LOCUS       V003922                 9689 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003922
VERSION     V003922
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9689)
  AUTHORS   Miller WG, Leveau JH, Lindow SE.
  TITLE     Improved gfp and inaZ broad-host-range promoter-probe vectors
  JOURNAL   Mol. Plant Microbe Interact. 13 (11), 1243-1250 (2000)
   PUBMED   11059491
REFERENCE   2  (bases 1 to 9689)
  AUTHORS   Leveau JHJ., Miller WG, Lindow SE.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 9689)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9689)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Plant
            Microbe Interact."; date: "2000"; volume: "13"; issue: "11"; pages:
            "1243-1250"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (13-JUL-2000) Plant "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9689
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1023..1792
                     /label="pBBR1 oriV"
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1
                     Rep protein for replication"
     CDS             1793..2452
                     /label="pBBR1 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     primer_bind     3162..3178
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        3188..3206
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      3247..3333
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     regulatory      3420..3592
                     /label="T1 rrnB terminator region"
                     /note="T1 rrnB terminator region"
                     /regulatory_class="terminator"
     terminator      3428..3514
                     /gene="Escherichia coli rrnB"
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     regulatory      3601..3773
                     /label="T1 rrnB terminator region"
                     /note="T1 rrnB terminator region"
                     /regulatory_class="terminator"
     terminator      3609..3695
                     /gene="Escherichia coli rrnB"
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     regulatory      3782..3954
                     /label="T1 rrnB terminator region"
                     /note="T1 rrnB terminator region"
                     /regulatory_class="terminator"
     terminator      3790..3876
                     /gene="Escherichia coli rrnB"
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    3973..4022
                     /note="multicloning site; EcoRI to HindIII"
     regulatory      4050..4054
                     /label="putative"
                     /note="putative"
                     /regulatory_class="ribosome_binding_site"
     CDS             4062..7661
                     /gene="inaZ"
                     /label="Ice nucleation protein"
                     /note="Ice nucleation protein from Pseudomonas syringae pv.
                     syringae. Accession#: P06620"
     terminator      complement(7685..7728)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     promoter        complement(7880..7898)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7919..7935)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(7943..7959)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7967..7997)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8012..8033)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(8441..9232)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat ctcacgcgct cctgcggcgg
       61 cctgtagggc aggctcatac ccctgccgaa ccgcttttgt cagccggtcg gccacggctt
      121 ccggcgtctc aacgcgcttt gagattccca gcttttcggc caatccctgc ggtgcatagg
      181 cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc cactggtggc cgctccaggg
      241 cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct gccctcgatg ccccgttgca
      301 gccctagatc ggccacagcg gccgcaaacg tggtctggtc gcgggtcatc tgcgctttgt
      361 tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt cagcggcacc acgaacgcgg
      421 tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt cacgatgcga tccgccccgt
      481 acttgtccgc cagccacttg tgcgccttct cgaagaacgc cgcctgctgt tcttggctgg
      541 ccgacttcca ccattccggg ctggccgtca tgacgtactc gaccgccaac acagcgtcct
      601 tgcgccgctt ctctggcagc aactcgcgca gtcggcccat cgcttcatcg gtgctgctgg
      661 ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc gttgggcgtc tcgcgctcgc
      721 ggtaggcgtg cttgagactg gccgccacgt tgcccatttt cgccagcttc ttgcatcgca
      781 tgatcgcgta tgccgccatg cctgcccctc ccttttggtg tccaaccggc tcgacggggg
      841 cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg agtatactca ctagactttg
      901 cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc tacgggcttg ctctccgggc
      961 ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga cgatggccgc
     1021 gagcggccac cggctggctc gcttcgctcg gcccgtggac aaccctgctg gacaagctga
     1081 tggacaggct gcgcctgccc acgagcttga ccacagggat tgcccaccgg ctacccagcc
     1141 ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg gccttgcccc atcaattttt
     1201 ttaattttct ctggggaaaa gcctccggcc tgcggcctgc gcgcttcgct tgccggttgg
     1261 acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg cgcagcggct tggccttgac
     1321 gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc gaaggcgaag cccgcccgcc
     1381 tgccccccga gcctcacggc ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg
     1441 caaggccgaa ggccgcgcag tcgatcaaca agccccggag gggccacttt ttgccggagg
     1501 gggagccgcg ccgaaggcgt gggggaaccc cgcaggggtg cccttctttg ggcaccaaag
     1561 aactagatat agggcgaaat gcgaaagact taaaaatcaa caacttaaaa aaggggggta
     1621 cgcaacagct cattgcggca ccccccgcaa tagctcattg cgtaggttaa agaaaatctg
     1681 taattgactg ccacttttac gcaacgcata attgttgtcg cgctgccgaa aagttgcagc
     1741 tgattgcgca tggtgccgca accgtgcggc accctaccgc atggagataa gcatggccac
     1801 gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga
     1861 acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct
     1921 gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac
     1981 actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt
     2041 ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt
     2101 ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag
     2161 tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct
     2221 gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga
     2281 gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac
     2341 ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct
     2401 ggacgatggc gagccgttgg agccgccgac acgggtcacg ctgccgcgcc ggtagcactt
     2461 gggttgcgca gcaacccgta agtgcgctgt tccagactat cggctgtagc cgcctcgccg
     2521 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga
     2581 cctgaatgga agccggcggc acctcgctaa cggattcacc gtttttatca ggctctggga
     2641 ggcagaataa atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg
     2701 gcctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa
     2761 ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa
     2821 tttgctttcg aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaccag
     2881 gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc
     2941 agtcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     3001 aaatattaac gcttacaatt tccattcgcc attcaggctg cgcaactgtt gggaagggcg
     3061 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     3121 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     3181 gcgcgcgtaa tacgactcac tatagggcga attggagctg gggccgcaat tcccaattcc
     3241 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     3301 ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg
     3361 aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag gaattaattc
     3421 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     3481 tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ggagcggatt tgaacgttgc
     3541 gaagcaacgg cccggagggt ggcgggcagg acgcccgcca taaactgcca ggaattaatt
     3601 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     3661 gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg
     3721 cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggaattaat
     3781 tccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     3841 tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgggagcgga tttgaacgtt
     3901 gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc cataaactgc caggaattgg
     3961 ggatcggaag ctgaattcga gctcaggcct tggtacccgg gatccgtcga ctgcagaagc
     4021 ttaattcggc ttaaccccaa aggatctatg aggatgctgt aatgaatctc gacaaggcgt
     4081 tggtgctgcg tacctgtgca aataacatgg ccgatcactg cggccttata tggcccgcgt
     4141 ccggcacggt ggaatccaga tactggcagt caaccaggcg gcatgagaat ggtctggtcg
     4201 gtttactgtg gggcgctgga accagcgctt ttctaagcgt gcatgccgat gctcgatgga
     4261 ttgtctgtga agttgccgtt gcagacatca tcagtctgga agagccggga atggtcaagt
     4321 ttccgcgggc cgaggtggtt catgtcggcg acaggatcag cgcgtcacac ttcatttcgg
     4381 cacgtcaggc cgaccctgcg tcaacatcaa catcaacgtt aacgccaatg cccactgcca
     4441 tacccacgcc catgcctgcc gtagcaagtg tcacgttacc ggtggccgaa caggcccgtc
     4501 atgaagtgtt cgatgtcgcg tcggtcagcg cggctgccgc cccagtaaac accctgccgg
     4561 tgacgacgcc gcagaatgtg cagaccgcca cttacggcag cacgttgagt ggcgacaatc
     4621 acagtcgtct gattgccggt tatggcagta acgagaccgc tggcaaccac agtgatctaa
     4681 ttgccggtta tggaagtaca ggcaccgccg gctccgacag ctggctggtc gctggctatg
     4741 gaagcaccca gaccgccggt ggggacagcg cgctgacagc gggttacggc agcacccaga
     4801 ccgcccgcga aggcagcaac ctgacggcag ggtacggcag caccggcacg gcaggctcgg
     4861 acagttcgct gatcgccggt tacggcagta ctcagacttc gggcggggac agctcactca
     4921 cagcgggtta cggcagcacg caaacggctc aggaaggcag caatctcacc gctgggtatg
     4981 gcagcaccgg cacggcaggc tcggacagct ccttgatcgc cggttatggc agtacacaaa
     5041 cctcgggagg cgacagttcg ctgaccgcgg gctacggcag tacgcagacg gcccaggagg
     5101 gcagcaatct gacggcgggg tacggcagca cgggtacagc aggtgtcgac agctctctga
     5161 tcgcgggata cggcagcacg cagacctcgg gaagtgacag cgccctgacc gcaggctatg
     5221 gcagcacgca aacggcccag gaaggcagca atctcactgc tgggtatggc agcaccggca
     5281 cggcaggttc cgacagctcg ctgatcgccg gttacggcag cacgcaaacc tcgggcagtg
     5341 acagctcgct cacggcgggg tacggcagta cgcagacggc tcaggaaggc agcattctga
     5401 cggcggggta cggcagcacg ggtacagcag gtgtcgacag ttcgttgatc gccggatatg
     5461 gcagcacgca gacctcggga agtgacagtg cgctgacagc gggttacggc agcacgcaaa
     5521 cggcccagga aggcagcaac ctgacggcgg gctacggcag cactggcacg gcaggtgccg
     5581 acagttcgtt gatcgccgga tatggcagca cgcagacgtc aggcagcgaa agttcgctta
     5641 ccgcaggcta tggcagtacc cagactgccc gtgagggcag caccctgacg gccggatatg
     5701 gcagtaccgg aacagctggc gctgacagct cgctgatcgc cggttacggc agcacgcaaa
     5761 cctcgggcag tgaaagctcg ctcacggcag gttatggcag tacccagacc gcacagcagg
     5821 gcagcgtact cacatcaggc tatggcagta cgcaaacggc cggggctgcc agtaacctca
     5881 ccaccggtta cggaagtaca ggtaccgcag gtcacgagag tttcatcatt gcgggttatg
     5941 gaagtacaca gacagcgggc cacaaaagta tcctgaccgc tggttatggc agtactcaga
     6001 cggccaggga cggtagcgac ctgattgcgg gctatggcag tacgggaacc gcaggctcgg
     6061 gcagttcgct gatcgcaggt tatggcagca cccagaccgc gagttacaga agcatgctga
     6121 ccgccggtta tggcagtacc cagaccgcca gagaacacag cgaccttgtc acaggctatg
     6181 gcagcacttc aacggcaggg tcaaacagtt cgctgatcgc cggctatgga agcactcaga
     6241 cggcgggctt caaaagcata ctgaccgccg gttacggcag tacccagacg gcacaggagc
     6301 gcacgagcct ggtcgcaggc tacggaagca cgtcgactgc gggctattcc agttccttga
     6361 tcgccggcta tggcagcacg cagacggcag gctacgaaag cacgttgacc gccggttacg
     6421 gcagtacgca aaccgctcag gaaaacagct cgctcaccac aggttacgga agtacttcta
     6481 ctgcgggcta ttccagctcg ctcatcgcgg gttacggcag tacgcaaacg gcaggctacg
     6541 agagcacgtt gaccgccggt tacggtagta cgcaaaccgc gcaggagcgc agtgatctgg
     6601 tgacaggtta tggaagtacc tccaccgccg gctatgcgag ctcgctgatt gcgggttatg
     6661 gcagcacgca gactgcgggt tatgagagca cgttgaccgc cggttacggc agcacgcaaa
     6721 ccgcacagga aaacagctcg ctcaccaccg ggtacggaag tacctccaca gccggctttg
     6781 ccagctcgct gatctccggt tatggcagta cgcagacagc cggctataaa agtaccctca
     6841 cggccggtta cggcagtact cagaccgcag agtatggaag ctcactcact gcgggctacg
     6901 gcagcactgc aacggccggg caggacagtt cattgatagc cggctatggc agctccctga
     6961 ccagcggaat cagaagtttt ctgacggcag gctatggcag tacgctgatc gccggacttc
     7021 gcagcgtttt gatcgccggt tatggcagta gtcttacatc gggcgttcgc agcacgttga
     7081 ctgcgggtta tggcagtaac cagattgcaa gttacggcag ctcgttgatt gcaggccatg
     7141 aaagcattca ggtcgccgga aataaaagca tgctgatcgc cggcaagggc agctcgcaga
     7201 cagcaggttt tcgcagcacg ctgattgccg gtgcgggcag tgtacaactg gcgggtgatc
     7261 gcagccggtt gattgccggt gcagacagta atcagaccgc gggtgaccgc agcaaactgc
     7321 tggccggtaa taacagttat ctgactgccg gcgatagaag caaactgacc ggcgggcatg
     7381 actgcaccct gatggcggga gaccaaagca gattgaccgc tggtaagaac agtgtcttga
     7441 cggcaggcgc tcgtagcaaa ctgattggca gtgaaggctc gacgctctcg gctggagaag
     7501 actccatact tattttcagg ctctgggacg ggaagaggta caggcaactg gtcgccagaa
     7561 cgggtgagaa cggtgttgag gccgacatac cgtattacgt gaacgaagat gacgatattg
     7621 tcgataaacc cgacgaggac gatgactgga tagaggtaaa gtagcccgca attccaggca
     7681 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     7741 ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca
     7801 acggcccgga gggtggcggg caggacgccc gccataaact gccaggaatt gggggcggcc
     7861 ccgtacccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt
     7921 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     7981 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     8041 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     8101 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcat gcataaaaac tgttgtaatt
     8161 cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca
     8221 gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catgggggtg ggcgaagaac
     8281 tccagcatga gatccccgcg ctggaggatc atccagccgg cgtcccggaa aacgattccg
     8341 aagcccaacc tttcatagaa ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc
     8401 gtcgcttggt cggtcatttc gaaccccaga gtcccgctca gaagaactcg tcaagaaggc
     8461 gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg aggaagcggt
     8521 cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct atgtcctgat
     8581 agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg ccattttcca
     8641 ccatgatatt cggcaagcag gcatcgccat gggtcacgac gagatcctcg ccgtcgggca
     8701 tgcgcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca
     8761 gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt
     8821 tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc cgcattgcat
     8881 cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga tcctgccccg
     8941 gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg agcacagctg
     9001 cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtcc tgcagttcat
     9061 tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc
     9121 ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc
     9181 tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg
     9241 atcctcatcc tgtctcttga tcagatcttg atcccctgcg ccatcagatc cttggcggca
     9301 agaaagccat ccagtttact ttgcagggct tcccaacctt accagagggc gccccagctg
     9361 gcaattccgg ttcgcttgct gtccataaaa ccgcccagtc tagctatcgc catgtaagcc
     9421 cactgcaagc tacctgcttt ctctttgcgc ttgcgttttc ccttgtccag atagcccagt
     9481 agctgacatt catcccaggt ggcacttttc ggggaaatgt gcgcgcccgc gttcctgctg
     9541 gcgctgggcc tgtttctggc gctggacttc ccgctgttcc gtcagcagct tttcgcccac
     9601 ggccttgatg atcgcggcgg ccttggcctg catatcccga ttcaacggcc ccagggcgtc
     9661 cagaacgggc ttcaggcgct cccgaaggt
//
