_bind     complement(6023..6039)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      6181..6636
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 ttcgtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcccgccgg gtaactcacg gggtatccat gtccatttct gcggcatcca
     2221 gccaggatac ccgtcctcgc tgacgtaata tcccagcgcc gcaccgctgt cattaatctg
     2281 cacaccggca cggcagttcc ggctgtcgcc ggtattgttc gggttgctga tgcgcttcgg
     2341 gctgaccatc cggaactgtg tccggaaaag ccgcgacgaa ctggtatccc aggtggcctg
     2401 aacgaacagt tcaccgttaa aggcgtgcat ggccacacct tcccgaatca tcatggtaaa
     2461 cgtgcgtttt cgctcaacgt caatgcagca gcagtcatcc tcggcaaact ctttccatgc
     2521 cgcttcaacc tcgcgggaaa aggcacgggc ttcttcctcc ccgatgcctg gatagcgcca
     2581 gcttgggcga tgactgagcc ggaaaaaaga cccgacgata tgatcctgat gcagctagat
     2641 taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat attgctctct
     2701 ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg ccgcttggag
     2761 ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact ataacgaccg
     2821 cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta actcatacga
     2881 taattatatt gttatttcat gttctactta cgtgataact tattatatat atattttctt
     2941 gttatagata tcaagcttac catgatatta aataagtgaa agttttagtg gacttttgat
     3001 gaatgtgatg tttttgttaa agattttctt ttaaatacta atttttcaat ttttaaaaac
     3061 ttgcttttta ggtaataaga ttgtttaagg ttaaacgagg cattaaatca aatactagac
     3121 attttataac atctaccgat ttatcgtctt aagtcatatc ctcatttatc tcattaacat
     3181 atccagatac taatgttata tataaattat cattgttatt atatatatat ttattgtact
     3241 tttattgtag ttttcacttt ttaataaaaa tataaattga ttccagaatt gattttctaa
     3301 ttacaattta cttttgatac atcaaatact attctttatg acatctagtt ttaaaggata
     3361 tttatgtaat acaattattg cttgattgct ttattgtaat taaattatag tccatttaaa
     3421 aaaaaaaaat tttttttata tatatattca attaattaaa aaaatgttcc aaatgaattt
     3481 atattattac aagatataaa atggtataga aattttgtta ttaaaaattt taaatttcat
     3541 tttataaaca aaaagtcttt gaaaacaaat aaaactaaca caatactttt atttaatcaa
     3601 atacaataga tctttatatt tgtgctttta agttattaac aaatatttca attttcataa
     3661 aatacttcat ttttgaattt atgtaaattt ttttaaaagg catactttta gcgattgata
     3721 gttaacatat tcacgtcagt gcgacctcaa attttttcca ctagtactta tctataatga
     3781 taaaaaaaaa agaggggtgt ttaccgaaaa gttttaatag ccaatatacg tgatgtgcag
     3841 gcttttttac gtttttatta ccgtaaaaag tatagcgttt tggagattta tttataatta
     3901 gtatacataa tgccgatgct cagttatgtt tcttaaccag aataacctga atgtatagac
     3961 atttttccac aacccaaatg ttatattaac atttatattt aattgttata tattttcatc
     4021 ccacgttgca ttttagattt aatttttata tatttgaaaa tatattttaa tataattatt
     4081 tttatttaat tatagattca ttagctattt accataccgg tagaaaaaat gagtaaagga
     4141 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg
     4201 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt
     4261 aaatttattt gcactactgg aaaactacct gttccatggg taagtttaaa catatatata
     4321 ctaactaacc ctgattattt aaattttcag ccaacacttg tcactacttt ctgttatggt
     4381 gttcaatgct tctcgagata cccagatcat atgaaacggc atgacttttt caagagtgcc
     4441 atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag
     4501 acacgtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt ttcaggtgct
     4561 gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt
     4621 aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc acacaatgta
     4681 tacatcatgg cagacaaaca aaagaatgga atcaaagttg taagtttaaa catgatttta
     4741 ctaactaact aatctgattt aaattttcag aacttcaaaa ttagacacaa cattgaagat
     4801 ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc
     4861 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa
     4921 aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg
     4981 gatgaactat acaaatagca ttcgtagaat tcctaggaat ttattttgat aagtctcgcg
     5041 tattttattt gtaataatct ttttataaga tcaatttaca tttatactga taaaattgac
     5101 acaattacac tttttattat aaaatatttt aaaatatttc tatcaaaagg tttatttttg
     5161 ttttaaatat aatggttttt tttgtaaaaa atacttattt cgtttgataa tctttattca
     5221 gagatttgaa taattttaaa taaagtagtt tagttttcat ataaaagtta atggtatata
     5281 taaatattta aatagtaaag attgaatcag tattcaaaat gacacctaaa agttattcta
     5341 tttctagcta taacaataat aataaagata attatgatac agatttagaa aaaaaaataa
     5401 ggttaaatta attaattcta tttttaatta aaatgattta agggagttaa atgaaacaga
     5461 aacagattgg gtctctatat atttagttta tgtaatgtta ttttttggtg gaatacaaat
     5521 aacggtatat tttacttcaa tgtggccata cttatcaaca ataaatagta aggcaaatgt
     5581 agatacttta gggtgggttg tagcactagt cggccgctct agaactagtg gatcccccgg
     5641 gctgcaggaa ttcgataaaa gttttgttac tttatagaag aaattttgag tttttgtttt
     5701 tttttaataa ataaataaac ataaataaat tgtttgttga atttattatt agtatgtaag
     5761 tgtaaatata ataaaactta atatctattc aaattaataa ataaacctcg atatacagac
     5821 cgataaaaca catgcgtcaa ttttacgcat gattatcttt aacgtacgtc acaatatgat
     5881 tatctttcta gggttaatct agctgcgtgt tctgcagcgt gtcgagcatc ttcatctgct
     5941 ccatcacgct gtaaaacaca tttgcaccgc gagtctgccc gtcctccacg ggttcaaaaa
     6001 cgtgaatgaa cgaggcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     6061 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     6121 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     6181 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     6241 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     6301 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     6361 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     6421 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     6481 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     6541 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     6601 acgcgaattt taacaaaata ttaacgctta caatttag
//
