LOCUS       V003909                 6218 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003909
VERSION     V003909
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6218)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6218)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6218)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6218)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6218
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             639..2309
                     /gene="PTBP1"
                     /label="Polypyrimidine tract-binding protein 1"
                     /note="Polypyrimidine tract-binding protein 1 from Homo
                     sapiens. Accession#: P26599"
     CDS             2316..2999
                     /label="HcRed1"
                     /note="far-red fluorescent variant of Heteractis crispa
                     chromoprotein"
     polyA_signal    3006..3127
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3134..3589)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3616..3720
                     /label="AmpR promoter"
     promoter        3722..4079
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4114..4905
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5140..5187
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5516..6104
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attccaccat ggacggcatt gtcccagata
      661 tagccgttgg tacaaagcgg ggatctgacg agcttttctc tacttgtgtc actaacggac
      721 cgtttatcat gagcagcaac tcggcttctg cagcaaacgg aaatgacagc aagaagttca
      781 aaggtgacag ccgaagtgca ggcgtcccct ctagagtgat ccacatccgg aagctcccca
      841 tcgacgtcac ggagggggaa gtcatctccc tggggctgcc ctttgggaag gtcaccaacc
      901 tcctgatgct gaaggggaaa aaccaggcct tcatcgagat gaacacggag gaggctgcca
      961 acaccatggt gaactactac acctcggtga cccctgtgct gcgcggccag cccatctaca
     1021 tccagttctc caaccacaag gagctgaaga ccgacagctc tcccaaccag gcgcgggccc
     1081 aggcggccct gcaggcggtg aactcggtcc agtcggggaa cctggccttg gctgcctcgg
     1141 cggcggccgt ggacgcaggg atggcgatgg ccgggcagag ccccgtgctc aggatcatcg
     1201 tggagaacct cttctaccct gtgaccctgg atgtgctgca ccagattttc tccaagttcg
     1261 gcacagtgtt gaagatcatc accttcacca agaacaacca gttccaggcc ctgctgcagt
     1321 atgcggaccc cgtgagcgcc cagcacgcca agctgtcgct ggacgggcag aacatctaca
     1381 acgcctgctg cacgctgcgc atcgactttt ccaagctcac cagcctcaac gtcaagtaca
     1441 acaatgacaa gagccgtgac tacacacgcc cagacctgcc ttccggggac agccagccct
     1501 cgctggacca gaccatggcc gcggccttcg gtgcacctgg tataatctca gcctctccgt
     1561 atgcaggagc tggtttccct cccacctttg ccattcctca agctgcaggc ctttccgttc
     1621 cgaacgtcca cggcgccctg gcccccctgg ccatcccctc ggcggcggcg gcagctgcgg
     1681 cggcaggtcg gatcgccatc ccgggcctgg cgggggcagg aaattctgta ttgctggtca
     1741 gcaacctcaa cccagagaga gtcacacccc aaagcctctt tattcttttc ggcgtctacg
     1801 gtgacgtgca gcgcgtgaag atcctgttca ataagaagga gaacgcccta gtgcagatgg
     1861 cggacggcaa ccaggcccag ctggccatga gccacctgaa cgggcacaag ctgcacggga
     1921 agcccatccg catcacgctc tcgaagcacc agaacgtgca gctgccccgc gagggccagg
     1981 aggaccaggg cctgaccaag gactacggca actcacccct gcaccgcttc aagaagccgg
     2041 gctccaagaa cttccagaac atattcccgc cctcggccac gctgcacctc tccaacatcc
     2101 cgccctcagt ctccgaggag gatctcaagg tcctgttttc cagcaatggg ggcgtcgtca
     2161 aaggattcaa gttcttccag aaggaccgca agatggcact gatccagatg ggctccgtgg
     2221 aggaggcggt ccaggccctc attgacctgc acaaccacga cctcggggag aaccaccacc
     2281 tgcgggtctc cttctccaag tccaccatcg gatccatggt gagcggcctg ctgaaggaga
     2341 gtatgcgcat caagatgtac atggagggca ccgtgaacgg ccactacttc aagtgcgagg
     2401 gcgagggcga cggcaacccc ttcgccggca cccagagcat gagaatccac gtgaccgagg
     2461 gcgcccccct gcccttcgcc ttcgacatcc tggccccctg ctgcgagtac ggcagcagga
     2521 ccttcgtgca ccacaccgcc gagatccccg acttcttcaa gcagagcttc cccgagggct
     2581 tcacctggga gagaaccacc acctacgagg acggcggcat cctgaccgcc caccaggaca
     2641 ccagcctgga gggcaactgc ctgatctaca aggtgaaggt gcacggcacc aacttccccg
     2701 ccgacggccc cgtgatgaag aacaagagcg gcggctggga gcccagcacc gaggtggtgt
     2761 accccgagaa cggcgtgctg tgcggccgga acgtgatggc cctgaaggtg ggcgaccggc
     2821 acctgatctg ccaccactac accagctacc ggagcaagaa ggccgtgcgc gccctgacca
     2881 tgcccggctt ccacttcacc gacatccggc tccagatgct gcggaagaag aaggacgagt
     2941 acttcgagct gtacgaggcc agcgtggccc ggtacagcga cctgcccgag aaggccaact
     3001 gagttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     3061 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     3121 tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     3181 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     3241 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     3301 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     3361 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     3421 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     3481 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     3541 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact
     3601 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     3661 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     3721 cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     3781 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     3841 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     3901 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     3961 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     4021 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag
     4081 atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     4141 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     4201 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     4261 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg
     4321 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     4381 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     4441 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     4501 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     4561 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     4621 ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc
     4681 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     4741 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     4801 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     4861 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     4921 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     4981 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     5041 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa
     5101 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata
     5161 aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact
     5221 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc
     5281 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag
     5341 gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt
     5401 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     5461 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     5521 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     5581 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     5641 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     5701 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     5761 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     5821 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     5881 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     5941 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     6001 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     6061 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     6121 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     6181 cccctgattc tgtggataac cgtattaccg ccatgcat
//
