LOCUS       V003921                 9686 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003921
VERSION     V003921
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9686)
  AUTHORS   Miller WG, Leveau JH, Lindow SE.
  TITLE     Improved gfp and inaZ broad-host-range promoter-probe vectors
  JOURNAL   Mol. Plant Microbe Interact. 13 (11), 1243-1250 (2000)
   PUBMED   11059491
REFERENCE   2  (bases 1 to 9686)
  AUTHORS   Leveau JHJ., Miller WG, Lindow SE.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 9686)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9686)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Plant
            Microbe Interact."; date: "2000"; volume: "13"; issue: "11"; pages:
            "1243-1250"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (13-JUL-2000) Plant "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9686
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1023..1792
                     /label="pBBR1 oriV"
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1
                     Rep protein for replication"
     CDS             1793..2452
                     /label="pBBR1 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     primer_bind     3162..3178
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        3188..3206
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      3247..3333
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     regulatory      3420..3592
                     /label="T1 rrnB terminator region"
                     /note="T1 rrnB terminator region"
                     /regulatory_class="terminator"
     terminator      3428..3514
                     /gene="Escherichia coli rrnB"
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     regulatory      3601..3773
                     /label="T1 rrnB terminator region"
                     /note="T1 rrnB terminator region"
                     /regulatory_class="terminator"
     terminator      3609..3695
                     /gene="Escherichia coli rrnB"
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     regulatory      3782..3954
                     /label="T1 rrnB terminator region"
                     /note="T1 rrnB terminator region"
                     /regulatory_class="terminator"
     terminator      3790..3876
                     /gene="Escherichia coli rrnB"
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    complement(3968..4024)
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     regulatory      4047..4051
                     /label="putative"
                     /note="putative"
                     /regulatory_class="ribosome_binding_site"
     CDS             4059..7658
                     /gene="inaZ"
                     /label="Ice nucleation protein"
                     /note="Ice nucleation protein from Pseudomonas syringae pv.
                     syringae. Accession#: P06620"
     terminator      complement(7682..7725)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     promoter        complement(7877..7895)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7916..7932)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(7940..7956)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7964..7994)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8009..8030)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(8438..9229)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat ctcacgcgct cctgcggcgg
       61 cctgtagggc aggctcatac ccctgccgaa ccgcttttgt cagccggtcg gccacggctt
      121 ccggcgtctc aacgcgcttt gagattccca gcttttcggc caatccctgc ggtgcatagg
      181 cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc cactggtggc cgctccaggg
      241 cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct gccctcgatg ccccgttgca
      301 gccctagatc ggccacagcg gccgcaaacg tggtctggtc gcgggtcatc tgcgctttgt
      361 tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt cagcggcacc acgaacgcgg
      421 tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt cacgatgcga tccgccccgt
      481 acttgtccgc cagccacttg tgcgccttct cgaagaacgc cgcctgctgt tcttggctgg
      541 ccgacttcca ccattccggg ctggccgtca tgacgtactc gaccgccaac acagcgtcct
      601 tgcgccgctt ctctggcagc aactcgcgca gtcggcccat cgcttcatcg gtgctgctgg
      661 ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc gttgggcgtc tcgcgctcgc
      721 ggtaggcgtg cttgagactg gccgccacgt tgcccatttt cgccagcttc ttgcatcgca
      781 tgatcgcgta tgccgccatg cctgcccctc ccttttggtg tccaaccggc tcgacggggg
      841 cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg agtatactca ctagactttg
      901 cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc tacgggcttg ctctccgggc
      961 ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga cgatggccgc
     1021 gagcggccac cggctggctc gcttcgctcg gcccgtggac aaccctgctg gacaagctga
     1081 tggacaggct gcgcctgccc acgagcttga ccacagggat tgcccaccgg ctacccagcc
     1141 ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg gccttgcccc atcaattttt
     1201 ttaattttct ctggggaaaa gcctccggcc tgcggcctgc gcgcttcgct tgccggttgg
     1261 acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg cgcagcggct tggccttgac
     1321 gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc gaaggcgaag cccgcccgcc
     1381 tgccccccga gcctcacggc ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg
     1441 caaggccgaa ggccgcgcag tcgatcaaca agccccggag gggccacttt ttgccggagg
     1501 gggagccgcg ccgaaggcgt gggggaaccc cgcaggggtg cccttctttg ggcaccaaag
     1561 aactagatat agggcgaaat gcgaaagact taaaaatcaa caacttaaaa aaggggggta
     1621 cgcaacagct cattgcggca ccccccgcaa tagctcattg cgtaggttaa agaaaatctg
     1681 taattgactg ccacttttac gcaacgcata attgttgtcg cgctgccgaa aagttgcagc
     1741 tgattgcgca tggtgccgca accgtgcggc accctaccgc atggagataa gcatggccac
     1801 gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga
     1861 acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct
     1921 gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac
     1981 actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt
     2041 ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt
     2101 ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag
     2161 tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct
     2221 gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga
     2281 gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac
     2341 ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct
     2401 ggacgatggc gagccgttgg agccgccgac acgggtcacg ctgccgcgcc ggtagcactt
     2461 gggttgcgca gcaacccgta agtgcgctgt tccagactat cggctgtagc cgcctcgccg
     2521 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga
     2581 cctgaatgga agccggcggc acctcgctaa cggattcacc gtttttatca ggctctggga
     2641 ggcagaataa atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg
     2701 gcctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa
     2761 ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa
     2821 tttgctttcg aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaccag
     2881 gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc
     2941 agtcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     3001 aaatattaac gcttacaatt tccattcgcc attcaggctg cgcaactgtt gggaagggcg
     3061 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     3121 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     3181 gcgcgcgtaa tacgactcac tatagggcga attggagctg gggccgcaat tcccaattcc
     3241 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     3301 ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg
     3361 aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag gaattaattc
     3421 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     3481 tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ggagcggatt tgaacgttgc
     3541 gaagcaacgg cccggagggt ggcgggcagg acgcccgcca taaactgcca ggaattaatt
     3601 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     3661 gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg
     3721 cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggaattaat
     3781 tccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     3841 tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgggagcgga tttgaacgtt
     3901 gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc cataaactgc caggaattgg
     3961 ggatcggaag cttgcatgcc tgcaggtcga ctctagagga tccccgggta ccgagctcga
     4021 attcggctta accccaaagg atctatgagg atgctgtaat gaatctcgac aaggcgttgg
     4081 tgctgcgtac ctgtgcaaat aacatggccg atcactgcgg ccttatatgg cccgcgtccg
     4141 gcacggtgga atccagatac tggcagtcaa ccaggcggca tgagaatggt ctggtcggtt
     4201 tactgtgggg cgctggaacc agcgcttttc taagcgtgca tgccgatgct cgatggattg
     4261 tctgtgaagt tgccgttgca gacatcatca gtctggaaga gccgggaatg gtcaagtttc
     4321 cgcgggccga ggtggttcat gtcggcgaca ggatcagcgc gtcacacttc atttcggcac
     4381 gtcaggccga ccctgcgtca acatcaacat caacgttaac gccaatgccc actgccatac
     4441 ccacgcccat gcctgccgta gcaagtgtca cgttaccggt ggccgaacag gcccgtcatg
     4501 aagtgttcga tgtcgcgtcg gtcagcgcgg ctgccgcccc agtaaacacc ctgccggtga
     4561 cgacgccgca gaatgtgcag accgccactt acggcagcac gttgagtggc gacaatcaca
     4621 gtcgtctgat tgccggttat ggcagtaacg agaccgctgg caaccacagt gatctaattg
     4681 ccggttatgg aagtacaggc accgccggct ccgacagctg gctggtcgct ggctatggaa
     4741 gcacccagac cgccggtggg gacagcgcgc tgacagcggg ttacggcagc acccagaccg
     4801 cccgcgaagg cagcaacctg acggcagggt acggcagcac cggcacggca ggctcggaca
     4861 gttcgctgat cgccggttac ggcagtactc agacttcggg cggggacagc tcactcacag
     4921 cgggttacgg cagcacgcaa acggctcagg aaggcagcaa tctcaccgct gggtatggca
     4981 gcaccggcac ggcaggctcg gacagctcct tgatcgccgg ttatggcagt acacaaacct
     5041 cgggaggcga cagttcgctg accgcgggct acggcagtac gcagacggcc caggagggca
     5101 gcaatctgac ggcggggtac ggcagcacgg gtacagcagg tgtcgacagc tctctgatcg
     5161 cgggatacgg cagcacgcag acctcgggaa gtgacagcgc cctgaccgca ggctatggca
     5221 gcacgcaaac ggcccaggaa ggcagcaatc tcactgctgg gtatggcagc accggcacgg
     5281 caggttccga cagctcgctg atcgccggtt acggcagcac gcaaacctcg ggcagtgaca
     5341 gctcgctcac ggcggggtac ggcagtacgc agacggctca ggaaggcagc attctgacgg
     5401 cggggtacgg cagcacgggt acagcaggtg tcgacagttc gttgatcgcc ggatatggca
     5461 gcacgcagac ctcgggaagt gacagtgcgc tgacagcggg ttacggcagc acgcaaacgg
     5521 cccaggaagg cagcaacctg acggcgggct acggcagcac tggcacggca ggtgccgaca
     5581 gttcgttgat cgccggatat ggcagcacgc agacgtcagg cagcgaaagt tcgcttaccg
     5641 caggctatgg cagtacccag actgcccgtg agggcagcac cctgacggcc ggatatggca
     5701 gtaccggaac agctggcgct gacagctcgc tgatcgccgg ttacggcagc acgcaaacct
     5761 cgggcagtga aagctcgctc acggcaggtt atggcagtac ccagaccgca cagcagggca
     5821 gcgtactcac atcaggctat ggcagtacgc aaacggccgg ggctgccagt aacctcacca
     5881 ccggttacgg aagtacaggt accgcaggtc acgagagttt catcattgcg ggttatggaa
     5941 gtacacagac agcgggccac aaaagtatcc tgaccgctgg ttatggcagt actcagacgg
     6001 ccagggacgg tagcgacctg attgcgggct atggcagtac gggaaccgca ggctcgggca
     6061 gttcgctgat cgcaggttat ggcagcaccc agaccgcgag ttacagaagc atgctgaccg
     6121 ccggttatgg cagtacccag accgccagag aacacagcga ccttgtcaca ggctatggca
     6181 gcacttcaac ggcagggtca aacagttcgc tgatcgccgg ctatggaagc actcagacgg
     6241 cgggcttcaa aagcatactg accgccggtt acggcagtac ccagacggca caggagcgca
     6301 cgagcctggt cgcaggctac ggaagcacgt cgactgcggg ctattccagt tccttgatcg
     6361 ccggctatgg cagcacgcag acggcaggct acgaaagcac gttgaccgcc ggttacggca
     6421 gtacgcaaac cgctcaggaa aacagctcgc tcaccacagg ttacggaagt acttctactg
     6481 cgggctattc cagctcgctc atcgcgggtt acggcagtac gcaaacggca ggctacgaga
     6541 gcacgttgac cgccggttac ggtagtacgc aaaccgcgca ggagcgcagt gatctggtga
     6601 caggttatgg aagtacctcc accgccggct atgcgagctc gctgattgcg ggttatggca
     6661 gcacgcagac tgcgggttat gagagcacgt tgaccgccgg ttacggcagc acgcaaaccg
     6721 cacaggaaaa cagctcgctc accaccgggt acggaagtac ctccacagcc ggctttgcca
     6781 gctcgctgat ctccggttat ggcagtacgc agacagccgg ctataaaagt accctcacgg
     6841 ccggttacgg cagtactcag accgcagagt atggaagctc actcactgcg ggctacggca
     6901 gcactgcaac ggccgggcag gacagttcat tgatagccgg ctatggcagc tccctgacca
     6961 gcggaatcag aagttttctg acggcaggct atggcagtac gctgatcgcc ggacttcgca
     7021 gcgttttgat cgccggttat ggcagtagtc ttacatcggg cgttcgcagc acgttgactg
     7081 cgggttatgg cagtaaccag attgcaagtt acggcagctc gttgattgca ggccatgaaa
     7141 gcattcaggt cgccggaaat aaaagcatgc tgatcgccgg caagggcagc tcgcagacag
     7201 caggttttcg cagcacgctg attgccggtg cgggcagtgt acaactggcg ggtgatcgca
     7261 gccggttgat tgccggtgca gacagtaatc agaccgcggg tgaccgcagc aaactgctgg
     7321 ccggtaataa cagttatctg actgccggcg atagaagcaa actgaccggc gggcatgact
     7381 gcaccctgat ggcgggagac caaagcagat tgaccgctgg taagaacagt gtcttgacgg
     7441 caggcgctcg tagcaaactg attggcagtg aaggctcgac gctctcggct ggagaagact
     7501 ccatacttat tttcaggctc tgggacggga agaggtacag gcaactggtc gccagaacgg
     7561 gtgagaacgg tgttgaggcc gacataccgt attacgtgaa cgaagatgac gatattgtcg
     7621 ataaacccga cgaggacgat gactggatag aggtaaagta gcccgcaatt ccaggcatca
     7681 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     7741 gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg
     7801 gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggaattggg ggcggccccg
     7861 tacccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat
     7921 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     7981 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     8041 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     8101 aacgcgcggg gagaggcggt ttgcgtattg ggcgcatgca taaaaactgt tgtaattcat
     8161 taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg
     8221 gcatcagcac cttgtcgcct tgcgtataat atttgcccat gggggtgggc gaagaactcc
     8281 agcatgagat ccccgcgctg gaggatcatc cagccggcgt cccggaaaac gattccgaag
     8341 cccaaccttt catagaaggc ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc
     8401 gcttggtcgg tcatttcgaa ccccagagtc ccgctcagaa gaactcgtca agaaggcgat
     8461 agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag
     8521 cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc
     8581 ggtccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca
     8641 tgatattcgg caagcaggca tcgccatggg tcacgacgag atcctcgccg tcgggcatgc
     8701 gcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct tcgtccagat
     8761 catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg
     8821 cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag
     8881 ccatgatgga tactttctcg gcaggagcaa ggtgagatga caggagatcc tgccccggca
     8941 cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc
     9001 aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc ctcgtcctgc agttcattca
     9061 gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga
     9121 acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg aatagcctct
     9181 ccacccaagc ggccggagaa cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc
     9241 ctcatcctgt ctcttgatca gatcttgatc ccctgcgcca tcagatcctt ggcggcaaga
     9301 aagccatcca gtttactttg cagggcttcc caaccttacc agagggcgcc ccagctggca
     9361 attccggttc gcttgctgtc cataaaaccg cccagtctag ctatcgccat gtaagcccac
     9421 tgcaagctac ctgctttctc tttgcgcttg cgttttccct tgtccagata gcccagtagc
     9481 tgacattcat cccaggtggc acttttcggg gaaatgtgcg cgcccgcgtt cctgctggcg
     9541 ctgggcctgt ttctggcgct ggacttcccg ctgttccgtc agcagctttt cgcccacggc
     9601 cttgatgatc gcggcggcct tggcctgcat atcccgattc aacggcccca gggcgtccag
     9661 aacgggcttc aggcgctccc gaaggt
//
