LOCUS       40924_35539        4733 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPROEX-1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Polayes D.
  TITLE     Plasmid pPROEX - cloning vector for protein expression and 
            purification
  JOURNAL   Unpublished
REFERENCE   2  (bases 2564 to 2571)
  AUTHORS   Polayes D, Hughes AJohn.
  TITLE     Efficient protein expression and simple purification using the 
            pPROEX-1 system
  JOURNAL   Focus 16, 81-84 (1994)
REFERENCE   3  (bases 1 to 4733)
  AUTHORS   Horton ME.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JAN-1995) Mary E. Horton, Technical Services, Life 
            Technologies, Inc., 8400 Helgerman Court, Gaithersburg, MD 
            20884-9980, USA
REFERENCE   4  (bases 1 to 4733)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 4733)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Focus 16, 
            81-84 (1994)"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (09-JAN-1995) Mary E. Horton, Technical Services, Life Technologies,
            Inc., 8400 Helgerman Court, Gaithersburg, MD 20884-9980, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4733
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    230..246
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             273..290
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             312..332
                     /codon_start=1
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
                     /translation="ENLYFQG"
     terminator      625..711
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      803..830
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        850..941
                     /label=AmpR promoter
     CDS             942..1799
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPTAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1973..2561
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      2564..2571
                     /standard_name="ORI Escherichia coli pMB1"
                     /label=ORI Escherichia coli pMB1
                     /citation=[2]
                     /experiment="experimental evidence, no additional details
                     recorded"
     rep_origin      complement(2809..3237)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3534..3611
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             3612..4691
                     /codon_start=1
                     /label=lacI
                     /note="lac repressor"
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc
       61 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc
      121 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc
      181 tgaaatgagc tgttgacaat taatcatccg gtccgtataa tctgtggaat tgtgagcgga
      241 taacaatttc acacaggaaa cagaccatgg gtcatcatca tcatcatcac gattacgata
      301 tcccaacgac cgaaaacctg tattttcagg gcgcccatat gggaattcaa aggcctacgt
      361 cgacgagctc actagtcgcg gccgcttcta gaggatccct cgaggcatgc ggtaccaagc
      421 ttggctgttt tggcggatga gagaagattt tcagcctgat acagattaaa tcagaacgca
      481 gaagcggtct gataaaacag aatttgcctg gcggcagtag cgcggtggtc ccacctgacc
      541 ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg tctccccatg
      601 cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
      661 tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga
      721 gcggatttga acgttgcgaa gcaacggccc ggagggtggc gggcaggacg cccgccataa
      781 actgccaggc atcaaattaa gcagaaggcc atcctgacgg atggcctttt tgcgtttcta
      841 caaactcttt ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
      901 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
      961 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     1021 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     1081 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     1141 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag
     1201 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     1261 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     1321 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     1381 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     1441 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctacagca atggcaacaa
     1501 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     1561 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     1621 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     1681 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     1741 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     1801 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     1861 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     1921 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     1981 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     2041 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     2101 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     2161 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     2221 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     2281 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     2341 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     2401 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     2461 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2521 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     2581 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     2641 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     2701 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt
     2761 ttctccttac gcatctgtgc ggtatttcac accgcataat tttgttaaaa ttcgcgttaa
     2821 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     2881 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     2941 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     3001 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     3061 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     3121 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     3181 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcca
     3241 ttcgccattc aggctgctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     3301 aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg
     3361 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     3421 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     3481 gcgaggcagc agatcaattc gcgcgcgaag gcgaagcggc atgcatttac gttgacacca
     3541 tcgaatggtg caaaaccttt cgcggtatgg catgatagcg cccggaagag agtcaattca
     3601 gggtggtgaa tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt
     3661 atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa
     3721 aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg
     3781 cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt
     3841 cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt
     3901 cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc
     3961 aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg
     4021 aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca
     4081 acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat
     4141 tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc
     4201 gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg
     4261 aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca
     4321 tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca
     4381 ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg
     4441 aagacagctc atgttatatc ccgccgtcaa ccaccatcaa acaggatttt cgcctgctgg
     4501 ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc
     4561 agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcacccaat acgcaaaccg
     4621 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     4681 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc gcgaattgat ctg
//
