LOCUS       40924_35569        4909 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPS3081, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4909)
  AUTHORS   Kvtiko BH, Bruckbauer ST, Karkhoff-Schweizer RR, Schweizer HP.
  TITLE     Tn5/7 lux: A versatile vector for the location, capture, and 
            utilization of Gram negative promoters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4909)
  AUTHORS   Kvtiko BH, Bruckbauer ST, Karkhoff-Schweizer RR, Schweizer HP.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2013) Microbiology Immunology and Pathology, 
            Colorado State University, IDRC at Foothills Campus, Campus Delivery
            0922, Fort Collins, CO 80523-0922, USA
REFERENCE   3  (bases 1 to 4909)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4909)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-AUG-2013) Microbiology Immunology and Pathology, Colorado State 
            University, IDRC at Foothills Campus, Campus Delivery 0922, Fort 
            Collins, CO 80523-0922, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4909
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    18..65
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(175..966)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     protein_bind    1352..1399
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     mobile_element  complement(1514..1679)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     oriT            1983..2091
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      2114..2498
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             complement(2910..3767)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3768..3872)
                     /label=AmpR promoter
     misc_feature    4349..4547
                     /label=Tn7R
                     /note="Tn7R"
     primer_bind     4552..4568
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      4596..4690
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      4793..4879
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 ctcgaattgg ggatcttgaa gtacctattc cgaagttcct attctctaga aagtatagga
       61 acttcagagc gcttttgaag ctgatgtgct taaaaactta ctcaatggaa taattctaga
      121 taattcacta gtgatggaaa tgttggatcc tcatactctt cctttttcaa ttcagaagaa
      181 ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag
      241 cacgaggaag cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa
      301 cgctatgtcc tgatagcggt ccgccacacc cagccggcca cagtcgatga atccagaaaa
      361 gcggccattt tccaccatga tattcggcaa gcaggcatcg ccatgggtca cgacgagatc
      421 ctcgccgtcg ggcatgcgcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg
      481 atgctcttcg tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg
      541 ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag
      601 ccgccgcatt gcatcagcca tgatggatac tttctcggca ggagcaaggt gggatgacag
      661 gagatcctgc cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac
      721 gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc
      781 gtcctgcagt tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc
      841 ctgcgctgac agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc
      901 atagccgaat agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc
      961 aatcatgcga aacgatcctc atcctgtctc ttgatcagat cttgatcccc tgcgccatca
     1021 gatccttggc ggcaagaaag ccatccagtt tactttgcag ggcttcccaa ccttaccaga
     1081 gggcgcccca gctggcaatt ccggttcgct tgctgtccat aaaaccgccc agtctagcta
     1141 tcgccatgta agcccactgc aagctacctg ctttctcttt gcgcttgcgt tttcccttgt
     1201 ccagatagcc cagtagctga cattcatccg gggtcagcac cgtttctgcg gactggcttt
     1261 ctacgtgttc cgcttccttt agcagccctt gcgccatgca tttgttaatc gaattatcca
     1321 ttgctgttga caaagggaat caggggatct tgaagttcct attccgaagt tcctattctc
     1381 tagaaagtat aggaacttca gagcgctttt gaagctaatt cgagatcatg catgagctca
     1441 ctagtggatc ccccgggctg caggaattcc tcgagaagct tgggcccggt acctcgcgaa
     1501 ggccttgcag gccaaccaga taagtgaaat ctagttccaa actattttgt catttttaat
     1561 tttcgtatta gcttacgacg ctacacccag ttcccatcta ttttgtcact cttccctaaa
     1621 taatccttaa aaactccatt tccacccctc ccagttccca actattttgt ccgcccacag
     1681 cggggcattt ttcttcctgt tatgtttggg cgctgcatta atgaatcggc caacgcgcgg
     1741 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac ccgctgcgct
     1801 cggtcgttcg gctgcggcga gcggtatcag gggattcctt aaggtatact ttccgctgca
     1861 taaccctgct tcggggtcat tatagcgatt ttttcggtat atccatcctt tttcgcacga
     1921 tatacaggat tttgccaaag ggttcgtgta gactttcctt ggtgtatcca acggcgtcag
     1981 ccgggcagga taggtgaagt aggcccaccc gcgagcgggt gttccttctt cactgtccct
     2041 tattcgcacc tggcggtgct caacgggaat cctgctctgc gaggctggcc gataagctct
     2101 gataacgcag gaaagaacat gggcagttca acctgttgat agtacgtact aagctctcat
     2161 gtttcacgta ctaagctctc aagtttaacg tactaagctc tcatgtttaa cgaactaaac
     2221 cctcatggct aacgtactaa gctctcatgg ctaacgtact aagctctcat gtttcacgta
     2281 ctaagctctc atgtttgaac aataaaatta atataaatca gcaacttaaa tagcctctaa
     2341 ggttttaagt tttataagaa aaaaaagaat atataaggct tttaaagctt ttaaggttta
     2401 acggttgtgg acaacaagcc agggatgtaa cgcactgaga agcccttaga gcctctcaaa
     2461 gcaattttga gtgacacagg aacacttaac ggctgacaac cggctggtaa caggattagc
     2521 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     2581 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     2641 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     2701 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     2761 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     2821 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     2881 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     2941 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     3001 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     3061 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     3121 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     3181 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     3241 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     3301 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     3361 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     3421 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     3481 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     3541 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     3601 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     3661 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     3721 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     3781 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     3841 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     3901 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     3961 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     4021 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     4081 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
     4141 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     4201 aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     4261 tcgctattac gccagctaga ggaccagccg cgtaacctgg caaaatcggt tacggttgag
     4321 taataaatgg atgccctgcg taagcgggtg tgggcggaca ataaagtctt aaactgaaca
     4381 aaatagatct aaactatgac aataaagtct taaactagac agaatagttg taaactgaaa
     4441 tcagtccagt tatgctgtga aaaagcatac tggacttttg ttatggctaa agcaaactct
     4501 tcattttctg aagtgcaaat tgcccgtcgt attaaagagg ggcgtggggt tcgaggtcga
     4561 cggtatcgat aagctagctt aattagctga gcttggactc ctgttgatag atccagtaat
     4621 gacctcagaa ctccatctgg atttgttcag aacgctcggt tgccgccggg cgttttttat
     4681 tggtgagaat ccaagctaga ctgcgatgag tggcagggcg gggcgtaatt tttttaaggc
     4741 agttattggt gcccttaaac gcctggggta atgactctct agcttgaggc atcaaataaa
     4801 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     4861 tctcctgagt aggacaaatc cgccgctagg agcttgcggc ccggacgat
//
