LOCUS       62056_18580        6200 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6200
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..45
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             487..513
                     /codon_start=1
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
     primer_bind     complement(611..627)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      630..877
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     promoter        complement(896..914)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(924..940)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1081..1536
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(1637..2308)
                     /codon_start=1
                     /label=TRP1
                     /note="phosphoribosylanthranilate isomerase, required for 
                     tryptophan biosynthesis"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(2309..2589)
                     /label=TRP1 promoter
     rep_origin      2844..4186
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        4213..4317
                     /label=AmpR promoter
     CDS             4318..5175
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5349..5937
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gccgcggatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag
       61 ctctcatttg gcgagcgttg gttggtggat caagcccacg cgtaggcaat cctcgagcag
      121 atccgccagg cgtgtatata tagcgtggat ggccaggcaa ctttagtgct gacacataca
      181 ggcatatata tatgtgtgcg acgacacatg atcatatggc atgcatgtgc tctgtatgta
      241 tataaaactc ttgttttctt cttttctcta aatattcttt ccttatacat taggaccttt
      301 gcagcataaa ttactatact tctatagaca cgcaaacaca aatacacaca ctaatctaga
      361 actagtatgc agattttcgt caagactttg accggtaaaa ccggaacatt ggaagttgaa
      421 tcttccgata ccatcgacaa cgttaagtcg aaaattcaag acaaggaagg aatccctggt
      481 ggtccatacc catacgatgt tccagattac gctggatcca agcagtggta tcaacgcaga
      541 gtggccatta cggcccggga aaaaacatgt cggccgcctc ggcctctcga gaattcgata
      601 tcaagcttat cgataccgtc gacctcgagt catgtaatta gttatgtcac gcttacattc
      661 acgccctccc cccacatccg ctctaaccga aaaggaagga gttagacaac ctgaagtcta
      721 ggtccctatt tattttttta tagttatgtt agtattaaga acgttattta tatttcaaat
      781 ttttcttttt tttctgtaca gacgcgtgta cgcatgtaac attatactga aaaccttgct
      841 tgagaaggtt ttgggacgct cgaaggcttt aatttgcggc cggtacccaa ttcgccctat
      901 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
      961 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     1021 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     1081 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     1141 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     1201 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     1261 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     1321 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     1381 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     1441 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     1501 acgcgaattt taacaaaata ttaacgttta caatttcctg atgcgtattt tctccttacg
     1561 catctgtgcg gtatttcaca ccgcataggc aagtgcacaa acaatactta aataaatact
     1621 actcagtaat aacctatttc ttagcatttt tgacgaaatt tgctattttg ttagagtctt
     1681 ttacaccatt tgtctccaca cctccgctta catcaacacc aataacgcca tttaatctaa
     1741 gcgcatcacc aacattttct ggcgtcagtc caccagctaa cataaaatgt aagctttcgg
     1801 ggctctcttg ccttccaacc cagtcagaaa tcgagttcca atccaaaagt tcacctgtcc
     1861 cacctgcttc tgaatcaaac aagggaataa acgaatgagg tttctgtgaa gctgcactga
     1921 gtagtatgtt gcagtctttt ggaaatacga gtcttttaat aactggcaaa ccgaggaact
     1981 cttggtattc ttgccacgac tcatctccat gcagttggac gatatcaatg ccgtaatcat
     2041 tgaccagagc caaaacatcc tccttaggtt gattacgaaa cacgccaacc aagtatttcg
     2101 gagtgcctga actattttta tatgctttta caagacttga aattttcctt gcaataaccg
     2161 ggtcaattgt tctctttcta ttgggcacac atataatacc cagcaagtca gcatcggaat
     2221 ctagagcaca ttctgcggcc tctgtgctct gcaagccgca aactttcacc aatggaccag
     2281 aactacctgt gaaattaata acagacatac tccaagctgc ctttgtgtgc ttaatcacgt
     2341 atactcacgt gctcaatagt caccaatgcc ctccctcttg gccctctcct tttctttttt
     2401 cgaccgaatt aattcttaat cggcaaaaaa agaaaagctc cggatcaaga ttgtacgtaa
     2461 ggtgacaagc tatttttcaa taaagaatat cttccactac tgccatctgg cgtcataact
     2521 gcaaagtaca catatattac gatgctgtct attaaatgct tcctatatta tatatatagt
     2581 aatgtcgttt atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     2641 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     2701 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     2761 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     2821 tgataataat ggtttcttaa tatgatccaa tatcaaagga aatgatagca ttgaaggatg
     2881 agactaatcc aattgaggag tggcagcata tagaacagct aaagggtagt gctgaaggaa
     2941 gcatacgata ccccgcatgg aatgggataa tatcacagga ggtactagac tacctttcat
     3001 cctacataaa tagacgcata taagtacgca tttaagcata aacacgcact atgccgttct
     3061 tctcatgtat atatatatac aggcaacacg cagatatagg tgcgacgtga acagtgagct
     3121 gtatgtgcgc agctcgcgtt gcattttcgg aagcgctcgt tttcggaaac gctttgaagt
     3181 tcctattccg aagttcctat tctctagaaa gtataggaac ttcagagcgc ttttgaaaac
     3241 caaaagcgct ctgaagacgc actttcaaaa aaccaaaaac gcaccggact gtaacgagct
     3301 actaaaatat tgcgaatacc gcttccacaa acattgctca aaagtatctc tttgctatat
     3361 atctctgtgc tatatcccta tataacctac ccatccacct ttcgctcctt gaacttgcat
     3421 ctaaactcga cctctacatt ttttatgttt atctctagta ttactcttta gacaaaaaaa
     3481 ttgtagtaag aactattcat agagtgaatc gaaaacaata cgaaaatgta aacatttcct
     3541 atacgtagta tatagagaca aaatagaaga aaccgttcat aattttctga ccaatgaaga
     3601 atcatcaacg ctatcacttt ctgttcacaa agtatgcgca atccacatcg gtatagaata
     3661 taatcgggga tgcctttatc ttgaaaaaat gcacccgcag cttcgctagt aatcagtaaa
     3721 cgcgggaagt ggagtcaggc tttttttatg gaagagaaaa tagacaccaa agtagccttc
     3781 ttctaacctt aacggaccta cagtgcaaaa agttatcaag agactgcatt atagagcgca
     3841 caaaggagaa aaaaagtaat ctaagatgct ttgttagaaa aatagcgctc tcgggatgca
     3901 tttttgtaga acaaaaaaga agtatagatt ctttgttggt aaaatagcgc tctcgcgttg
     3961 catttctgtt ctgtaaaaat gcagctcaga ttctttgttt gaaaaattag cgctctcgcg
     4021 ttgcattttt gttttacaaa aatgaagcac agattcttcg ttggtaaaat agcgctttcg
     4081 cgttgcattt ctgttctgta aaaatgcagc tcagattctt tgtttgaaaa attagcgctc
     4141 tcgcgttgca tttttgttct acaaaatgaa gcacagatgc ttcgttcagg tggcactttt
     4201 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     4261 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     4321 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     4381 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     4441 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     4501 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     4561 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     4621 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     4681 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     4741 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     4801 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     4861 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     4921 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     4981 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     5041 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     5101 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     5161 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     5221 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     5281 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     5341 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     5401 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     5461 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     5521 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     5581 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     5641 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     5701 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     5761 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     5821 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     5881 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     5941 gccagcaacg cggcttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     6001 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     6061 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     6121 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     6181 acaggtttcc cgactggaaa
//
