LOCUS       40924_35673        7535 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPV505, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7535)
  AUTHORS   Lok JB, Shao H, Massey HC, Li X.
  TITLE     Transgenesis in Strongyloides and related parasitic nematodes: 
            historical perspectives, current functional genomic applications and
            progress towards gene disruption and editing
  JOURNAL   Parasitology (2016) In press
  PUBMED    27000743
REFERENCE   2  (bases 1 to 7535)
  AUTHORS   Lok JB, Shao H, Massey HC Jr., Li X.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2016) Pathobiology, University of Pennsylvania, 
            3800 Spruce Street, Philadelphia, PA 19104, USA
REFERENCE   3  (bases 1 to 7535)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7535)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Parasitology (2016) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2016) Pathobiology, University of Pennsylvania, 3800 Spruce 
            Street, Philadelphia, PA 19104, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7535
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /label=AmpR promoter
     CDS             129..986
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1160..1748
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2072..2102
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(3315..3326)
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     misc_feature    4259..5124
                     /label=similar to green fluorescent protein
                     /note="similar to green fluorescent protein"
     misc_feature    5125..5227
                     /note="P2A; linker"
     CDS             5159..5215
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5228..5902
                     /label=mRFP1
                     /note="monomeric derivative of DsRed (Campbell et al.,
                     2002)"
     misc_feature    5939..6501
                     /note="Era1; UTR"
     primer_bind     6515..6531
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6920..6936)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      7078..7533
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc ccgccgggta actcacgggg tatccatgtc catttctgcg gcatccagcc
     2221 aggatacccg tcctcgctga cgtaatatcc cagcgccgca ccgctgtcat taatctgcac
     2281 accggcacgg cagttccggc tgtcgccggt attgttcggg ttgctgatgc gcttcgggct
     2341 gaccatccgg aactgtgtcc ggaaaagccg cgacgaactg gtatcccagg tggcctgaac
     2401 gaacagttca ccgttaaagg cgtgcatggc cacaccttcc cgaatcatca tggtaaacgt
     2461 gcgttttcgc tcaacgtcaa tgcagcagca gtcatcctcg gcaaactctt tccatgccgc
     2521 ttcaacctcg cgggaaaagg cacgggcttc ttcctccccg atgcctggat agcgccagct
     2581 tgggcgatga ctgagccgga aaaaagaccc gacgatatga tcctgatgca gctagattaa
     2641 ccctagaaag atagtctgcg taaaattgac gcatgcattc ttgaaatatt gctctctctt
     2701 tctaaatagc gcgaatccgt cgctgtgcat ttaggacatc tcagtcgccg cttggagctc
     2761 ccgtgaggcg tgcttgtcaa tgcggtaagt gtcactgatt ttgaactata acgaccgcgt
     2821 gagtcaaaat gacgcatgat tatcttttac gtgactttta agatttaact catacgataa
     2881 ttatattgtt atttcatgtt ctacttacgt gataacttat tatatatata ttttcttgtt
     2941 atagatatca agcttgatta tcgtagtgag aggacgtgta gagataataa atttcccaac
     3001 ttaatctatt ataggaactt agttaaaaat gattgtattg gtcctctttg tgaaagtgtg
     3061 gaaaagaggt acctcgtagt ttttttcttc tcaattctca tcatcaccct gtgtaacatc
     3121 acatctcata tagagaaatc gcgactttta ttgcttagca tatactttgt aagttgtttt
     3181 cactgaggct ttgtccatca cacgctacac attatcgctg ttatttttat tatttatatt
     3241 tattagtaat aatattaaat aaacacatag gaattttgtc tttgttggcc cttccagcct
     3301 aaatagttat aaaatctacc ttctattata catacgcata tgttatttaa aaagttgaag
     3361 tgatttctct tctacagaaa ctacacattt atcttcttat tttcttccaa acatatagca
     3421 ataataattt aaaacttttt cttactcctc ccagagtttg ttaagttgat catatttaaa
     3481 attcttctgt cttctacaac aaataatata tttattataa caccaactac ctcgttccaa
     3541 gtattacgta ggagcatata cataccacaa tgcttaggaa atgggggcgg ttatgatata
     3601 acattatttc aatgtaacat tcttttttac cacatatatt ttctactgaa ttctgaaaac
     3661 tcaatttcaa acctcctata tatatatatt gttaggcact tctaagatta taattcattt
     3721 aactctctta aatttatcta ttttaaattt ttcctttttt gtattattta tactattata
     3781 tcgaaacttt attattacat gtactattta acttttattt tcttatataa taataatact
     3841 aaataataac tgataatcgt atctgtagag attattgaat tgtgattagt aatgtaaagt
     3901 aaatctatat taagatatct caaagatgca ctcaagttat ttgaaaaatt tcttatacat
     3961 agattaatac ttatattaag aaactttaaa aaaaagtttt aaataaaaaa aaaaattcac
     4021 aattgtacta ttttatcttg tcgttcagtt tatcaccgta gtatttttat ggctagtaat
     4081 attcatcatt tttagataaa ctaacttgct atagaaaatg tgtgacgaag aggttgccgc
     4141 tgcaggtcga ctagaggatc cccgggattg gccaaaggac ccaaaggtat gtttcgaatg
     4201 atactaacat aacatagaac attttcagga ggacccttgg agggtaccgg tagaaaaaat
     4261 gagtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga
     4321 tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa
     4381 acttaccctt aaatttattt gcactactgg aaaactacct gttccatggg taagtttaaa
     4441 catatatata ctaactaacc ctgattattt aaattttcag ccaacacttg tcactacttt
     4501 ctgttatggt gttcaatgct tctcgagata cccagatcat atgaaacggc atgacttttt
     4561 caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg
     4621 gaactacaag acacgtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt
     4681 ttcaggtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg
     4741 tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc
     4801 acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagttg taagtttaaa
     4861 catgatttta ctaactaact aatctgattt aaattttcag aacttcaaaa ttagacacaa
     4921 cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga
     4981 tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga
     5041 tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac
     5101 acatggcatg gatgaactat acaatccgga attcgtagaa ttgctagccg gaagcggagc
     5161 tactaacttc agcctgctga agcaggctgg agacgtggag gagaaccctg gacctcggcc
     5221 ggtaaaaatg gcatcatcag aagatgttat taaagaattt atgagattca aagttaaaat
     5281 ggaaggttca gttaacggtc atgaatttga aattgaaggt gaaggtgaag gtagaccata
     5341 tgaaggtaca caaacagcaa aattaaaagt tacaaaaggt ggtccattac catttgcatg
     5401 ggatattctt tcaccacaat ttcaatatgg tagtaaagca tatgttaaac atccagcaga
     5461 tattccagat tatcttaaac tttcatttcc agaaggtttt aaatgggaaa gaattatgaa
     5521 ttttgaagat ggtggtgttg taacagttac tcaagattca acattacaag atggtgaatt
     5581 tatttataaa gttaaattaa gaggtaccaa ttttccatca gatggtccag taatgcaaaa
     5641 aaaaactatg ggttgggaag catcaacaga aagaatgtat ccagaagatg gtgcactaaa
     5701 aggtgaaatt aaaatgagat taaaacttaa ggatggtggt cattatgatg cagaagttaa
     5761 aacaacttat aaagctaaaa aacaagttca attaccaggt gcttataaaa cagatattaa
     5821 attagatatt atttcacata atgaagatta tactattgtt gaacaatatg aaagagcaga
     5881 aggtagacat tcaacaggtg caggatctcc taggaattta ttttgataag tctcgcgtat
     5941 tttatttgta ataatctttt tataagatca atttacattt atactgataa aattgacaca
     6001 attacacttt ttattataaa atattttaaa atatttctat caaaaggttt atttttgttt
     6061 taaatataat ggtttttttt gtaaaaaata cttatttcgt ttgataatct ttattcagag
     6121 atttgaataa ttttaaataa agtagtttag ttttcatata aaagttaatg gtatatataa
     6181 atatttaaat agtaaagatt gaatcagtat tcaaaatgac acctaaaagt tattctattt
     6241 ctagctataa caataataat aaagataatt atgatacaga tttagaaaaa aaaataaggt
     6301 taaattaatt aattctattt ttaattaaaa tgatttaagg gagttaaatg aaacagaaac
     6361 agattgggtc tctatatatt tagtttatgt aatgttattt tttggtggaa tacaaataac
     6421 ggtatatttt acttcaatgt ggccatactt atcaacaata aatagtaagg caaatgtaga
     6481 tactttaggg tgggttgtag cactagtcgg ccgctctaga actagtggat cccccgggct
     6541 gcaggaattc gataaaagtt ttgttacttt atagaagaaa ttttgagttt ttgttttttt
     6601 ttaataaata aataaacata aataaattgt ttgttgaatt tattattagt atgtaagtgt
     6661 aaatataata aaacttaata tctattcaaa ttaataaata aacctcgata tacagaccga
     6721 taaaacacat gcgtcaattt tacgcatgat tatctttaac gtacgtcaca atatgattat
     6781 ctttctaggg ttaatctagc tgcgtgttct gcagcgtgtc gagcatcttc atctgctcca
     6841 tcacgctgta aaacacattt gcaccgcgag tctgcccgtc ctccacgggt tcaaaaacgt
     6901 gaatgaacga ggcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     6961 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     7021 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg
     7081 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     7141 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     7201 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     7261 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     7321 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     7381 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     7441 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     7501 cgaattttaa caaaatatta acgcttacaa tttag
//
