LOCUS       40924_35544        6348 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Plastid transformation vector pPRV312L, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6348)
  AUTHORS   Chakrabarti SK, Lutz KA, Lertwiriyawong B, Svab Z, Maliga P.
  TITLE     Expression of the cry9Aa2 B.t. gene in tobacco chloroplasts confers 
            resistance to potato tuber moth
  JOURNAL   Transgenic Res. 15 (4), 481-488 (2006)
  PUBMED    16906448
REFERENCE   2  (bases 1 to 6348)
  AUTHORS   Maliga P, Lutz KA.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-APR-2006) Waksman Institute, Rutgers University, 190 
            Frelinghuysen Road, Piscataway, NJ 08854-8020, USA
REFERENCE   3  (bases 1 to 6348)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6348)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Transgenic 
            Res."; date: "2006"; volume: "15"; issue: "4"; pages: "481-488"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-APR-2006) Waksman Institute, Rutgers University, 190 
            Frelinghuysen Road, Piscataway, NJ 08854-8020, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6348
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..1242
                     /note="similar to Nicotiana tabacum ptDNA in GenBank
                     Accession Number Z00044; plastid sequence targeting 
                     insertions in the trnI-trnA intergenic region"
     misc_feature    1259..1315
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     misc_feature    1332..1365
                     /label=P1 phage loxP
                     /note="P1 phage loxP"
     protein_bind    1332..1365
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    1372..1544
                     /note="tobacco plastid rRNA operon promoter (Prrn) fused
                     with tobacco plastid atpB gene leader (described in Kuroda 
                     and Maliga, Plant Phys 125:430-436, 2001)"
     regulatory      1372..1455
                     /label=tobacco plastid rRNA operon promoter (Prrn)
                     /note="tobacco plastid rRNA operon promoter (Prrn)"
                     /regulatory_class="promoter"
     misc_feature    1455..1544
                     /label=tobacco plastid atpB gene leader
                     /note="tobacco plastid atpB gene leader"
     misc_feature    1545..1586
                     /gene="atpB-aad-c-myc"
                     /label=N-terminus of tobacco plastid AtpB
                     /note="N-terminus of tobacco plastid AtpB"
     CDS             1596..2378
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     CDS             2379..2408
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     misc_feature    2425..2613
                     /note="similar to tobacco plastid psbA 3' UTR in GenBank 
                     Accession Number Z00044"
     protein_bind    complement(2632..2665)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    2674..2681
                     /label=contains SwaI restriction site
                     /note="contains SwaI restriction site"
     misc_feature    2685..3508
                     /note="similar to Nicotiana tabacum ptDNA in GenBank
                     Accession Number Z00044; plastid sequence targeting 
                     insertions in the trnI-trnA intergenic region"
     rep_origin      3651..4106
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4388..4492
                     /label=AmpR promoter
     CDS             4493..5350
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5524..6112
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gggccttgta cacaccgccc gtcacactat gggagctggc catgcccgaa gtcgttacct
       61 taaccgcaag gagggggatg ccgaaggcag ggctagtgac tggagtgaag tcgtaacaag
      121 gtagccgtac tggaaggtgc ggctggatca cctccttttc agggagagct aatgcttgtt
      181 gggtattttg gtttgacact gcttcacacc cccaaaaaaa agaagggagc tacgtctgag
      241 ttaaacttgg agatggaagt cttctttcct ttctcgacgg tgaagtaaga ccaagctcat
      301 gagcttatta tcctaggtcg gaacaagttg ataggacccc cttttttacg tccccatgtt
      361 ccccccgtgt ggcgacatgg gggcgaaaaa aggaaagaga gggatggggt ttctctcgct
      421 tttggcatag cgggccccca gtgggaggct cgcacgacgg gctattagct cagtggtaga
      481 gcgcgcccct gataattgcg tcgttgtgcc tgggctgtga gggctctcag ccacatggat
      541 agttcaatgt gctcatcggc gcctgaccct gagatgtgga tcatccaagg cacattagca
      601 tggcgtactc ctcctgttcg aaccggggtt tgaaaccaaa ctcctcctca ggaggataga
      661 tggggcgatt cgggtgagat ccaatgtaga tccaactttc gattcactcg tgggatccgg
      721 gcggtccggg ggggaccacc acggctcctc tcttctcgag aatccataca tcccttatca
      781 gtgtatggac agctatctct cgagcacagg tttagcaatg ggaaaataaa atggagcacc
      841 taacaacgca tcttcacaga ccaagaacta cgagatcgcc cctttcattc tggggtgacg
      901 gagggatcgt accattcgag ccgttttttt cttgactcga aatgggagca ggtttgaaaa
      961 aggatcttag agtgtctagg gttgggccag gagggtctct taacgccttc ttttttcttc
     1021 tcatcggagt tatttcacaa agacttgcca gggtaaggaa gaagggggga acaagcacac
     1081 ttggagagcg cagtacaacg gagagttgta tgctgcgttc gggaaggatg aatcgctccc
     1141 gaaaaggaat ctattgattc tctcccaatt ggttggaccg taggtgcgat gatttacttc
     1201 acgggcgagg tctctggttc aagtccagga tggcccagct gcatttaaat ggcgcgccga
     1261 attcgagctc ggtacccggg gatcctctag agtcgacctg caggcatgca agcttgcggc
     1321 cgcagtagct tataacttcg tatagcatac attatacgaa gttatagatc cgctcccccg
     1381 ccgtcgttca atgagaatgg ataagaggct cgtgggattg acgtgagggg gcagggatgg
     1441 ctatatttct gggagaatta accgatcgac gtgcaagcgg acatttattt taaattcgat
     1501 aatttttgca aaaacatttc gacatattta tttattttat tattatgaga atcaatccta
     1561 ctacttctgg ttctggggtt tccacggcta gtagcgaagc ggtgatcgcc gaagtatcga
     1621 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
     1681 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
     1741 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
     1801 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
     1861 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
     1921 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
     1981 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
     2041 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     2101 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     2161 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     2221 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
     2281 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc
     2341 actacgtgaa aggcgagatc accaaggtag tgggcaaaga acaaaaactc atttctgaag
     2401 aagacttgtg agtctagcta gagcgatcct ggcctagtct ataggaggtt ttgaaaagaa
     2461 aggagcaata atcattttct tgttctatca agagggtgct attgctcctt tctttttttc
     2521 tttttattta tttactagta ttttacttac atagactttt ttgtttacat tatagaaaaa
     2581 gaaggagagg ttattttctt gcatttattc atgggggatc aaagctgatc tataacttcg
     2641 tatagcatac attatacgaa gttatggtac actatttaaa tgcgctccac ttggctcggg
     2701 gggatatagc tcagttggta gagctccgct cttgcaattg ggtcgttgcg attacgggtt
     2761 ggatgtctaa ttgtccaggc ggtaatgata gtatcttgta cctgaaccgg tggctcactt
     2821 tttctaagta atggggaaga ggaccgaaac gtgccactga aagactctac tgagacaaag
     2881 atgggctgtc aagaacgtag aggaggtagg atgggcagtt ggtcagatct agtatggatc
     2941 gtacatggac ggtagttgga gtcggcggct ctcccagggt tccctcatct gagatctctg
     3001 gggaagagga tcaagttggc ccttgcgaac agcttgatgc actatctccc ttcaaccctt
     3061 tgagcgaaat gcggcaaaag aaaaggaagg aaaatccatg gaccgacccc atcatctcca
     3121 ccccgtagga actacgagat caccccaagg acgccttcgg catccagggg tcacggaccg
     3181 accatagaac cctgttcaat aagtggaacg cattagctgt ccgctctcag gttgggcagt
     3241 cagggtcgga gaagggcaat gactcattct tagttagaat gggattccaa ctcagcacct
     3301 tttgagtgag attttgagaa gagttgctct ttggagagca cagtacgatg aaagttgtaa
     3361 gctgtgttcg ggggggagtt attgtctatc gttggcctct atggtagaat cagtcggggg
     3421 acctgagagg cggtggttta ccctgcggcg gatgtcagcg gttcgagtcc gcttatctcc
     3481 aactcgtgaa cttagccgat acaaagctct ggcgtaatag cgaagaggcc cgcaccgatc
     3541 gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc
     3601 ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg
     3661 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     3721 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     3781 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     3841 gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg
     3901 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     3961 ccaaactgga acaacactca accctatctc gggctattct tttgatttat aagggatttt
     4021 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     4081 taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc
     4141 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     4201 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     4261 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     4321 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     4381 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     4441 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     4501 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     4561 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     4621 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     4681 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     4741 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     4801 ttcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     4861 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     4921 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     4981 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     5041 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     5101 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     5161 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     5221 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     5281 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     5341 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     5401 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     5461 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     5521 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     5581 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     5641 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     5701 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     5761 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     5821 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     5881 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     5941 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     6001 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     6061 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     6121 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     6181 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     6241 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     6301 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcag
//
