LOCUS       62056_18575        6728 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6728)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        137..155
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        176..880
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             992..1018
                     /codon_start=1
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
     terminator      1158..1405
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     promoter        complement(1424..1442)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1452..1468)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1609..2064
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(2165..2836)
                     /codon_start=1
                     /label=TRP1
                     /note="phosphoribosylanthranilate isomerase, required for 
                     tryptophan biosynthesis"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(2837..3117)
                     /label=TRP1 promoter
     rep_origin      3372..4714
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        4741..4845
                     /label=AmpR promoter
     CDS             4846..5703
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5877..6465
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gccgcggatg attacgccaa gcgcgcaatt aaccaatacg caaaccgcct ctccccgcgc
       61 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gccgcggatg
      121 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctcatatcct
      181 tttgttgttt ccgggtgtac aatatggact tcctcttttc tggcaaccaa acccatacat
      241 cgggattcct ataatacctt cgttggtctc cctaacatgt aggtggcgga ggggagatat
      301 acaatagaac agataccaga caagacataa tgggctaaac aagactacac caattacact
      361 gcctcattga tggtggtaca taacgaacta atactgtagc cctagacttg atagccatca
      421 tcatatcgaa gtttcactac cctttttcca tttgccatct attgaagtaa taataggcgc
      481 atgcaacttc ttttcttttt ttttcttttc tctctccccc gttgttgtct caccatatcc
      541 gcaatgacaa aaaaatgatg gaagacacta aaggaaaaaa ttaacgacaa agacagcacc
      601 aacagatgtc gttgttccag agctgatgag gggtatctcg aagcacacga aactttttcc
      661 ttccttcatt cacgcacact actctctaat gagcaacggt atacggcctt ccttccagtt
      721 acttgaattt gaaataaaaa aaagtttgct gtcttgctat caagtataaa tagacctgca
      781 attattaatc ttttgtttcc tcgtcattgt tctcgttccc tttcttcctt gtttcttttt
      841 ctgcacaata tttcaagcta taccaagcat acaatcaact cactagtgga tccaagcagt
      901 ggtatcaacg cagagtggcc attacggccc gggaaaaaac atgtcggccg cctcggcctc
      961 tcgagaattc gatatcaagc ttatcgatat gtacccatac gatgttccag attacgctcc
     1021 tgcagccatg cagattttcg tcaagacttt gaccggtaaa accggaacat tggaagttga
     1081 atcttccgat accatcgaca acgttaagtc gaaaattcaa gacaaggaag gaatccctgg
     1141 tggatgtcga cctcgagtca tgtaattagt tatgtcacgc ttacattcac gccctccccc
     1201 cacatccgct ctaaccgaaa aggaaggagt tagacaacct gaagtctagg tccctattta
     1261 tttttttata gttatgttag tattaagaac gttatttata tttcaaattt ttcttttttt
     1321 tctgtacaga cgcgtgtacg catgtaacat tatactgaaa accttgcttg agaaggtttt
     1381 gggacgctcg aaggctttaa tttgcggccg gtacccaatt cgccctatag tgagtcgtat
     1441 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     1501 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     1561 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggac gcgccctgta
     1621 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     1681 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     1741 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     1801 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     1861 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     1921 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     1981 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     2041 acaaaatatt aacgtttaca atttcctgat gcgtattttc tccttacgca tctgtgcggt
     2101 atttcacacc gcataggcaa gtgcacaaac aatacttaaa taaatactac tcagtaataa
     2161 cctatttctt agcatttttg acgaaatttg ctattttgtt agagtctttt acaccatttg
     2221 tctccacacc tccgcttaca tcaacaccaa taacgccatt taatctaagc gcatcaccaa
     2281 cattttctgg cgtcagtcca ccagctaaca taaaatgtaa gctttcgggg ctctcttgcc
     2341 ttccaaccca gtcagaaatc gagttccaat ccaaaagttc acctgtccca cctgcttctg
     2401 aatcaaacaa gggaataaac gaatgaggtt tctgtgaagc tgcactgagt agtatgttgc
     2461 agtcttttgg aaatacgagt cttttaataa ctggcaaacc gaggaactct tggtattctt
     2521 gccacgactc atctccatgc agttggacga tatcaatgcc gtaatcattg accagagcca
     2581 aaacatcctc cttaggttga ttacgaaaca cgccaaccaa gtatttcgga gtgcctgaac
     2641 tatttttata tgcttttaca agacttgaaa ttttccttgc aataaccggg tcaattgttc
     2701 tctttctatt gggcacacat ataataccca gcaagtcagc atcggaatct agagcacatt
     2761 ctgcggcctc tgtgctctgc aagccgcaaa ctttcaccaa tggaccagaa ctacctgtga
     2821 aattaataac agacatactc caagctgcct ttgtgtgctt aatcacgtat actcacgtgc
     2881 tcaatagtca ccaatgccct ccctcttggc cctctccttt tcttttttcg accgaattaa
     2941 ttcttaatcg gcaaaaaaag aaaagctccg gatcaagatt gtacgtaagg tgacaagcta
     3001 tttttcaata aagaatatct tccactactg ccatctggcg tcataactgc aaagtacaca
     3061 tatattacga tgctgtctat taaatgcttc ctatattata tatatagtaa tgtcgtttat
     3121 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
     3181 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     3241 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     3301 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     3361 tttcttaata tgatccaata tcaaaggaaa tgatagcatt gaaggatgag actaatccaa
     3421 ttgaggagtg gcagcatata gaacagctaa agggtagtgc tgaaggaagc atacgatacc
     3481 ccgcatggaa tgggataata tcacaggagg tactagacta cctttcatcc tacataaata
     3541 gacgcatata agtacgcatt taagcataaa cacgcactat gccgttcttc tcatgtatat
     3601 atatatacag gcaacacgca gatataggtg cgacgtgaac agtgagctgt atgtgcgcag
     3661 ctcgcgttgc attttcggaa gcgctcgttt tcggaaacgc tttgaagttc ctattccgaa
     3721 gttcctattc tctagaaagt ataggaactt cagagcgctt ttgaaaacca aaagcgctct
     3781 gaagacgcac tttcaaaaaa ccaaaaacgc accggactgt aacgagctac taaaatattg
     3841 cgaataccgc ttccacaaac attgctcaaa agtatctctt tgctatatat ctctgtgcta
     3901 tatccctata taacctaccc atccaccttt cgctccttga acttgcatct aaactcgacc
     3961 tctacatttt ttatgtttat ctctagtatt actctttaga caaaaaaatt gtagtaagaa
     4021 ctattcatag agtgaatcga aaacaatacg aaaatgtaaa catttcctat acgtagtata
     4081 tagagacaaa atagaagaaa ccgttcataa ttttctgacc aatgaagaat catcaacgct
     4141 atcactttct gttcacaaag tatgcgcaat ccacatcggt atagaatata atcggggatg
     4201 cctttatctt gaaaaaatgc acccgcagct tcgctagtaa tcagtaaacg cgggaagtgg
     4261 agtcaggctt tttttatgga agagaaaata gacaccaaag tagccttctt ctaaccttaa
     4321 cggacctaca gtgcaaaaag ttatcaagag actgcattat agagcgcaca aaggagaaaa
     4381 aaagtaatct aagatgcttt gttagaaaaa tagcgctctc gggatgcatt tttgtagaac
     4441 aaaaaagaag tatagattct ttgttggtaa aatagcgctc tcgcgttgca tttctgttct
     4501 gtaaaaatgc agctcagatt ctttgtttga aaaattagcg ctctcgcgtt gcatttttgt
     4561 tttacaaaaa tgaagcacag attcttcgtt ggtaaaatag cgctttcgcg ttgcatttct
     4621 gttctgtaaa aatgcagctc agattctttg tttgaaaaat tagcgctctc gcgttgcatt
     4681 tttgttctac aaaatgaagc acagatgctt cgttcaggtg gcacttttcg gggaaatgtg
     4741 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     4801 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     4861 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     4921 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     4981 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     5041 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     5101 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     5161 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     5221 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     5281 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     5341 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     5401 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     5461 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     5521 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     5581 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     5641 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     5701 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     5761 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     5821 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     5881 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     5941 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     6001 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     6061 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     6121 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     6181 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     6241 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     6301 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     6361 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     6421 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     6481 gctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     6541 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     6601 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     6661 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     6721 actggaaa
//
