LOCUS       40924_35454        9388 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Promoter probe vector pPR9TT, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9388)
  AUTHORS   Santos PM, Blatny JM, Di Bartolo I, Valla S, Zennaro E.
  TITLE     Physiological analysis of the expression of the styrene degradation 
            gene cluster in Pseudomonas fluorescens ST
  JOURNAL   Appl. Environ. Microbiol. 66 (4), 1305-1310 (2000)
  PUBMED    10742204
REFERENCE   2  (bases 1 to 9388)
  AUTHORS   Santos PM, Di Bartolo I, Blatny JM, Zennaro E, Valla S.
  TITLE     New broad-host-range promoter probe vectors based on the plasmid RK2
            replicon
  JOURNAL   FEMS Microbiol. Lett. 195 (1), 91-96 (2001)
  PUBMED    11167001
REFERENCE   3  (bases 1 to 9388)
  AUTHORS   Santos PM, Blatny JM, Di Bartolo I, Valla S, Zennaro E.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-1999) Department of Biology, Third University of 
            Rome, Viale Marconi 446, Rome 00146, Italy
REFERENCE   4  (bases 1 to 9388)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 9388)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2000"; volume: "66"; issue: "4"; pages:
            "1305-1310"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "FEMS 
            Microbiol. Lett."; date: "2001"; volume: "195"; issue: "1"; pages: 
            "91-96"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (16-SEP-1999) Department of Biology, Third University of Rome, Viale
            Marconi 446, Rome 00146, Italy"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9388
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            complement(67..175)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     promoter        352..456
                     /label=AmpR promoter
     CDS             457..1314
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1564..2094
                     /label=oriV
                     /note="incP origin of replication"
     regulatory      2316..2496
                     /label=Pneo
                     /note="Pneo"
                     /regulatory_class="promoter"
     CDS             2486..3631
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     primer_bind     complement(3815..3831)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4327..4983
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     regulatory      5150..5724
                     /label=bidirectional
                     /note="bidirectional"
                     /regulatory_class="terminator"
     primer_bind     5775..5791
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(5813..8857)
                     /label=lacZ
                     /note="beta-galactosidase"
     primer_bind     complement(8897..8913)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(8964..8991)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(9083..9169)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
       61 agcttacggc cagcctcgca gagcaggatt cccgttgagc accgccaggt gcgaataagg
      121 gacagtgaag aaggaacacc cgctcgcggg tgggcctact tcacctatcc tgcccggctg
      181 acgccgttgg atacaccaag gaaagtctac acgaaccctt tggcaaaatc ctgtatatcg
      241 tgcgaaaaag gatggatata ccgaaaaaat cgctataatg accccgaagc agggttatgc
      301 agcggaaaag atccgtcgat cgacccaggt ggcacttttc ggggaaatgt gcgcggaacc
      361 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
      421 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
      481 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
      541 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
      601 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
      661 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
      721 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
      781 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
      841 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
      901 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
      961 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     1021 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     1081 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     1141 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     1201 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     1261 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     1321 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     1381 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     1441 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     1501 tttctgcggg ggatcaggac cgctgccgga gcgcaaccca ctcactacag cagagccatg
     1561 tagggccgcc ggcgttgtgg ataccacgcg gaaaacttgg ccctcactga cagatgaggg
     1621 gcggacgttg acacttgagg ggccgactca cccggcgcgg cgttgacaga tgaggggcag
     1681 gctcgatttc ggccggcgac gtggagctgg ccagcctcgc aaatcggcga aaacgcctga
     1741 ttttacgcga gtttcccaca gatgatgtgg acaagcctgg ggataagtgc cctgcggtat
     1801 tgacacttga ggggcgcgac tactgacaga tgaggggcgc gatccttgac acttgagggg
     1861 cagagtgatg acagatgagg ggcgcaccta ttgacatttg aggggctgtc cacaggcaga
     1921 aaatccagca tttgcaaggg tttccgcccg tttttcggcc accgctaacc tgtcttttaa
     1981 cctgctttta aaccaatatt tataaacctt gtttttaacc agggctgcgc cctggcgcgt
     2041 gaccgcgcac gccgaagggg ggtgcccccc cttctcgaac cctcccggcc cgctaacgcg
     2101 gcaccccatc cccccagggg ctgcgcccct cggccgcgaa cgacctcacc ccaaaaatgg
     2161 cagccacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat gtcagctact
     2221 gggctatctg gacaagggaa aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc
     2281 ttacatggcg atagctagac tgggcggttt tatggacagc aagcgaaccg gaattgccag
     2341 ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt aaactggatg gctttcttgc
     2401 cgccaaggat ctgatggcgc aggggatcaa gatcgacgga tcgatccggg gaattaattc
     2461 cggggcaatc ccgcaaggag ggtgaatgaa tcggacgttt gaccggaagg catacaggca
     2521 agaactgatc gacgcggggt tttccgccga ggatgccgaa accatcgcaa gccgcaccgt
     2581 catgcgtgcg ccccgcgaaa ccttccagtc cgtcggctcg atggtccagc aagctacggc
     2641 caagatcgag cgcgacagcg tgcaactggc tccccctgcc ctgcccgcgc catcggccgc
     2701 cgtggagcgt tcgcgtcgtc tcgaacagga ggcggcaggt ttggcgaagt cgatgaccat
     2761 cgacacgcga ggaactatga cgaccaagaa gcgaaaaacc gccggcgagg acctggcaaa
     2821 acaggtcagc gaggccaagc aggccgcgtt gctgaaacac acgaagcagc agatcaagga
     2881 aatgcagctt tccttgttcg atattgcgcc gtggccggac acgatgcgag cgatgccaaa
     2941 cgacacggcc cgctctgccc tgttcaccac gcgcaacaag aaaatcccgc gcgaggcgct
     3001 gcaaaacaag gtcattttcc acgtcaacaa ggacgtgaag atcacctaca ccggcgtcga
     3061 gctgcgggcc gacgatgacg aactggtgtg gcagcaggtg ttggagtacg cgaagcgcac
     3121 ccctatcggc gagccgatca ccttcacgtt ctacgagctt tgccaggacc tgggctggtc
     3181 gatcaatggc cggtattaca cgaaggccga ggaatgcctg tcgcgcctac aggcgacggc
     3241 gatgggcttc acgtccgacc gcgttgggca cctggaatcg gtgtcgctgc tgcaccgctt
     3301 ccgcgtcctg gaccgtggca agaaaacgtc ccgttgccag gtcctgatcg acgaggaaat
     3361 cgtcgtgctg tttgctggcg accactacac gaaattcata tgggagaagt accgcaagct
     3421 gtcgccgacg gcccgacgga tgttcgacta tttcagctcg caccgggagc cgtacccgct
     3481 caagctggaa accttccgcc tcatgtgcgg atcggattcc acccgcgtga agaagtggcg
     3541 cgagcaggtc ggcgaagcct gcgaagagtt gcgaggcagc ggcctggtgg aacacgcctg
     3601 ggtcaatgat gacctggtgc attgcaaacg ctagggcctt gtggggtcag ttccggctgg
     3661 gggttcagca gccacctgca tcgcaagctg atccgtccaa cgggaatcct gctctgcgag
     3721 gctggccgta agctctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     3781 atcacgaggc cctttcgtct tcaagaatta attcactggc cgtcgtttta caacgtcgtg
     3841 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     3901 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     3961 atggcgaatg gcgccgcgct gatgtccggc ggtgcttttg ccgttacgca ccaccccgtc
     4021 agtagctgaa caggagggac agctgataga aacagaagcc actggcctca aaaacaccat
     4081 catacactaa atcagtaagt tggcagcatc acccgacgca ctttgcgccg aataaatacc
     4141 tgtgacggaa gatcacttcg cagaataaat aaatcctggt gtccctgttg ataccgggaa
     4201 gccctgggcc aacttttggc gaaaatgagg cacgtaagag gttccaactt tcaccataat
     4261 gaaataagat cactaccggg cgtatttttt gagttatcga gattttcagg agctaaggaa
     4321 gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt
     4381 aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag
     4441 ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc
     4501 tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattacgtat ggcaatgaaa
     4561 gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa
     4621 actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac
     4681 atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt
     4741 attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta
     4801 aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg
     4861 caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt ctgtgatggc
     4921 ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg
     4981 gcgtaatttt tttaaggcag ttattggtgc ccttaaacgc ctggttctac gcctgaataa
     5041 gtgataataa gcggatgaat ggcagaaatt cgaaagcaaa ttcgaccccg ctgatcgtgg
     5101 aaacgatagg gaccgacgga tcagcttgct agagggtcag ctttatgctt gtaaaccgtt
     5161 ttgtgaaaaa atttttaaaa taaaaaaggg gacctctagg gtccccaatt aattagtaat
     5221 ataatctatt aaaggtcatt caaaaggtca tccaccggat caattcccct gctcgcgcag
     5281 gctgggtgcc aagctctcgg gtaacatcaa ggcccgatcc ttggtagtcg gcaaataatg
     5341 tctaacaatt cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa gcgttagatg
     5401 cactaagcac ataattgctc acagccaaac tatcaggtca agtctgcttt tattattttt
     5461 aagcgtgcat aataagccct acacaaattg ggagatatat catgaaaggc tggctttttc
     5521 ttgttatcgc aatagttggc gaagtaatcg caacatccgc attaaaatct agcgagggct
     5581 ttactaagct gatccggtgg atgacctttt gaatgacctt taatagatta tattactaat
     5641 taattgggga ccctagaggt cccctttttt attttaaaaa ttttttcaca aaacggttta
     5701 caagcataaa gctgactcta gctagaggat cttcactata gggcgaattg gagctccacc
     5761 gcggtggcgg ccgctctaga actagtggat cccccctgcc cggttattat tatttttgac
     5821 accagaccaa ctggtaatgg tagcgaccgg cgctcagctg gaattccgcc gatactgacg
     5881 ggctccagga gtcgtcgcca ccaatcccca tatggaaacc gtcgatattc agccatgtgc
     5941 cttcttccgc gtgcagcaga tggcgatggc tggtttccat cagttgctgt tgactgtagc
     6001 ggctgatgtt gaactggaag tcgccgcgcc actggtgtgg gccataattc aattcgcgcg
     6061 tcccgcagcg cagaccgttt tcgctcggga agacgtacgg ggtatacatg tctgacaatg
     6121 gcagatccca gcggtcaaaa caggcggcag taaggcggtc gggatagttt tcttgcggcc
     6181 ctaatccgag ccagtttacc cgctctgcta cctgcgccag ctggcagttc aggccaatcc
     6241 gcgccggatg cggtgtatcg ctcgccactt caacatcaac ggtaatcgcc atttgaccac
     6301 taccatcaat ccggtaggtt ttccggctga taaataaggt tttcccctga tgctgccacg
     6361 cgtgagcggt cgtaatcagc accgcatcag caagtgtatc tgccgtgcac tgcaacaacg
     6421 ctgcttcggc ctggtaatgg cccgccgcct tccagcgttc gacccaggcg ttagggtcaa
     6481 tgcgggtcgc ttcacttacg ccaatgtcgt tatccagcgg tgcacgggtg aactgatcgc
     6541 gcagcggcgt cagcagttgt tttttatcgc caatccacat ctgtgaaaga aagcctgact
     6601 ggcggttaaa ttgccaacgc ttattaccca gctcgatgca aaaatccatt tcgctggtgg
     6661 tcagatgcgg gatggcgtgg gacgcggcgg ggagcgtcac actgaggttt tccgccagac
     6721 gccactgctg ccaggcgctg atgtgcccgg cttctgacca tgcggtcgcg ttcggttgca
     6781 ctacgcgtac tgtgagccag agttgcccgg cgctctccgg ctgcggtagt tcaggcagtt
     6841 caatcaactg tttaccttgt ggagcgacat ccagaggcac ttcaccgctt gccagcggct
     6901 taccatccag cgccaccatc cagtgcagga gctcgttatc gctatgacgg aacaggtatt
     6961 cgctggtcac ttcgatggtt tgcccggata aacggaactg gaaaaactgc tgctggtgtt
     7021 ttgcttccgt cagcgctgga tgcggcgtgc ggtcggcaaa gaccagaccg ttcatacaga
     7081 actggcgatc gttcggcgta tcgccaaaat caccgccgta agccgaccac gggttgccgt
     7141 tttcatcata tttaatcagc gactgatcca cccagtccca gacgaagccg ccctgtaaac
     7201 ggggatactg acgaaacgcc tgccagtatt tagcgaaacc gccaagactg ttacccatcg
     7261 cgtgggcgta ttcgcaaagg atcagcgggc gcgtctctcc aggtagcgaa agccattttt
     7321 tgatggacca tttcggcaca gccgggaagg gctggtcttc atccacgcgc gcgtacatcg
     7381 ggcaaataat atcggtggcc gtggtgtcgg ctccgccgcc ttcatactgc accgggcggg
     7441 aaggatcgac agatttgatc cagcgataca gcgcgtcgtg attagcgccg tggcctgatt
     7501 cattccccag cgaccagatg atcacactcg ggtgattacg atcgcgctgc accattcgcg
     7561 ttacgcgttc gctcatcgcc ggtagccagc gcggatcatc ggtcagacga ttcattggca
     7621 ccatgccgtg ggtttcaata ttggcttcat ccaccacata caggccgtag cggtcgcaca
     7681 gcgtgtacca cagcggatgg ttcggataat gcgaacagcg cacggcgtta aagttgttct
     7741 gcttcatcag caggatatcc tgcaccatcg tctgctcatc catgacctga ccatgcagag
     7801 gatgatgctc gtgacggtta acgcctcgaa tcagcaacgg cttgccgttc agcagcagca
     7861 gaccattttc aatccgcacc tcgcggaaac cgacatcgca ggcttctgct tcaatcagcg
     7921 tgccgtcggc ggtgtgcagt tcaaccaccg cacgatagag attcgggatt tcggcgctcc
     7981 acagtttcgg gttttcgacg ttcagacgta gtgtgacgcg atcggcataa ccaccacgct
     8041 catcgataat ttcaccgccg aaaggcgcgg tgccgctggc gacctgcgtt tcaccctgcc
     8101 ataaagaaac tgttacccgt aggtagtcac gcaactcgcc gcacatctga acttcagcct
     8161 ccagtacagc gcggctgaaa tcatcattaa agcgagtggc aacatggaaa tcgctgattt
     8221 gtgtagtcgg tttatgcagc aacgagacgt cacggaaaat gccgctcatc cgccacatat
     8281 cctgatcttc cagataactg ccgtcactcc aacgcagcac catcaccgcg aggcggtttt
     8341 ctccggcgcg taaaaatgcg ctcaggtcaa attcagacgg caaacgactg tcctggccgt
     8401 aaccgaccca gcgcccgttg caccacagat gaaacgccga gttaacgcca tcaaaaataa
     8461 ttcgcgtctg gccttcctgt agccagcttt catcaacatt aaatgtgagc gagtaacaac
     8521 ccgtcggatt ctccgtggga acaaacggcg gattgaccgt aatgggatag gttacgttgg
     8581 tgtagatggg cgcatcgtaa ccgtgcatct gccagtttga ggggacgacg acagtatcgg
     8641 cctcaggaag atcgcactcc agccagcttt ccggcaccgc ttctggtgcc ggaaaccagg
     8701 caaagcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     8761 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
     8821 cgccagggtt ttcccagtca cgacgttgta aaacgacggg atcccccggg ctgcaggaat
     8881 tcgatatcaa gcttatcgat accgtcgacc tcgagggggg gcccggtacc cagcttttgt
     8941 tccctttagt gaagatctac gcaaaaaggc catccgtcag gatggccttc tgcttaattt
     9001 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcgcaa
     9061 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
     9121 agataaaacg aaaggcccag tctttcgact gagcctttcg ttttatttga tgcctggcag
     9181 ttccctactc tcgcatgggg agaccccaca ctaccatcgg cgctacggcg tttcacttct
     9241 gagttcggca tggggtcagg tgggaccacc gcgctactgc cgccaggcaa attctgtttt
     9301 atcagaccgc ttctgcgttc tgatttaatc tgtatcaggc tgaaaatctt ctctcatccg
     9361 ccaggatctg cgaattcctc gaccaatt
//
