LOCUS       V003931                 9479 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003931
VERSION     V003931
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9479)
  AUTHORS   Wu G, Wang Y, Zhu Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAR-2004) Fish Genetics, Institute of Hydrobiology,
            Chinese Academy of Science, 7# South Road of East Lake, Wuhan, Hubei
            430072, People's Republic of China
REFERENCE   2  (bases 1 to 9479)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 9479)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (09-MAR-2004) Fish Genetics, Institute of Hydrobiology, Chinese
            Academy of Science, 7# South Road of East Lake, Wuhan, Hubei 430072,
            People's Republic of China"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9479
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(419..452)
                     /label="lox71"
                     /note="Left element (LE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      459..2499
                     /label="salmon protamine promoter"
                     /note="salmon protamine promoter"
                     /regulatory_class="promoter"
     regulatory      823..832
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2506..2526
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             2536..5184
                     /note="T7 RNA polymerase from Escherichia phage T7.
                     Accession#: P00573"
                     /label="T7 RNA polymerase"
     polyA_signal    5366..5487
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     enhancer        5597..5900
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5901..6104
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             6212..6928
                     /label="EGFP"
                     /note="enhanced GFP"
     polyA_signal    7054..7175
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     regulatory      7085..7135
                     /label="SV40 polyA"
                     /note="SV40 polyA"
                     /regulatory_class="terminator"
     protein_bind    complement(7188..7221)
                     /label="lox66"
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(7258..7274)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(7282..7298)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7306..7336)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7351..7372)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7660..8248)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8422..9279)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9280..9384)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggta
      421 ccgttcgtat agcatacatt atacgaagtt atggatcatt gctaggagag agtcagagtt
      481 gggcctcttg aacctctgaa ccaggtgtcc ctctggtccc gcccaccagg ctgtgggaac
      541 taacctgtca acattctctg ctgccaatga ctggaacgaa ctgcaaaaat cactgaacct
      601 ggagacccat atctccctca ctagctttaa gcaccagctg tcagagcagc tcacagatca
      661 ctgcacctgt acatagccca catgtaaaca gcccatccaa ctacctcatt cccatactgt
      721 atttatttat cttgctcctt tgcaccccag tatctctact ttcacattca tcttctgcac
      781 atctaccatt ccagtgtcta atttctatat tgtaattact tcgccaccat ggcatattta
      841 ttgccttacc tctcttatct tacctcattt gcacacacta tatagatttt tcttttttct
      901 actgtattat tgactgtatg tttgtttatt ccaggtgtaa ctctgtgttg ttgtatgtgt
      961 cgaactgctg tgctctatct tggccaggtc gcagttgtaa atgagaactt gttctcagct
     1021 tgcctacctg gttaaataaa ataaaaaagt gtcaatcact tgagggttgt catggtatcc
     1081 aggtggagtg ggctcttgaa gatctaccat tttagagagc attctctcta tatgagatca
     1141 aataaaatat gtagaggatg aaatgtttga ctgagtttgg tatttgggaa attaatttta
     1201 tattataccc cattctgata acaatctact gtatagcagc atttgatgag cataatatga
     1261 cttgctttat gcacaacttg tggtgccatt aaatcacaat gcagtttcag tgacatcaca
     1321 acaatctgat tctgagaggc tggttgcagt agttacacag acattctgaa cagtttagct
     1381 gaaagaagct gattcaattg actcctgaga aatcacatga taatagattt aatgagagtg
     1441 cctactcagt agtttagaga ttggttgtaa taaacatatt cagtagtttc agactttcta
     1501 atggatgaca tggctgacat gtcaagggta atagtagagg tcattaataa ttactgcagt
     1561 gggctgaatc agggtcacac ggtgtttcta ggtagtctta aaactacttt gaatcaaaag
     1621 tatacacctc acacacatgg ttatgggcgt gaggtgtata cagaagacac cgtgtaccaa
     1681 gtcagatata gagttgacat gtattacatt ttgagtttgt atcccaatat tacactttta
     1741 tatacatcac agaagactga aatataacaa aaccgtttga catagaaaca ccggattttc
     1801 ggcagctttt aaaaaataat gtgtattaat tatgaaatga tgaatcatat taatgtcatt
     1861 ccacccatga ggctactagg ttatttgact gcaggaaatg gatgattaaa tagactttcc
     1921 ttaaatcctc tgttaaattt tggcataatc aactgaagat gtgttttact gtagtatgat
     1981 agcctatctg tattataata tgctaagcat tctatgctgc agtaggatct cctcttacaa
     2041 cattccaaat caccattaaa tgaagatctg gttgattatt tctaacatgg ttcattgggt
     2101 tggccaaaga aacagattgt tatgggtgta aaatggcaca gtgatgccat ctgttggtaa
     2161 atgttcatta ctgcaactca tgtgttttac cggtgtgctt gagatacccg gatgtattgt
     2221 gatgtactga acaagactgg ttactcgcat caatgcctct ctcgtcattt aacattcaca
     2281 cacagatcac tatttaaaat gacaaaataa aaatatcatt attacatcat cctgccactg
     2341 ctactatgac gtcacataat tcagatgttt tctcaattta aactgtcttt aacacttatt
     2401 gcatcatcat ttatcccata atgacatcac tccagctccc ctccagccct ataaaaggga
     2461 ccaccgcccg tctaaacagt ttatccatca atcacaatgg gatctcctaa gaagaagaga
     2521 aaggtgatcg gatctatgaa cacgattaac atcgctaaga acgacttctc tgacatcgaa
     2581 ctggctgcta tcccgttcaa cactctggct gaccattacg gtgagcgttt agctcgcgaa
     2641 cagttggccc ttgagcatga gtcttacgag atgggtgaag cacgcttccg caagatgttt
     2701 gagcgtcaac ttaaagctgg tgaggttgcg gataacgctg ccgccaagcc tctcatcact
     2761 accctactcc ctaagatgat tgcacgcatc aacgactggt ttgaggaagt gaaagctaag
     2821 cgcggcaagc gcccgacagc cttccagttc ctgcaagaaa tcaagccgga agccgtagcg
     2881 tacatcacca ttaagaccac tctggcttgc ctaaccagtg ctgacaatac aaccgttcag
     2941 gctgtagcaa gcgcaatcgg tcgggccatt gaggacgagg ctcgcttcgg tcgtatccgt
     3001 gaccttgaag ctaagcactt caagaaaaac gttgaggaac aactcaacaa gcgcgtaggg
     3061 cacgtctaca agaaagcatt tatgcaagtt gtcgaggctg acatgctctc taagggtcta
     3121 ctcggtggcg aggcgtggtc ttcgtggcat aaggaagact ctattcatgt aggagtacgc
     3181 tgcatcgaga tgctcattga gtcaaccgga atggttagct tacaccgcca aaatgctggc
     3241 gtagtaggtc aagactctga gactatcgaa ctcgcacctg aatacgctga ggctatcgca
     3301 acccgtgcag gtgcgctggc tggcatctct ccgatgttcc aaccttgcgt agttcctcct
     3361 aagccgtgga ctggcattac tggtggtggc tattgggcta acggtcgtcg tcctctggcg
     3421 ctggtgcgta ctcacagtaa gaaagcactg atgcgctacg aagacgttta catgcctgag
     3481 gtgtacaaag cgattaacat tgcgcaaaac accgcatgga aaatcaacaa gaaagtccta
     3541 gcggtcgcca acgtaatcac caagtggaag cattgtccgg tcgaggacat ccctgcgatt
     3601 gagcgtgaag aactcccgat gaaaccggaa gacatcgaca tgaatcctga ggctctcacc
     3661 gcgtggaaac gtgctgccgc tgctgtgtac cgcaaggaca aggctcgcaa gtctcgccgt
     3721 atcagccttg agttcatgct tgagcaagcc aataagtttg ctaaccataa ggccatctgg
     3781 ttcccttaca acatggactg gcgcggtcgt gtttacgctg tgtcaatgtt caacccgcaa
     3841 ggtaacgata tgaccaaagg actgcttacg ctggcgaaag gtaaaccaat cggtaaggaa
     3901 ggttactact ggctgaaaat ccacggtgca aactgtgcgg gtgtcgataa ggttccgttc
     3961 cctgagcgca tcaagttcat tgaggaaaac cacgagaaca tcatggcttg cgctaagtct
     4021 ccactggaga acacttggtg ggctgagcaa gattctccgt tctgcttcct tgcgttctgc
     4081 tttgagtacg ctggggtaca gcaccacggc ctgagctata actgctccct tccgctggcg
     4141 tttgacgggt cttgctctgg catccagcac ttctccgcga tgctccgaga tgaggtaggt
     4201 ggtcgcgcgg ttaacttgct tcctagtgaa accgttcagg acatctacgg gattgttgct
     4261 aagaaagtca acgagattct acaagcagac gcaatcaatg ggaccgataa cgaagtagtt
     4321 accgtgaccg atgagaacac tggtgaaatc tctgagaaag tcaagctggg cactaaggca
     4381 ctggctggtc aatggctggc ttacggtgtt actcgcagtg tgactaagcg ttcagtcatg
     4441 acgctggctt acgggtccaa agagttcggc ttccgtcaac aagtgctgga agataccatt
     4501 cagccagcta ttgattccgg caagggtctg atgttcactc agccgaatca ggctgctgga
     4561 tacatggcta agctgatttg ggaatctgtg agcgtgacgg tggtagctgc ggttgaagca
     4621 atgaactggc ttaagtctgc tgctaagctg ctggctgctg aggtcaaaga taagaagact
     4681 ggagagattc ttcgcaagcg ttgcgctgtg cattgggtaa ctcctgatgg tttccctgtg
     4741 tggcaggaat acaagaagcc tattcagacg cgcttgaacc tgatgttcct cggtcagttc
     4801 cgcttacagc ctaccattaa caccaacaaa gatagcgaga ttgatgcaca caaacaggag
     4861 tctggtatcg ctcctaactt tgtacacagc caagacggta gccaccttcg taagactgta
     4921 gtgtgggcac acgagaagta cggaatcgaa tcttttgcac tgattcacga ctccttcggt
     4981 accattccgg ctgacgctgc gaacctgttc aaagcagtgc gcgaaactat ggttgacaca
     5041 tatgagtctt gtgatgtact ggctgatttc tacgaccagt tcgctgacca gttgcacgag
     5101 tctcaattgg acaaaatgcc agcacttccg gctaaaggta acttgaacct ccgtgacatc
     5161 ttagagtcgg acttcgcgtt cgcgtaaaga tccactagta acggccgcca gtgtgctgga
     5221 attctgcaga tatccatcac actggcggcc gcgactctag atcataatca gccataccac
     5281 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     5341 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     5401 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     5461 tttgtccaaa ctcatcaatg tatcttaagg cgtagatcct agagggccct ctagatgcat
     5521 gctcgagctc aagctttagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     5581 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     5641 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     5701 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     5761 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     5821 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     5881 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
     5941 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     6001 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
     6061 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
     6121 agatcgggat cagcttgtcg acgtccgcgg agctcctagg cttttgcaaa gatcgatcaa
     6181 gagacaggat gaggatcgtt tcgcaagatc catggtgagc aagggcgagg agctgttcac
     6241 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     6301 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     6361 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
     6421 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     6481 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     6541 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     6601 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     6661 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     6721 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     6781 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     6841 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     6901 cactctcggc atggacgagc tgtacaagta aagcggccgc gactctagat cataatcagc
     6961 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     7021 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     7081 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     7141 agttgtggtt tgtccaaact catcaatgta tcttaaggcg tagatccata acttcgtata
     7201 gcatacatta tacgaacggt agtcgacctg caggcatgca agcttggcgt aatcatggtc
     7261 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     7321 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     7381 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     7441 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     7501 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     7561 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     7621 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     7681 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     7741 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     7801 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     7861 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     7921 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     7981 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     8041 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     8101 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     8161 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     8221 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     8281 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     8341 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     8401 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     8461 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     8521 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     8581 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     8641 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     8701 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     8761 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     8821 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     8881 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     8941 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     9001 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     9061 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     9121 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     9181 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     9241 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     9301 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     9361 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     9421 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtc
//
