LOCUS       40924_35559        4381 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPS2305, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4381)
  AUTHORS   Kvtiko BH, Bruckbauer ST, Karkhoff-Schweizer RR, Schweizer HP.
  TITLE     Tn5/7 lux: A versatile vector for the location, capture, and 
            utilization of Gram negative promoters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4381)
  AUTHORS   Kvtiko BH, Bruckbauer ST, Karkhoff-Schweizer RR, Schweizer HP.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2013) Microbiology Immunology and Pathology, 
            Colorado State University, IDRC at Foothills Campus, Campus Delivery
            0922, Fort Collins, CO 80523-0922, USA
REFERENCE   3  (bases 1 to 4381)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4381)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-AUG-2013) Microbiology Immunology and Pathology, Colorado State 
            University, IDRC at Foothills Campus, Campus Delivery 0922, Fort 
            Collins, CO 80523-0922, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4381
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    700..721
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        736..766
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    774..790
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     798..814
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    886..1358
                     /label=XEN5 region
                     /note="XEN5 region"
     promoter        complement(1431..1449)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1456..1472)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1614..1994
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             2362..3153
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SRLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVIHGDACLPNIMVENGRFSGFNDCGRLGVADRYQDIA
                     LDTRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3174..4031
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCHTLLSRIDAGQEQLGRRARYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDESDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4205..4381
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
       61 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
      121 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
      181 gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca cgcttcccga
      241 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
      301 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
      361 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
      421 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
      481 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
      541 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
      601 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
      661 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
      721 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
      781 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc ttggtaccga
      841 gctcggatcc actagtaacg gccgccagtg tgctggaatt cggcttcacc gcgtgtcgtt
      901 cgtcctggaa aagatgcgag gtggggcaag gctaaggtcg ctaccggtgg cggtcaaggc
      961 acctgcgcgg caactgatcg aagagccagt ccggatcgat gtgccggcga acgtgcgggg
     1021 atctgcgtct tccctggcga gagggcgccg gactggacca tgactgccgg tcgggcgggg
     1081 cgttttcccg ttggcgtatc ccgccggtgt tgattagact gccggggttc ttgtcggggt
     1141 gccctatacg aggggctgag atcggatagt tccggatccc gttgaacctg atcgggctag
     1201 cgtccggttc cgcctcgcgc ggaccgcagc aacccgcgta gggaacaaga tgtccgcgcc
     1261 atgcctgcct tgccggcgcg tccttctcgc caccagtccc ggtggccccg cgtgccaggt
     1321 tctcccgaca atcacccagg agagccatcg atcacatagt gaagccgaat tctgcagata
     1381 tccatcacac tggcggccgc tcgagcatgc atctagaggg cccaattcgc cctatagtga
     1441 gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     1501 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     1561 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     1621 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     1681 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     1741 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagagcttt
     1801 acggcacctc gaccgcaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc
     1861 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     1921 gttccaaact ggaacaacac tcaaccctat cgcggtctat tcttttgatt tataagggat
     1981 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaattc agggcgcaag
     2041 ggctgctaaa ggaaccggaa cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg
     2101 atgaatgtca gctactgggc tatctggaca agggaaaacg caagcgcaaa gagaaagcag
     2161 gtagcttgca gtgggcttac atggcgatag ctagactggg cggttttatg gacagcaagc
     2221 gaaccggaat tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac
     2281 tggatggctt tcttgccgcc aaggatctga tggcgcaggg gatcaagatc tgatcaagag
     2341 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     2401 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     2461 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     2521 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
     2581 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     2641 ttgggcgaag tgccggggca ggatctcctg tcatctcgcc ttgctcctgc cgagaaagta
     2701 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     2761 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     2821 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     2881 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga tccatggcga tgcctgcttg
     2941 ccgaatatca tggtggaaaa tggccgcttt tctggattca acgactgtgg ccggctgggt
     3001 gtggcggacc gctatcagga catagcgttg gatacccgtg atattgctga agagcttggc
     3061 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     3121 atcgccttct atcgccttct tgacgagttc ttctgaattg aaaaaggaag agtatgagta
     3181 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     3241 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     3301 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     3361 gttttccaat gatgagcact tttaaagttc tgctatgtca tacactatta tcccgtattg
     3421 acgccgggca agagcaactc ggtcgccggg cgcggtattc tcagaatgac ttggttgagt
     3481 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     3541 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     3601 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     3661 gggaaccgga gctgaatgaa gccataccaa acgacgagag tgacaccacg atgcctgtag
     3721 caatgccaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     3781 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     3841 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     3901 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     3961 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     4021 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     4081 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     4141 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     4201 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     4261 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     4321 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     4381 a
//
