LOCUS       40924_35653        7139 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression indicator vector pPV238, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7139)
  AUTHORS   Massey HC Jr., Ranjit N, Stoltzfus JD, Lok JB.
  TITLE     Strongyloides stercoralis daf-2 encodes a divergent ortholog of 
            Caenorhabditis elegans DAF-2
  JOURNAL   Int. J. Parasitol. 43 (7), 515-520 (2013)
  PUBMED    23500073
REFERENCE   2  (bases 1 to 7139)
  AUTHORS   Massey HC Jr., Ranjit N, Stoltzfus JD, Castelletto ML, Lok JB.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2012) Pathobiology, University of Pennsylvania 
            Veterinary School, 3800 Spruce Street, Philadelphia, PA 19104, USA
REFERENCE   3  (bases 1 to 7139)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7139)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Int. J. 
            Parasitol."; date: "2013"; volume: "43"; issue: "7"; pages: 
            "515-520"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-JUN-2012) Pathobiology, University of Pennsylvania Veterinary 
            School, 3800 Spruce Street, Philadelphia, PA 19104, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7139
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1401..1415
                     /codon_start=1
                     /label=enterokinase site
                     /note="enterokinase recognition and cleavage site"
                     /translation="DDDDK"
     CDS             join(2779..2949,3001..3154,3206..3369,3421..3648)
                     /codon_start=1
                     /product="GFP S65C"
                     /label=GFP S65C
                     /note="Aequorea victoria green fluorescent protein"
                     /protein_id="AFQ32906.1"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     3'UTR           3785..4519
                     /label=Caenorhabditis elegans unc-54 3'UTR
                     /note="Caenorhabditis elegans unc-54 3'UTR"
     promoter        4884..4988
                     /label=AmpR promoter
     CDS             4989..5846
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6020..6608
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    6896..6917
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6932..6962
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6970..6986
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6994..7010
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagcttctgc agttgttgct atctctatct cttggctccg agagctctgc tctagaattg
       61 tccctactca ttctttgatc taagacgacg agacatttgc agaaagtgct tgtaaagatg
      121 ctgctgctgc ttgaataaac tccaaaatag tattcaaatt ctgaaaaacc aaaaaaattt
      181 caattttggc taaattcaaa ctgcaaccaa tcagcgattc gccccgccca cttttgaacc
      241 aatcaggctg aacgagtccg cccactcctt ctgattggtt cgaaagtggg tggagagaaa
      301 tggggattgg ttgcagttcg aatctagcca aaattggaaa cattgcattt taaattgttg
      361 gatttttcaa ccttatccgg tttcccggca aatcggcaat tttctgtttt gccggaaatt
      421 tcaaattcct gcaaattgcc gttttgccga tttgccggaa attttcaatt ctggcagttt
      481 gccgattttt cgggaaattt aaattctggc tttggatgtt tggttgcagt tcgaatttag
      541 tttttaaatt tcaaattccg gcaattttct gatttgccgg aagttttaaa tgtccggcaa
      601 tttgccgttt tgccggaaat tttaaattcc ggcaatttgc caattttcct gaaattttaa
      661 attccggcaa tttgtcaatt tcctgaaatt ttaaaatccg gcaatttgcc aattttcctg
      721 aaattttaaa ttccggcaat ttgccgtttt gccggtaatt ttcaattccg gcaatttgcc
      781 gttttgccgg aaattttctg atttgccggt tttctggcaa aatgacaatt ttctgatttg
      841 caggaaaatt taaattccgg caattttcca atttgccgga agttttaaat tccggcaatt
      901 tgccaatttt cctgaaattt taaattccgg cacttttcca atttgccgga aattttaaaa
      961 tccggcaatt tgccaatttt cctgaaattt taaattccgg caatttgccg ttttgccggt
     1021 aattttcaat tccggcaatt ttccaattcg ccggaaagtt tcaattccgg caattttcca
     1081 atttgccgga aagtttcaat tccggcaatt cgccattaac acgtggtgtc agagaacgtt
     1141 ctgacgtcac atttttctga gcgaaaaatt cccacatttt ttgtagatca aaccgtaatg
     1201 ggatagcctg gtgcatttag agcattctaa accacaattc ccaataaata atagttgcaa
     1261 aagtaatcta tttttctcga aaacacgaaa tattgtagcc tctcatttct ttttcttatc
     1321 aaaactatca tcatcatcat catctttttc ttcgttatta ccgagaaaag cgggaggaaa
     1381 tgcgaaaaga aatagaagaa gacgatgatg ataaagatga agaaaaataa taaaaagata
     1441 acaaaaaatc acatgataca caaaaaaaat gtacagtgtg tgtgtgtgta cataataaat
     1501 aacgactaat tgtatcaccc ctaatcggag aaatcaaaaa aaaaaaaatt aaacaatttt
     1561 tcgattattt ccgtattaac tgtttataat ttgggtcact ctgtgttcct tttgatctcg
     1621 aatttttttt ttctgtaaaa cagttttttg gccgaggaaa cgccggtcgg tggcctaggt
     1681 tttcgattcg cggccacgga atttgagaaa aaagctgaaa tttcggaaaa aaaatctaaa
     1741 aattcaaaaa aaaaaagttt tggaggaaat ttgaaaaata actttaagaa attcagaaaa
     1801 accacaaaat tcagaaaaaa aaattaaaaa ttcggagttt ttcagaaaaa aaaagtaaaa
     1861 acattgaaca aataaaaaaa acaaaaaaaa ctgtttaaaa aatttgaaaa ttcaaaaaaa
     1921 aaaaccgaaa aaaattcaaa agtcttaaaa cacctgaaaa tctgattttt tctgaaattt
     1981 cagacaaatt tcaaaaattt agctaaaaat ctgaatattc ccataagaaa actcataatt
     2041 caaaaaaaat ctaaacttaa ttattttttt tttgaaaatt cgaaaaaaaa accattaaaa
     2101 atgtgtaaaa agtcaaattt cagtttaaaa aaaatctgac aatttttaaa aaccaaaaaa
     2161 actaaatttc taaacaaatt tttagagatt caaatattca gagaaaatta gatttttttt
     2221 cgaaatttca aacaaatttg aaaattcaaa aaaaaatcta aactttaaaa aattgaaatt
     2281 acaggaaaaa tctgaaaatt aactaaaaat ttgatttttt tttagaaaaa aaatgtgtag
     2341 aaaagtccaa attccagaaa aagtccagtt tttttcctga attttcagct ttttccaaaa
     2401 aaaaaaaaat tactatttta taattttttt tggaaaaaat ctgaaaattc tgaacttact
     2461 tccccaattt cttcctctct cgaacacatt ttccaacaaa ttataatttc cgaaaattgt
     2521 catttttttt cctcaaaatg tagtaaacaa catttcttct ccacccttat cacagttttt
     2581 ttctctcttc ttcatctccc tccatcacca aaaaaatttc ttaaaacatc atcatcatga
     2641 attgcattca atttgcagct ccaagagcac acatctgatc gtcggattct actgtactcc
     2701 ccgaaaaacc aacaaaaaac acaagttttt gaacacttgt aaatgcagac agaacgatga
     2761 cgagaaccgg tagaaaaaat gagtaaagga gaagaacttt tcactggagt tgtcccaatt
     2821 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa
     2881 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct
     2941 gttccatggg taagtttaaa catatatata ctaactaacc ctgattattt aaattttcag
     3001 ccaacacttg tcactacttt ctgttatggt gttcaatgct tctcgagata cccagatcat
     3061 atgaaacggc atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact
     3121 atatttttca aagatgacgg gaactacaag acacgtaagt ttaaacagtt cggtactaac
     3181 taaccataca tatttaaatt ttcaggtgct gaagtcaagt ttgaaggtga tacccttgtt
     3241 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa
     3301 ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga
     3361 atcaaagttg taagtttaaa catgatttta ctaactaact aatctgattt aaattttcag
     3421 aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa
     3481 caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca
     3541 caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt
     3601 gtaacagctg ctgggattac acatggcatg gatgaactat acaaatagca ttcgtagaat
     3661 tccaactgag cgccggtcgc taccattacc aacttgtctg gtgtcaaaaa taataggggc
     3721 cgctgtcatc agagtaagtt taaactgagt tctactaact aacgagtaat atttaaattt
     3781 tcagcatctc gcgcccgtgc ctctgacttc taagtccaat tactcttcaa catccctaca
     3841 tgctctttct ccctgtgctc ccacccccta tttttgttat tatcaaaaaa acttcttctt
     3901 aatttctttg ttttttagct tcttttaagt cacctctaac aatgaaattg tgtagattca
     3961 aaaatagaat taattcgtaa taaaaagtcg aaaaaaattg tgctccctcc ccccattaat
     4021 aataattcta tcccaaaatc tacacaatgt tctgtgtaca cttcttatgt tttttttact
     4081 tctgataaat tttttttgaa acatcataga aaaaaccgca cacaaaatac cttatcatat
     4141 gttacgtttc agtttatgac cgcaattttt atttcttcgc acgtctgggc ctctcatgac
     4201 gtcaaatcat gctcatcgtg aaaaagtttt ggagtatttt tggaattttt caatcaagtg
     4261 aaagtttatg aaattaattt tcctgctttt gctttttggg ggtttcccct attgtttgtc
     4321 aagagtttcg aggacggcgt ttttcttgct aaaatcacaa gtattgatga gcacgatgca
     4381 agaaagatcg gaagaaggtt tgggtttgag gctcagtgga aggtgagtag aagttgataa
     4441 tttgaaagtg gagtagtgtc tatggggttt ttgccttaaa tgacagaata cattcccaat
     4501 ataccaaaca taactgtttc ctactagtcg gccgtacggg ccctttcgtc tcgcgcgttt
     4561 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
     4621 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     4681 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     4741 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcggc cttaagggcc
     4801 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     4861 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     4921 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     4981 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     5041 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     5101 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     5161 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     5221 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     5281 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     5341 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     5401 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     5461 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     5521 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     5581 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     5641 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     5701 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     5761 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     5821 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     5881 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     5941 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     6001 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     6061 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     6121 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     6181 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     6241 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     6301 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     6361 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag cattgagaaa
     6421 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     6481 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     6541 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     6601 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     6661 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     6721 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     6781 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     6841 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     6901 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     6961 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     7021 ccaagctgta agtttaaaca tgatcttact aactaactat tctcatttaa attttcagag
     7081 cttaaaaatg gctgaaatca ctcacaacga tggatacgct aacaacttgg aaatgaaat
//
