LOCUS       40924_35564        4726 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPS2836, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Kvtiko BH, Bruckbauer ST, Karkhoff-Schweizer RR, Schweizer HP.
  TITLE     Tn5/7 lux: A versatile vector for the location, capture, and 
            utilization of Gram negative promoters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4726)
  AUTHORS   Kvtiko BH, Bruckbauer ST, Karkhoff-Schweizer RR, Schweizer HP.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2013) Microbiology Immunology and Pathology, 
            Colorado State University, IDRC at Foothills Campus, Campus Delivery
            0922, Fort Collins, CO 80523-0922, USA
REFERENCE   3  (bases 1 to 4726)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4726)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-AUG-2013) Microbiology Immunology and Pathology, Colorado State 
            University, IDRC at Foothills Campus, Campus Delivery 0922, Fort 
            Collins, CO 80523-0922, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(374..758)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     oriT            complement(781..889)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     promoter        complement(1183..1201)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1208..1224)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1366..1746
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             2114..2905
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SRLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVIHGDACLPNIMVENGRFSGFNDCGRLGVADRYQDIA
                     LDTRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             2926..3783
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCHTLLSRIDAGQEQLGRRARYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDESDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3957..4545
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgg ctttaatccc
      301 gggaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
      361 gttaccagcc ggttgtcagc cgttaagtgt tcctgtgtca ctcaaaattg ctttgagagg
      421 ctctaagggc ttctcagtgc gttacatccc tggcttgttg tccacaaccg ttaaacctta
      481 aaagctttaa aagccttata tattcttttt tttcttataa aacttaaaac cttagaggct
      541 atttaagttg ctgatttata ttaattttat tgttcaaaca tgagagctta gtacgtgaaa
      601 catgagagct tagtacgtta gccatgagag cttagtacgt tagccatgag ggtttagttc
      661 gttaaacatg agagcttagt acgttaaact tgagagctta gtacgtgaaa catgagagct
      721 tagtacgtac tatcaacagg ttgaactgcc catgttcttt cctgcgttat cagagcttat
      781 cggccagcct cgcagagcag gattcccgtt gagcaccgcc aggtgcgaat aagggacagt
      841 gaagaaggaa cacccgctcg cgggtgggcc tacttcacct atcctgcccg gctgacgccg
      901 ttggatacac caaggaaagt ctacacgaac cctttggcaa aatcctgtat atcgtgcgaa
      961 aaaggatgga tataccgaaa aaatcgctat aatgaccccg aagcagggtt atgcagcgga
     1021 aagtatacct taaggaatcc cctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     1081 ggtcagtgag cgaggaagcg gaagcccggg aataagccga attctgcaga tatccatcac
     1141 actggcggcc gctcgagcat gcatctagag ggcccaattc gccctatagt gagtcgtatt
     1201 acaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     1261 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     1321 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg
     1381 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     1441 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     1501 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagagct ttacggcacc
     1561 tcgaccgcaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga
     1621 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     1681 ctggaacaac actcaaccct atcgcggtct attcttttga tttataaggg attttgccga
     1741 tttcggccta ttggttaaaa aatgagctga tttaacaaat tcagggcgca agggctgcta
     1801 aaggaaccgg aacacgtaga aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt
     1861 cagctactgg gctatctgga caagggaaaa cgcaagcgca aagagaaagc aggtagcttg
     1921 cagtgggctt acatggcgat agctagactg ggcggtttta tggacagcaa gcgaaccgga
     1981 attgccagct ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa actggatggc
     2041 tttcttgccg ccaaggatct gatggcgcag gggatcaaga tctgatcaag agacaggatg
     2101 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     2161 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     2221 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     2281 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     2341 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     2401 agtgccgggg caggatctcc tgtcatctcg ccttgctcct gccgagaaag tatccatcat
     2461 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     2521 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     2581 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     2641 gcgcatgccc gacggcgagg atctcgtcgt gatccatggc gatgcctgct tgccgaatat
     2701 catggtggaa aatggccgct tttctggatt caacgactgt ggccggctgg gtgtggcgga
     2761 ccgctatcag gacatagcgt tggatacccg tgatattgct gaagagcttg gcggcgaatg
     2821 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     2881 ctatcgcctt cttgacgagt tcttctgaat tgaaaaagga agagtatgag tattcaacat
     2941 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     3001 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     3061 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     3121 atgatgagca cttttaaagt tctgctatgt catacactat tatcccgtat tgacgccggg
     3181 caagagcaac tcggtcgccg ggcgcggtat tctcagaatg acttggttga gtactcacca
     3241 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     3301 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     3361 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     3421 gagctgaatg aagccatacc aaacgacgag agtgacacca cgatgcctgt agcaatgcca
     3481 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     3541 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     3601 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     3661 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     3721 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     3781 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     3841 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     3901 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     3961 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     4021 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     4081 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     4141 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     4201 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     4261 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     4321 accgaactga gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga
     4381 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     4441 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     4501 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     4561 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     4621 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     4681 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaag
//
